Electrochemical pesticide sensitivity test using acetylcholinesterase biosensor based on colloidal gold nanoparticle modified sol-gel interface.

Based on the change in electrochemical behavior of enzymatic activity induced by pesticide, a novel electrochemical method for investigation of pesticide sensitivity using acetylcholinesterase (AChE) biosensor was developed. The sol-gel-derived silicate network assembling gold nanoparticles (AuNPs-SiSG) provided a biocompatible microenvironment around the enzyme molecule to stabilize its biological activity and prevented them from leaking out of the interface. The composite was characterized using atomic force microscopy and proved to be chemically clean, porous and homogeneous. AuNPs promoted a conductive pathway for electron transfer and improved electrochemical reactions at a lower potential. Typical pesticides such as monocrotophos, methyl parathion and carbaryl were selected for pesticide sensitivity tests. Due to the inhibitions of pesticides, the electrochemical responses of substrate on AChE-sensors decreased greatly. The inhibition curves showed good correspondence with the results by UV spectrophotometry assay. The proposed electrochemical pesticide sensitivity test exhibited high sensitivity, desirable accuracy, low cost and simplified procedures. This method could be developed as a conventional method to select efficient enzyme inhibitors and investigate toxic compounds against to enzyme.

[1]  Jean-Louis Marty,et al.  Screen-printed electrode based on AChE for the detection of pesticides in presence of organic solvents. , 2002, Talanta.

[2]  C. Choi,et al.  Glucose sensor using a microfabricated electrode and electropolymerized bilayer films. , 2002, Biosensors & bioelectronics.

[3]  I. Willner,et al.  An Os(II)--bisbipyridine--4-picolinic acid complex mediates the biocatalytic growth of au nanoparticles: optical detection of glucose and acetylcholine esterase inhibition. , 2005, Chemistry.

[4]  I. Willner,et al.  Growing Metal Nanoparticles by Enzymes , 2006 .

[5]  D. Astruc,et al.  Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. , 2004, Chemical reviews.

[6]  H. Ju,et al.  Reagentless amperometric immunosensors based on direct electrochemistry of horseradish peroxidase for determination of carcinoma antigen-125. , 2003, Analytical chemistry.

[7]  Jonathan M. Cooper,et al.  Biosensors : a practical approach , 2004 .

[8]  Dan Du,et al.  An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube–cross-linked chitosan composite , 2007, Analytical and bioanalytical chemistry.

[9]  J Emnéus,et al.  Multienzyme electrochemical array sensor for determination of phenols and pesticides. , 2005, Talanta.

[10]  Guan-Hai Wang,et al.  Using novel polysaccharide-silica hybrid material to construct an amperometric biosensor for hydrogen peroxide. , 2006, The journal of physical chemistry. B.

[11]  S. S. Soares,et al.  Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration. , 2007, Journal of inorganic biochemistry.

[12]  L. Blum,et al.  Protein microarrays enhanced performance using nanobeads arraying and polymer coating. , 2007, Talanta.

[13]  Baoxin Li,et al.  Simultaneous determination of three organophosphorus pesticides residues in vegetables using continuous-flow chemiluminescence with artificial neural network calibration. , 2007, Talanta.

[14]  B. Jena,et al.  Electrochemical biosensor based on integrated assembly of dehydrogenase enzymes and gold nanoparticles. , 2006, Analytical chemistry.

[15]  Dan Du,et al.  Electrochemical immunoassay of membrane P-glycoprotein by immobilization of cells on gold nanoparticles modified on a methoxysilyl-terminated butyrylchitosan matrix. , 2005, Biochemistry.

[16]  A. Bard,et al.  Ellipsometric, electrochemical, and elemental characterization of the surface phase produced on glassy carbon electrodes by electrochemical activation , 1988 .

[17]  G. Theodoridis,et al.  Solid phase microextraction applied to the analysis of organophosphorus insecticides in fruits. , 2006, Chemosphere.

[18]  Dan Du,et al.  Immunological assay for carbohydrate antigen 19-9 using an electrochemical immunosensor and antigen immobilization in titania sol-gel matrix. , 2003, Journal of immunological methods.

[19]  Huangxian Ju,et al.  Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer , 1999 .

[20]  A. B. Garmarudi,et al.  Quantitative determination of Malathion in pesticide by modified attenuated total reflectance-Fourier transform infrared spectrometry applying genetic algorithm wavelength selection method. , 2007, Talanta.

[21]  Danila Moscone,et al.  Detection of carbamic and organophosphorous pesticides in water samples using a cholinesterase biosensor based on Prussian Blue-modified screen-printed electrode. , 2006, Analytica chimica acta.

[22]  Song Zhang,et al.  A mediator-free screen-printed amperometric biosensor for screening of organophosphorus pesticides with flow-injection analysis (FIA) system. , 2006, Talanta.

[23]  A. Scozzafava,et al.  Perspectives in bioremediation: technologies for environmental improvement. , 1997 .

[24]  Zhiyong Tang,et al.  Multicolor luminescence patterning by photoactivation of semiconductor nanoparticle films. , 2003, Journal of the American Chemical Society.

[25]  D. Milori,et al.  Influence of humic substances on the photolysis of aqueous pesticide residues. , 2007, Chemosphere.