In vivo andin vitro HeNe laser effects on phagocyte functions

The goal of this work was to evaluate the effect of helium-neon (HeNe) laser irradiation on immunocompetent cells. We used the in vivo skin window method and in vitro granulocyte function tests. The study of cellular migration showed a marked decrease in vitro and in vivo in a dose-independent manner. Superoxide release was not modified by laser irradiation. The granulocyte's aggregation, when using PHA and PMA, presented a reduction that was statistically very significant, not as a subordinate dose. An increase of the release of ATP was demonstrated only at 4 joules and precedes granulocyte aggregation. When using Ca2+ ionophore A23187 as stimulus, laser irradiation at 1, 2 or 4J did not show any modification of granulocyte aggregation. The monoclonal antibody 60.1, which identifies a membrane antigen fundamental for aggregation and chemotaxis, is expressed in normal amounts on granulocyte membranes both before and after irradiation with a HeNe laser. In fact, laser irradiation preferentially attacks the area of the cellular centrosome that determines a modification of cellular morphology. The electron microscope and immunofluorescence study with a monoclonal antibody have pointed out a disorganization of the microtubles. The alteration of some of the granulocyte functions is correlated to the damage in the centrioles. The granulocyte mitocondrial system and surface membrane remain intact, and this explains the normal production and release of free radicals. Further experiments are necessary to evaluate the clinical application of lasers in various diseases with immunophagocytic pathogenesis.

[1]  K. Hellman,et al.  ULTRAVIOLET RADIATION INDUCED CHANGES IN MACROPHAGE ACTIVITY , 1981, Photochemistry and photobiology.

[2]  C. Haun,et al.  Laser therapy of rheumatoid arthritis , 1980, Lasers in surgery and medicine.

[3]  E. Mester,et al.  Stimulation of wound healing by means of laser rays. Part III--Investigation of the effect on immune competent cells. , 1978, Acta chirurgica Academiae Scientiarum Hungaricae.

[4]  G. Buffone,et al.  Use of a laser-equipped centrifugal analyzer for kinetic measurement of serum IgG. , 1974, Clinical chemistry.

[5]  G. Ricevuti,et al.  Clinical aspects of neutrophil locomotion disorders. , 1987, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[6]  G. Ricevuti,et al.  Prognostic significant of functional defects of granulocytes in myeloproliferative disease. , 1986, Oncology.

[7]  Mann Dp.  BASIC ASPECTS OF LASER OPERATION. , 1965 .

[8]  G. Ricevuti,et al.  The Biology of phagocytes in health and disease , 1987 .

[9]  S. Sacchi,et al.  In vitro fibroblast and dermis fibroblast activation by laser irradiation at low energy. An electron microscopic study. , 1984, Dermatologica.

[10]  M. Koonce,et al.  Laser irradiation of centrosomes in newt eosinophils: evidence of centriole role in motility , 1984, The Journal of cell biology.

[11]  J. Schaller,et al.  Job's Syndrome. Recurrent, "cold", staphylococcal abscesses. , 1966 .

[12]  A. Böyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[13]  C. McCall,et al.  Neutrophil Aggregation: Evidence for a Different Mechanism of Action by Phorbol Myristate Acetate , 1980, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[14]  B. Babior,et al.  Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. , 1973, The Journal of clinical investigation.

[15]  P. Quie.  Infections in patients with abnormal granulocyte chemotaxis. , 1981 .

[16]  J. Lubowsky,et al.  The lumi-aggregometer: a new instrument for simultaneous measurement of secretion and aggregation by platelets. , 1977, The Journal of laboratory and clinical medicine.

[17]  M. Mardiney,et al.  Quantitative and qualitative analysis of human lymphocyte proliferation to specific antigen in vitro by use of the helium neon laser. , 1977, Journal of immunological methods.

[18]  N. Trapeznikov,et al.  [Action of helium-neon lasers on human lymphocytes in vitro]. , 1984, Vestnik Akademii meditsinskikh nauk SSSR.

[19]  A. Bøyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. , 1968 .

[20]  G. Weissmann,et al.  Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis. , 1975, The Journal of clinical investigation.

[21]  Williams Ea.  Letter: Caesarean section. , 1976 .

[22]  H. Hill,et al.  Raised serum-IgE levels and defective neutrophil chemotaxis in three children with eczema and recurrent bacterial infections. , 1974, Lancet.

[23]  D. Apfelberg,et al.  Extended clinical use of the argon laser for cutaneous lesions , 1980, Archives of dermatology.

[24]  S. Solomon,et al.  Activation of cyclic AMP phosphodiesterase by phorbol and protein kinase C pathway. , 1986, The American journal of the medical sciences.

[25]  M. Roch-Arveiller,et al.  Modification of neutrophil chemotactic responsiveness during various inflammatory reactions. , 1985, Pathology, research and practice.

[26]  A. Korenyi-both,et al.  Stimulation of wound healing by means of laser rays. (Clinical and electron microscopical study). , 1973, Acta chirurgica Academiae Scientiarum Hungaricae.

[27]  J. Gallin,et al.  Structural analysis of human neutrophil migration: Centriole, microtubule, and microfilament orientation and function during chemotaxis , 1977, The Journal of cell biology.

[28]  G. Ricevuti,et al.  Membrane glycoproteins of neutrophils and related diseases. , 1988, Haematologica.

[29]  J. White,et al.  Complement (C5-a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia. , 1977, The Journal of clinical investigation.

[30]  D. Apfelberg,et al.  Treatment of nevi aranei by means of an argon laser. , 1978, The Journal of dermatologic surgery and oncology.

[31]  J. P. Hulsbergen Henning,et al.  A model approach to laser coagulation of dermal vascular lesions , 2004, Archives of Dermatological Research.

[32]  P. Ward.  Leukotaxis and leukotactic disorders. A review. , 1974, The American journal of pathology.

[33]  G. Geller,et al.  Recurrent severe staphylococcal infections, eczematoid rash, extreme elevations of IgE, eosinophilia, and divergent chemotactic responses in two generations. , 1977, The Journal of pediatrics.

[34]  S. Abramson,et al.  Inhibition of neutrophil activation by nonsteroidal anti-inflammatory drugs. , 1984, The American journal of medicine.

[35]  G. Fischer,et al.  Decreased Phytohemagglutinin-Induced Aggregation and C5a-Induced Chemotaxis of Human Newborn Neutrophils , 1980, Pediatric Research.

[36]  P. Quie,et al.  Neutrophil chemotaxis in patients with atopic dermatitis without infection. , 1978, Archives of dermatology.

[37]  L. Goldman,et al.  Laser Treatment of Tattoos: A Preliminary Survey of Three Year's Clinical Experience , 1967 .

[38]  G. Ricevuti,et al.  Definition of CD 11a, b, c, and CD 18 glycoproteins on chemotactically deficient granulocyte membranes in patients affected by myeloid disorders. , 1989, Acta haematologica.

[39]  P. Fratino,et al.  The Free Radicals by Granulocytes in Non Infectious Diseases , 1988 .

[40]  E. Mester,et al.  THE EFFECT OF LASER RADIATION ON WOUND HEALING AND COLLAGEN SYNTHESIS , 1971 .

[41]  B. Samuelsson.  Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation. , 1983, Science.

[42]  D. Evans,et al.  NEW PATHOLOGICAL INSTITUTE AT THE LONDON. , 1965, Lancet.

[43]  A. Bird,et al.  Photocoagulation of disciform macular lesions with krypton laser. , 1979, The British journal of ophthalmology.

[44]  Sally H. Zigmond,et al.  Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. , 1973 .

[45]  B. Samuelsson.  LEUKOTRIENES: MEDIATORS OF IMMEDIATE HYPERSENSITIVITY REACTIONS AND INFLAMMATION , 1983 .

[46]  D. Apfelberg,et al.  Extended clinical use of the argon laser for cutaneous lesions. , 1979, Archives of dermatology.

[47]  H. Perez,et al.  An immunoglobulin (IgG) inhibitor of polymorphonuclear leukocyte motility in a patient with recurrent infection. , 1980, The New England journal of medicine.

[48]  J. Kosek,et al.  Histology of port wine stains following argon laser treatment. , 1979, British journal of plastic surgery.

[49]  S. Klebanoff.  Oxygen metabolism and the toxic properties of phagocytes. , 1980, Annals of internal medicine.