Effect of various compounds on the Ehrlich ascites carcinoma.

1. The effects of nitrogen mustards, phosphor-amides, purines, pyrimidines, triazines, folic acid analogs, pteridines, steroids, protein hormones, antivitamins, antibiotics, and miscellaneous compounds were studied on the growth of Ehrlich ascites tumor. 2. Repeated subcutaneous or intraperitoneal injections of HN2 or HN2 oxide had a destructive effect on the tumor ascites but none, or nearly none, on the solid form. 3. Triethylene melamine, triethylene phosphoramide, diethylene phosphoramide, and a methoxy pyridoxine mustard had a marked inhibitory effect on the development of tumor ascites when injected intraperitoneally. 4. Subcutaneous injections of cortisone had a destructive effect on tumor ascites, while intraperitoneal injections had but slight effect. Compound F appears to be nearly as active as cortisone, whereas other steroids tested have shown no activity on Ehrlich ascites tumor. 5. Intraperitoneal injections of 6-mercaptopurine had a marked inhibitory effect on Ehrlich mouse carcinoma, both in the solid and ascitic forms. 6. Repeated intraperitoneal injections of Shear's bacterial polysaccharide had a marked inhibitory effect on the development of tumor ascites, but had no effect if given subcutaneously. 7. Colchicine had a slight inhibitory effect on Ehrlich ascites tumor, but urethan and podophyl-lotoxin had no inhibitory effect. 8. 2,6-Diaminopurine, 8-azaguanine, and five pyrimidines tested had no inhibitory effect on Ehrlich mouse carcinoma, whether in solid or ascitic form. 9. Daily subcutaneous or intraperitoneal injections of folic acid, aminopterin, and A-methopterin had no inhibitory effects either on the solid or on the ascitic form of Ehrlich mouse carcinoma. 10. Repeated intraperitoneal injections of pure antibiotics, penicillin, streptomycin, aureomycin, chloromycetin, and terramycin had neither inhibiting nor stimulating effects upon the development of Ehrlich ascites tumor in mice. 11. The Ehrlich ascites tumor is a useful tool for testing the activity of chemicals.