Conservation of a hexapeptide sequence in the anchor region of surface proteins from Gram‐positive cocci

A highly conserved hexapeptide sequence (both at the protein‐and DNA level) has been identified within the C‐terminal end of all 11 known surface proteins from Gram‐positive cocci. The hexapeptide, with the consensus sequence LPXTGE, is located about 9 amino acids N‐terminal from the C‐terminal hydrophobic domain which is found in all these surface molecules. The conservation of the hexapeptide, despite sequence variation within the regions flanking it, suggests that it is important for the attachment of these proteins within the cell.

[1]  M. Uhlén,et al.  Region X, the cell-wall-attachment part of staphylococcal protein A. , 1984, European journal of biochemistry.

[2]  B Guss,et al.  Structure and evolution of the repetitive gene encoding streptococcal protein G. , 1987, European journal of biochemistry.

[3]  P. Cleary,et al.  Fc-receptor and M-protein genes of group A streptococci are products of gene duplication. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[4]  V. Fischetti,et al.  Sequence and structural characteristics of the trypsin-resistant T6 surface protein of group A streptococci , 1990, Journal of bacteriology.

[5]  G. Blobel,et al.  Intracellular protein topogenesis. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[6]  G. Lindahl,et al.  Extensive sequence homology between IgA receptor and M proteins in Streptococcus pyogenes , 1989, Molecular microbiology.

[7]  M. Lindberg,et al.  Nucleotide sequence of the gene for a fibronectin-binding protein from Staphylococcus aureus: use of this peptide sequence in the synthesis of biologically active peptides. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[8]  G. Venema,et al.  Nucleotide sequence of the cell wall proteinase gene of Streptococcus cremoris Wg2 , 1988, Applied and environmental microbiology.

[9]  V. Fischetti,et al.  Isolation and characterization of the cell-associated region of group A streptococcal M6 protein , 1988, Journal of bacteriology.

[10]  J. Ferretti,et al.  Sequence analysis of the wall‐associated protein precursor of Streptococcus mutans antigen A , 1989, Molecular microbiology.

[11]  C. Sasakawa,et al.  Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental caries , 1989, Molecular microbiology.

[12]  V. Fischetti,et al.  Complete nucleotide sequence of type 6 M protein of the group A Streptococcus. Repetitive structure and membrane anchor. , 1986, The Journal of biological chemistry.

[13]  M. Ferguson,et al.  Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures. , 1988, Annual review of biochemistry.