The effects of atmospheric pressure cold plasma treatment on microbiological, physical-chemical and sensory characteristics of vacuum packaged beef loin.

Effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (ACP) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. Exposure of non-covered beef samples under high power ACP conditions resulted in increased a*, b*, Chroma and Hue values, but ACP treatment of packaged loins did not impact colour (L*, a*, b*, Chroma, Hue), lipid peroxidation, sarcoplasmic protein denaturation, nitrate/nitrite uptake, or myoglobin isoform distribution. Colour values measured after 3days of aerobic storage following unpackaging (i.e. 20days post-mortem) were similar and all compliant with consumer acceptability standards. Exposure to ACP of the polyamide-polyethylene packaging film inoculated with Staphylococcus aureus, Listeria monocytogenes and two Escherichia coli strains resulted in >2 log reduction without affecting the integrity of the packaging matrix. Results indicate that ACP can reduce microbial numbers on surfaces of beef packages without affecting characteristics of the packaged beef.

[1]  M. Rong,et al.  Aqueous reactive species induced by a surface air discharge: Heterogeneous mass transfer and liquid chemistry pathways , 2016, Scientific Reports.

[2]  G. F. Krause,et al.  A NEW EXTRACTION METHOD FOR DETERMINING 2‐THIOBARBITURIC ACID VALUES OF PORK AND BEEF DURING STORAGE , 1970 .

[3]  J. Walsh,et al.  A solar powered handheld plasma source for microbial decontamination applications , 2016 .

[4]  L. Arroja,et al.  Biosorption of Milk Substrates onto Anaerobic Flocculent and Granular Sludge , 2003, Biotechnology progress.

[5]  M. Meyyappan,et al.  Impact of Low‐Temperature Plasmas on Deinococcusradiodurans and Biomolecules , 2003, Biotechnology progress.

[6]  P. Chu,et al.  Effects and Mechanism of Atmospheric-Pressure Dielectric Barrier Discharge Cold Plasma on Lactate Dehydrogenase (LDH) Enzyme , 2015, Scientific Reports.

[7]  P. Puligundla,et al.  Corona discharge plasma jet for inactivation of Escherichia coli O157:H7 and Listeria monocytogenes on inoculated pork and its impact on meat quality attributes , 2015, Annals of Microbiology.

[8]  S. McMahon,et al.  Cold atmospheric pressure plasma jet interactions with plasmid DNA , 2011 .

[9]  R. Poonkhum,et al.  Effects of ozone treatment on cell growth and ultrastructural changes in bacteria. , 2002, The Journal of general and applied microbiology.

[10]  W. Stickle,et al.  Handbook of X-Ray Photoelectron Spectroscopy , 1992 .

[11]  W. Choe,et al.  Flexible thin-layer dielectric barrier discharge plasma treatment of pork butt and beef loin: effects on pathogen inactivation and meat-quality attributes. , 2015, Food microbiology.

[12]  W. Choe,et al.  Evaluation of pathogen inactivation on sliced cheese induced by encapsulated atmospheric pressure dielectric barrier discharge plasma. , 2015, Food microbiology.

[13]  H. J. Swatland,et al.  On-line evaluation of meat , 1995 .

[14]  David B. Graves,et al.  Long-term antibacterial efficacy of air plasma-activated water , 2011 .

[15]  D. J. Buckley,et al.  Oxidative quality and shelf life of meats. , 1996, Meat science.

[16]  Zhenxiang Chen,et al.  Sterilization and plasma processing of room temperature surfaces with a one atmosphere uniform glow discharge plasma (OAUGDP) , 2000 .

[17]  K. Marycz,et al.  Antimicrobial activity of low-pressure plasma treatment against selected foodborne bacteria and meat microbiota , 2014, Annals of Microbiology.

[18]  J. Walsh,et al.  Optimizing the electrical excitation of an atmospheric pressure plasma advanced oxidation process. , 2014, Journal of hazardous materials.

[19]  Christophe Leys,et al.  Application of atmospheric pressure dielectric barrier discharges in deposition, cleaning and activation. , 2001 .

[20]  A. Peters,et al.  Incidence of Campylobacter and Salmonella isolated from retail chicken and associated packaging in South Wales , 2001, Letters in applied microbiology.

[21]  K. Lundström,et al.  Variation in light scattering and water-holding capacity along the porcine Longissimus dorsi muscle. , 1985, Meat science.

[22]  Thomas von Woedtke,et al.  Analysis of antibacterial efficacy of plasma-treated sodium chloride solutions , 2015 .

[23]  H. Crémer,et al.  Fleisch und fleischwaren , 1944 .

[24]  A. A. Bekhit,et al.  Oxidative Processes in Muscle Systems and Fresh Meat: Sources, Markers, and Remedies. , 2013, Comprehensive reviews in food science and food safety.

[25]  R. Klont,et al.  Muscle fibre type and meat quality. , 1998, Meat science.

[26]  A. Bekhit,et al.  Metmyoglobin reducing activity. , 2005, Meat science.

[27]  D. Ahn,et al.  Mechanism of Lipid Peroxidation in Meat and Meat Products -A Review , 2005 .

[28]  F. Smulders,et al.  The Conversion of Muscle to Meat , 2014 .

[29]  E. Huff-Lonergan,et al.  Oxidative environments decrease tenderization of beef steaks through inactivation of mu-calpain. , 2004, Journal of animal science.

[30]  K. Ishikawa,et al.  Cold plasma interactions with enzymes in foods and model systems , 2016 .

[31]  F. Smulders,et al.  Variation in post-mortem rate of glycolyis does not necessarily affect drip loss of non-stimulated veal. , 1997, Meat science.

[32]  T. Hindré,et al.  Shiga Toxin Produced by Enterohemorrhagic Escherichia coli Inhibits PI3K/NF-κB Signaling Pathway in Globotriaosylceramide-3-Negative Human Intestinal Epithelial Cells1 , 2007, The Journal of Immunology.

[33]  S. W. Souci,et al.  Food composition and nutrition tables. , 2000 .

[34]  James L. Walsh,et al.  Numerical investigation of the spatiotemporal distribution of chemical species in an atmospheric surface barrier-discharge , 2016 .

[35]  D. Knorr,et al.  Cold plasma effects on enzyme activity in a model food system , 2013 .

[36]  W. Choe,et al.  Inactivation of Listeria monocytogenes inoculated on disposable plastic tray, aluminum foil, and paper cup by atmospheric pressure plasma. , 2010 .

[37]  Wonho Choe,et al.  The use of atmospheric pressure plasma-treated water as a source of nitrite for emulsion-type sausage. , 2015, Meat science.

[38]  Qun Sun,et al.  Myoglobin and lipid oxidation interactions: mechanistic bases and control. , 2010, Meat science.

[39]  R. G. Kauffman,et al.  The relationship of sarcoplasmic and myofibrillar protein solubility to colour and water-holding capacity in porcine longissimus muscle. , 1999, Meat science.

[40]  R. Cassens,et al.  THE BIOCHEMICAL BASIS FOR DISCOLORATION IN FRESH MEAT: A REVIEW , 1990 .

[41]  James L. Walsh,et al.  10 ns pulsed atmospheric air plasma for uniform treatment of polymeric surfaces , 2007 .

[42]  P. Murányi,et al.  Sterilization efficiency of a cascaded dielectric barrier discharge , 2007, Journal of applied microbiology.

[43]  D. Liebler,et al.  Induction of redox instability of bovine myoglobin by adduction with 4-hydroxy-2-nonenal. , 2003, Biochemistry.

[44]  F. Smulders,et al.  Effect of electrical stimulation on veal quality. , 1986, Meat science.

[45]  Jianping Wang,et al.  Bactericidal effect of various non-thermal plasma agents and the influence of experimental conditions in microbial inactivation: A review , 2015 .

[46]  John H. Zhang,et al.  Cold Atmospheric Plasma: methods of production and application in dentistry and oncology , 2013, Medical gas research.

[47]  D. Graves,et al.  Ozone correlates with antibacterial effects from indirect air dielectric barrier discharge treatment of water , 2013 .

[48]  B. M. Watts,et al.  LIPID OXIDATION IN PRE-COOKED BEEF PRESERVED BY REFRIGERATION, FREEZING, AND IRRADIATION. , 1961 .

[49]  Gary C. Stevens,et al.  Surface modification of polymer surfaces: atmospheric plasma versus vacuum plasma treatments , 2001 .

[50]  Jianjun Shi,et al.  Physical Mechanisms of Inactivation of Bacillus subtilis Spores Using Cold Atmospheric Plasmas , 2006, IEEE Transactions on Plasma Science.

[51]  H. Barankova,et al.  Cold Atmospheric Plasma , 2008 .

[52]  M. Korachi,et al.  The Effect of Atmospheric Pressure Plasma Corona Discharge on pH, Lipid Content and DNA of Bacterial Cells , 2011 .

[53]  Muhlisin,et al.  Effect of Gaseous Ozone Exposure on the Bacteria Counts and Oxidative Properties of Ground Hanwoo Beef at Refrigeration Temperature , 2014, Korean journal for food science of animal resources.

[54]  Martin Clupek,et al.  Aqueous-phase chemistry and bactericidal effects from an air discharge plasma in contact with water: evidence for the formation of peroxynitrite through a pseudo-second-order post-discharge reaction of H2O2 and HNO2 , 2014 .

[55]  C. Faustman,et al.  Measurement of Discoloration in Fresh Meat , 2001 .

[56]  J. Kinsella,et al.  Initiation of lipid peroxidation in biological systems. , 1987, Critical reviews in food science and nutrition.

[57]  A. Jarmoluk,et al.  Effect of low-pressure plasma treatment on the color and oxidative stability of raw pork during refrigerated storage , 2016, Food science and technology international = Ciencia y tecnologia de los alimentos internacional.