Probing the Localized Surface Plasmon Field of a Gold Nanoparticle-Based Fibre Optic Biosensor

Gold nanoparticles (GNP) have been used in a variety of localized surface plasmon resonance (LSPR)-based optical sensor systems and in a variety of forms, such as colloidal suspensions, immobilized GNP on flat surfaces or optical fibres. A key parameter affecting the sensitivity of these systems is the effective depth of penetration of the surface plasmons. This study aims to determine the plasmon penetration depth in the case of an immobilized GNP-based LSPR optical biosensor. The optical biosensor used for experimentation is a U-bend fibre optic probe of 200-μm core diameter and 1.5-mm bend diameter on which GNP is immobilized. Formation of multilayered nanostructures on the immobilized GNP was used to investigate the field of the localized surface plasmons. Two multilayered nanostructures were explored in this study, viz. a polyelectrolyte multilayer formed by layer-by-layer (LBL) deposition of oppositely charged polyelectrolytes and an immunoglobulin G (IgG) multilayer formed through sequential immobilization of two mutually specific antibodies. Measurement of LSPR absorbance change with deposition of each analyte layer was used to determine the plasmon penetration depth (dP) of the LSPR biosensor. Probing the plasmon field with an IgG multilayer gave rise to at least twofold higher dP compared to dP obtained from the polyelectrolyte multilayer. The effect of GNP size was also studied, and GNP of three diameters, viz. 18, 36 and 45 nm, were used. The 36-nm-diameter GNP exhibited the highest dP. The outcomes of this study may provide leads for optimization of LSPR-based sensors for various biosensing applications.

[1]  T. Chinowsky,et al.  Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films , 1998 .

[2]  Soumyo Mukherji,et al.  Gold nanoparticle coated U-bend fibre optic probe for localized surface plasmon resonance based detection of explosive vapours , 2014 .

[3]  M. El-Sayed,et al.  Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response to size, shape, and metal composition. , 2006, The journal of physical chemistry. B.

[4]  S. Mukherji,et al.  Optimal Design for U-bent Fiber-optic LSPR Sensor Probes , 2014, Plasmonics.

[5]  Johannes Schmitt,et al.  Preparation and Optical Properties of Colloidal Gold Monolayers , 1999 .

[6]  J. Hafner,et al.  Localized surface plasmon resonance sensors. , 2011, Chemical reviews.

[7]  William L. Barnes,et al.  REVIEW ARTICLE: Surface plasmon polariton length scales: a route to sub-wavelength optics , 2006 .

[8]  William L Barnes,et al.  Surface plasmon – polariton length scales : a route to subwavelength optics , 2006 .

[9]  George C. Schatz,et al.  A nanoscale optical biosensor: The long range distance dependence of the localized surface plasmon resonance of noble metal nanoparticles , 2004 .

[10]  J. Hillier,et al.  A study of the nucleation and growth processes in the synthesis of colloidal gold , 1951 .

[11]  Heiko Ahrens,et al.  Influence of Adsorption Conditions on the Structure of Polyelectrolyte Multilayers , 2002 .

[12]  Adam,et al.  Determination of the spatial extension of the surface-plasmon evanescent field of a silver film with a photon scanning tunneling microscope. , 1993, Physical review. B, Condensed matter.

[13]  A. Vaskevich,et al.  Improved Sensitivity of Localized Surface Plasmon Resonance Transducers Using Reflection Measurements. , 2011, The journal of physical chemistry letters.

[14]  D. Davies,et al.  The three-dimensional structure at 6 A resolution of a human gamma Gl immunoglobulin molecule. , 1971, The Journal of biological chemistry.

[15]  George C Schatz,et al.  Localized surface plasmon resonance nanosensor: a high-resolution distance-dependence study using atomic layer deposition. , 2005, The journal of physical chemistry. B.

[16]  Sihai Chen,et al.  Plasmonic detection of a model analyte in serum by a gold nanorod sensor. , 2007, Analytical chemistry.

[17]  Limei Tian,et al.  Gold nanorods as plasmonic nanotransducers: distance-dependent refractive index sensitivity. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[18]  J. Homola,et al.  Optical sensors based on spectroscopy of localized surface plasmons on metallic nanoparticles: Sensitivity considerations , 2008, Biointerphases.

[19]  T Kobayashi,et al.  Local plasmon sensor with gold colloid monolayers deposited upon glass substrates. , 2000, Optics letters.

[20]  Thomas Read,et al.  Measurement of the localised plasmon penetration depth for gold nanoparticles using a non-invasive bio-stacking method. , 2013, Physical chemistry chemical physics : PCCP.

[21]  F. Rehfeldt,et al.  Swelling Behavior of Polyelectrolyte Multilayers in Saturated Water Vapor , 2004 .

[22]  R. V. Van Duyne,et al.  Localized surface plasmon resonance spectroscopy and sensing. , 2007, Annual review of physical chemistry.

[23]  R. W. Christy,et al.  Optical Constants of the Noble Metals , 1972 .

[24]  S. Mukherji,et al.  Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensor. , 2009, Biosensors & bioelectronics.

[25]  K. Lance Kelly,et al.  Chain Length Dependence and Sensing Capabilities of the Localized Surface Plasmon Resonance of Silver Nanoparticles Chemically Modified with Alkanethiol Self-Assembled Monolayers , 2001 .

[26]  Ashutosh Chilkoti,et al.  Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. , 2004, Analytical chemistry.

[27]  A. Vaskevich,et al.  Critical Issues in Localized Plasmon Sensing , 2014 .

[28]  Janos Vörös,et al.  The density and refractive index of adsorbing protein layers. , 2004, Biophysical journal.

[29]  P. Jain,et al.  Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. , 2006, The journal of physical chemistry. B.