The polymorphic human DNA sequence D8S8 assigned to 8ql3‐21.1, close to the carbonic anhydrase gene cluster, by isotopic and non‐isotopic in situ hybridization and by linkage analysis

Restriction fragment length polymorphism at the D8S8 locus is explained by the occurrence of at least two alternative alleles at two separate TaqI sites; Taql‐A allele frequencies 0–73 and 0–27 and Taql‐B allele frequencies 0–94 and 0–06. The D8S8 locus has been assigned to 8q 13–21.1, near to the carbonic anhydrase (CA) gene cluster, by in situ hybridization to metaphase chromosomes using both tritium and immunofluorescently labelled probes. Linkage analysis using the CEPH family DNA panel indicates a close genetic linkage between D8S8 and CA3, with a lod score of + 7–80 at TH – 0.05 in males.

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