Mechanism of transcriptional activation by Sp1: Evidence for coactivators

In reconstituted reactions, Sp1 stimulates transcription at TATA-containing promoters in the presence of semipurified TFIID fractions from either human or Drosophila cells, but is unable to do so when these fractions are replaced by purified, cloned Drosophila or yeast TFIID. Our findings with Sp1 and CTF suggest that partially purified TFIID fractions from human and Drosophila cells contain coactivators that are dispensable for basal transcription but are required as molecular adaptors between trans-activators and the general transcription initiation machinery. Experiments using cloned TFIID proteins suggest that these coactivators function through the amino-terminal portion of TFIID and that coactivator-TFIID interactions are species specific. At promoters lacking a TATA box, an additional activity distinct from coactivators is required for Sp1 activation of transcription.

[1]  Masami Horikoshi,et al.  Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex , 1988, Cell.

[2]  R. Tjian,et al.  Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis. , 1988, Science.

[3]  R. Tjian,et al.  Molecular mechanisms governing species-specific transcription of ribosomal RNA , 1989, Cell.

[4]  C. Kao,et al.  Yeast TATA-box transcription factor gene. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[5]  M. Horikoshi,et al.  Purification of a yeast TATA box-binding protein that exhibits human transcription factor IID activity. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[6]  P. Sharp,et al.  Five intermediate complexes in transcription initiation by RNA polymerase II , 1989, Cell.

[7]  P. Chambon,et al.  Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[8]  Nicolas Mermod,et al.  A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs , 1988, Nature.

[9]  P. Chambon,et al.  Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans‐acting factor. , 1984, The EMBO journal.

[10]  Robert Tjian,et al.  A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication , 1987, Cell.

[11]  P. Rigby,et al.  Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. , 1988, Genes & development.

[12]  Ira Herskowitz,et al.  A regulatory hierarchy for cell specialization in yeast , 1989, Nature.

[13]  D. Reinberg,et al.  Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE. , 1987, The Journal of biological chemistry.

[14]  D. Reinberg,et al.  Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site. , 1987, The Journal of biological chemistry.

[15]  R. Tjian,et al.  Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. , 1986, Science.

[16]  Steven Hahn,et al.  Isolation of the gene encoding the yeast TATA binding protein TFIID: A gene identical to the SPT15 suppressor of Ty element insertions , 1989, Cell.

[17]  J. Azizkhan,et al.  Functional analysis of GC element binding and transcription in the hamster dihydrofolate reductase gene promoter. , 1989, Nucleic acids research.

[18]  M. Horikoshi,et al.  Mechanism of action of a yeast activator: Direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions , 1988, Cell.

[19]  P. Sharp,et al.  Function of a yeast TATA element-binding protein in a mammalian transcription system , 1988, Nature.

[20]  A. Sentenac,et al.  A yeast activity can substitute for the HeLa cell TATA box factor , 1988, Nature.

[21]  R. Roeder,et al.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. , 1983, Nucleic acids research.

[22]  M. Ptashne.  How eukaryotic transcriptional activators work , 1988, Nature.

[23]  R. Tjian,et al.  Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. , 1989, Science.

[24]  R. Tjian,et al.  Distinct regions of Sp1 modulate DNA binding and transcriptional activation. , 1988, Science.

[25]  P. Chambon,et al.  Sequencing and expression of complementary DNA for the general transcription factor BTF3 , 1990, Nature.

[26]  Robert Tjian,et al.  Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain , 1987, Cell.

[27]  R. Roeder,et al.  Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region , 1985, Cell.

[28]  F. Winston,et al.  SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo , 1989, Cell.

[29]  D. Baltimore,et al.  The “initiator” as a transcription control element , 1989, Cell.

[30]  R. Tjian,et al.  Analysis of Sp1 in vivo reveals mutiple transcriptional domains, including a novel glutamine-rich activation motif , 1988, Cell.

[31]  A. Heguy,et al.  Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro , 1987, Cell.

[32]  W. Herr,et al.  The Oct-1 homoeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16 , 1989, Nature.

[33]  A. Saltzman,et al.  Promoter specificity and modulation of RNA polymerase II transcription , 1989, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[34]  Robert Tjian,et al.  Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro , 1985, Cell.

[35]  R. Lathe,et al.  Characterization of polyoma virus early proteins expressed from vaccinia virus recombinants. , 1988, Gene.

[36]  E. A. O'neill,et al.  The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain , 1989, Cell.

[37]  R. Roeder,et al.  Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[38]  J. Greenblatt,et al.  Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II , 1989, Nature.

[39]  S. Bell,et al.  The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF. , 1989, Genes & development.

[40]  R. Tjian,et al.  Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[41]  Masami Horikoshi,et al.  Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box , 1989, Nature.

[42]  Robert Tjian,et al.  Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID , 1990, Cell.

[43]  R. Tjian,et al.  Assembly of alternative multiprotein complexes directs rRNA promoter selectivity. , 1990, Genes & development.

[44]  M. Schmidt,et al.  Sp1 activates transcription without enhancing DNA-binding activity of the TATA box factor , 1989, Molecular and cellular biology.

[45]  M. Horikoshi,et al.  Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID , 1988, Molecular and cellular biology.