Immobilised enzymes: science or art?

Enzyme immobilisation is experiencing an important transition. Combinatorial approaches are increasingly applied in the design of robust immobilised enzymes by rational combination of fundamental immobilisation techniques (i.e. non-covalent adsorption, covalent binding, entrapment and encapsulation) or with other relevant technologies. The objective is to solve specific problems that cannot be solved by one of these basic immobilisation techniques.

[1]  Jyh-Ping Chen,et al.  Sol-gel powders and supported sol-gel polymers for immobilization of lipase in ester synthesis , 2003 .

[2]  C. Ortiz,et al.  Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters , 2002 .

[3]  R. Fernández-Lafuente,et al.  Enzymatic resolution of (±)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus oryzae via immobilization techniques , 2004 .

[4]  M. Arroyo,et al.  Stabilization of penicillin V acylase from Streptomyces lavendulae by covalent immobilization , 2001 .

[5]  Jinwon Park,et al.  Stability analysis of Bacillus stearothermophilus L1 lipase immobilized on surface-modified silica gels , 2004 .

[6]  S. W. Kim,et al.  Immobilization of glutaryl-7-aminocephalosporanic acid acylase on silica gel and enhancement of its stability , 2003, Applied biochemistry and biotechnology.

[7]  M. Hernáiz,et al.  Immobilization/stabilization on Eupergit C of the β-galactosidase from B. circulans and an α-galactosidase from Aspergillus oryzae , 2000 .

[8]  Jyh-Ping Chen,et al.  Synthesis of geranyl acetate by esterification with lipase entrapped in hybrid sol-gel formed within nonwoven fabric , 2002 .

[9]  R. Fernández-Lafuente,et al.  Strategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents , 1995 .

[10]  R. Fernández-Lafuente,et al.  Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins: Hydrolytic resolution of (R,S)-2-butyroyl-2-phenylacetic acid , 2003 .

[11]  X. Zhao,et al.  Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst , 2004 .

[12]  S. W. Kim,et al.  New Method for the Immobilization of Glutaryl-7-Aminocephalosporanic Acid Acylase on a Modified Epoxy/Silica Gel Hybrid. , 2002 .

[13]  Jian‐He Xu,et al.  Integration of purification with immobilization of Candida rugosa lipase for kinetic resolution of racemic ketoprofen. , 2004, Journal of biotechnology.

[14]  A. Anwar,et al.  Immobilization and stabilization of invertase on Cajanus cajan lectin support. , 2001, Bioresource technology.

[15]  M. Baboulene,,et al.  Regioselective bromohydroxylation of alkenes catalyzed by chloroperoxidase: Advantages of the immobilization of enzyme on talc , 1998 .

[16]  W. Hsu,et al.  Integrated enzyme purification and immobilization processes with immobilized metal affinity adsorbents , 2004 .

[17]  Nidal Hilal,et al.  Immobilization of cross-linked lipase aggregates within microporous polymeric membranes , 2004 .

[18]  R. Fernández-Lafuente,et al.  Prevention of interfacial inactivation of enzymes by coating the enzyme surface with dextran-aldehyde. , 2004, Journal of biotechnology.

[19]  M. Oka,et al.  Immobilization of bromelain onto porous copoly(γ-methyl-L-glutamate/L-leucine) beads , 2003 .

[20]  H. Bisswanger,et al.  Immobilization of trypsin on polyester fleece via different spacers , 2001 .

[21]  P. Halling,et al.  Preparation of new lipases derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization with polyethylenimine , 2001 .

[22]  E. Agostinelli,et al.  Immobilization of native and poly(ethylene glycol)‐treated (‘PEGylated’) bovine serum amine oxidase into a biocompatible hydrogel , 2001, Biotechnology and applied biochemistry.

[23]  J. Zink,et al.  Stabilization of Creatine Kinase Encapsulated in Silicate Sol-Gel Materials and Unusual Temperature Effects on Its Activity , 2002 .

[24]  Miguel Alcalde,et al.  Immobilization on Eupergit C of cyclodextrin glucosyltransferase (CGTase) and properties of the immobilized biocatalyst , 2003 .

[25]  L. Fischer,et al.  Improvement of enantioselectivity by immobilized imprinting of epoxide hydrolase from Rhodotorula glutinis , 2001 .

[26]  Jun Liu,et al.  Entrapping enzyme in a functionalized nanoporous support. , 2002, Journal of the American Chemical Society.

[27]  Roger A Sheldon,et al.  Immobilised enzymes: carrier-bound or carrier-free? , 2003, Current opinion in biotechnology.

[28]  J. Valentine,et al.  Molecular confinement influences protein structure and enhances thermal protein stability , 2001, Protein science : a publication of the Protein Society.

[29]  A. Pierre,et al.  Encapsulation of lipases in aerogels , 2001 .

[30]  S. W. Kim,et al.  Enhancement of stability of GL-7-ACA acylase immobilized on silica gel modified by epoxide silanization , 2003 .

[31]  J. O. Baker,et al.  Enhanced stability of glucoamylase through chemical crosslinking , 1989 .

[32]  K. Faber,et al.  Selectivity-enhancement in enantioselective hydrolysis of sec-alkyl sulfates by an alkylsulfatase from Rhodococcus ruber DSM 44541 , 2002 .

[33]  R. Fernández-Lafuente,et al.  Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase , 2002 .

[34]  A. Ghanem,et al.  Entrapment of Pseudomonas cepacia lipase with peracetylated β-cyclodextrin in sol–gel: application to the kinetic resolution of secondary alcohols , 2003 .

[35]  O. Abián,et al.  Co-aggregation of penicillin g acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media. , 2004, Biomacromolecules.

[36]  Uwe T Bornscheuer,et al.  Immobilizing enzymes: how to create more suitable biocatalysts. , 2003, Angewandte Chemie.

[37]  Jyh-Ping Chen,et al.  Polyvinyl formal resin plates impregnated with lipase-entrapped sol–gel polymer for flavor ester synthesis , 2003 .

[38]  H. Ijima,et al.  Effect of imprinting sol–gel immobilized lipase with chiral template substrates in esterification of (R)-(+)- and (S)-(−)-glycidol , 2002 .

[39]  Abraham Ulman,et al.  Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. , 2003, Journal of the American Chemical Society.

[40]  O. Abián,et al.  Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding enzyme molecules: New enzyme derivatives to be used in any reaction medium , 2002 .

[41]  I. Bakunina,et al.  A new precursor for the immobilization of enzymes inside sol-gel-derived hybrid silica nanocomposites containing polysaccharides. , 2004, Journal of biochemical and biophysical methods.

[42]  M. Reetz,et al.  Second Generation Sol‐Gel Encapsulated Lipases: Robust Heterogeneous Biocatalysts , 2003 .

[43]  R. Fernández-Lafuente,et al.  Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase. , 2001, Enzyme and microbial technology.

[44]  S. Mohapatra,et al.  Immobilization of α‐chymotrypsin for use in batch and continuous reactors , 2000 .

[45]  V. Torchilin,et al.  Stabilization of enzymes by intramolecular cross-linking using bifunctional reagents. , 1988, Methods in enzymology.

[46]  M. Arroyo,et al.  Covalent immobilization of penicillin acylase from Streptomyces lavendulae , 2000, Biotechnology Letters.

[47]  R. M. Blanco,et al.  Functionalization of mesoporous silica for lipase immobilization: Characterization of the support and the catalysts , 2004 .

[48]  R. Fernández-Lafuente,et al.  Preparation of a Stable Biocatalyst of Bovine Liver Catalase Using Immobilization and Postimmobilization Techniques , 2003, Biotechnology progress.

[49]  J. Dordick,et al.  Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (Subtilisin Carlsberg) , 2003 .