New Generation of Ultrasensitive Label-Free Optical Si Nanowire-Based Biosensors

We demonstrate the realization of the first label-free optical biosensor based on the room temperature luminescence of silicon nanowires (NWs) tested for the selective detection of C-reactive protein in human serum. High aspect ratio Si NW arrays used as sensing interface, are synthesized by a fast, low cost and Si industrially compatible approach. Si NW optical biosensors are fast and offer a broad concentration dynamic range that can be tuned according to different applications. Moreover, the platform is endowed with a high selectivity toward the target analyte and a sensitivity down to the fM limit of detection, opening the route toward noninvasive analysis in biofluids such as saliva.

[1]  M. J. Lo Faro,et al.  Light-emitting silicon nanowires obtained by metal-assisted chemical etching , 2017 .

[2]  M. Fornage,et al.  Heart Disease and Stroke Statistics—2017 Update: A Report From the American Heart Association , 2017, Circulation.

[3]  D. Wiersma,et al.  Coherent backscattering of Raman light , 2017, Nature Photonics.

[4]  Cristiano D'Andrea,et al.  Strongly enhanced light trapping in a two-dimensional silicon nanowire random fractal array , 2016, Light: Science & Applications.

[5]  M. J. Lo Faro,et al.  Silicon nanowire and carbon nanotube hybrid for room temperature multiwavelength light source , 2015, Scientific Reports.

[6]  Jeong-Woo Choi,et al.  Surface-enhanced Raman spectroscopy detection of dopamine by DNA Targeting amplification assay in Parkisons's model. , 2015, Biosensors & bioelectronics.

[7]  J. Salazar,et al.  C-Reactive Protein: Clinical and Epidemiological Perspectives , 2014, Cardiology research and practice.

[8]  L. Lazzarini,et al.  Visible and Infra-red Light Emission in Boron-Doped Wurtzite Silicon Nanowires , 2014, Scientific Reports.

[9]  T. Krauss,et al.  Silicon nanostructures for photonics and photovoltaics. , 2014, Nature nanotechnology.

[10]  S. Gan,et al.  Enzyme immunoassay and enzyme-linked immunosorbent assay. , 2013, The Journal of investigative dermatology.

[11]  Diana Gomes,et al.  Current analytical strategies for C-reactive protein quantification in blood. , 2013, Clinica chimica acta; international journal of clinical chemistry.

[12]  Tom Gregorkiewicz,et al.  Surface brightens up Si quantum dots: direct bandgap-like size-tunable emission , 2013, Light: Science & Applications.

[13]  Xingjiu Huang,et al.  CdSe quantum dots enhance electrical and electrochemical signals of nanogap devices for bioanalysis. , 2012, Small.

[14]  Judit Zsuga,et al.  The Hill equation and the origin of quantitative pharmacology , 2012 .

[15]  M. Galli,et al.  Quantum confinement and electroluminescence in ultrathin silicon nanowires fabricated by a maskless etching technique , 2012, Nanotechnology.

[16]  S. Prasad,et al.  CSF levels of oligomeric alpha-synuclein and beta-amyloid as biomarkers for neurodegenerative disease. , 2011, Integrative biology : quantitative biosciences from nano to macro.

[17]  K. Kostner,et al.  One-step homogeneous C-reactive protein assay for saliva. , 2011, Journal of immunological methods.

[18]  T. V. Duncan,et al.  Applications of nanotechnology in food packaging and food safety: Barrier materials, antimicrobials and sensors , 2011, Journal of Colloid and Interface Science.

[19]  Gengfeng Zheng,et al.  Nanowire biosensors for label-free, real-time, ultrasensitive protein detection. , 2011, Methods in molecular biology.

[20]  Gengfeng Zheng,et al.  Frequency domain detection of biomolecules using silicon nanowire biosensors. , 2010, Nano letters.

[21]  Zhongpin Zhang,et al.  Resonance energy transfer-amplifying fluorescence quenching at the surface of silica nanoparticles toward ultrasensitive detection of TNT. , 2008, Analytical chemistry.

[22]  James R Heath,et al.  Quantitative real-time measurements of DNA hybridization with alkylated nonoxidized silicon nanowires in electrolyte solution. , 2006, Journal of the American Chemical Society.

[23]  Gengfeng Zheng,et al.  Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species , 2006, Nature Protocols.

[24]  Igor L. Medintz,et al.  Biosensing with Luminescent Semiconductor Quantum Dots , 2006, Sensors (Basel, Switzerland).

[25]  R. Pereiro,et al.  The use of luminescent quantum dots for optical sensing , 2006 .

[26]  Ashok Mulchandani,et al.  Nanowire‐Based Electrochemical Biosensors , 2006 .

[27]  R. Lequin.  Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). , 2005, Clinical Chemistry.

[28]  Gengfeng Zheng,et al.  Multiplexed electrical detection of cancer markers with nanowire sensor arrays , 2005, Nature Biotechnology.

[29]  Sui-Lam Wong,et al.  Engineering Soluble Monomeric Streptavidin with Reversible Biotin Binding Capability* , 2005, Journal of Biological Chemistry.

[30]  Joseph Wang.  Carbon‐Nanotube Based Electrochemical Biosensors: A Review , 2005 .

[31]  Jin-Woo Choi,et al.  Disposable smart lab on a chip for point-of-care clinical diagnostics , 2004, Proceedings of the IEEE.

[32]  Kirk Martinez,et al.  Environmental sensor networks , 2004, Computer.

[33]  P. Alivisatos.  The use of nanocrystals in biological detection , 2004, Nature Biotechnology.

[34]  Itamar Willner,et al.  Probing photoelectrochemical processes in Au-CdS nanoparticle arrays by surface plasmon resonance: application for the detection of acetylcholine esterase inhibitors. , 2003, Journal of the American Chemical Society.

[35]  Weihong Tan,et al.  Ultrasensitive DNA detection using highly fluorescent bioconjugated nanoparticles. , 2003, Journal of the American Chemical Society.

[36]  P. Ridker.  Clinical application of C-reactive protein for cardiovascular disease detection and prevention. , 2003, Circulation.

[37]  Maria Miritello,et al.  Excitation and de-excitation properties of silicon quantum dots under electrical pumping , 2002 .

[38]  C. Lieber,et al.  Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species , 2001, Science.

[39]  Xiaogang Peng,et al.  Formation of high-quality CdTe, CdSe, and CdS nanocrystals using CdO as precursor. , 2001, Journal of the American Chemical Society.

[40]  I Lundström,et al.  Monitoring specific interaction of low molecular weight biomolecules on oxidized porous silicon using ellipsometry. , 1998, Biosensors & bioelectronics.

[41]  A. Alivisatos.  Semiconductor Clusters, Nanocrystals, and Quantum Dots , 1996, Science.

[42]  James H. Stathis,et al.  Luminescence degradation in porous silicon , 1992 .

[43]  M. Wilchek,et al.  Postsecretory modifications of streptavidin. , 1989, The Biochemical journal.