Title Lectins : production and practical applications

Lectins are proteins found in a diversity of organisms. They possess the ability to agglutinate erythrocytes with known carbohydrate specificity since they have at least one non-catalytic domain that binds reversibly to specific monosaccharides or oligosaccharides. This articles aims to review the production and practical applications of lectins. Lectins are isolated from their natural sources by chromatographic procedures or produced by recombinant DNA technology. The yields of animal lectins are usually low compared with the yields of plant lectins such as legume lectins. Lectins manifest a diversity of activities including antitumor, immunomo dulatory, antifungal, HIV-1 reverse transcriptase inhibitory, and anti-insect activities, which may find practical applications. A small number of lectins demonstrate antibacterial and anti-nematode activities.

[1]  T. Ng,et al.  First report of a haemagglutinin-induced apoptotic pathway in breast cancer cells. , 2010, Bioscience reports.

[2]  A. Karnchanatat,et al.  Antifungal and Antiproliferative Activities of Lectin from the Rhizomes of Curcuma amarissima Roscoe , 2010, Applied biochemistry and biotechnology.

[3]  J. Sun,et al.  First isolation and characterization of a novel lectin with potent antitumor activity from a Russula mushroom. , 2010, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[4]  S. Dalton,et al.  Glycoproteomic analysis of embryonic stem cells: identification of potential glycobiomarkers using lectin affinity chromatography of glycopeptides. , 2010, Journal of proteome research.

[5]  Y. Yokoyama,et al.  Purification, characterization and cDNA cloning of a novel lectin from the jellyfish Nemopilema nomurai. , 2010, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[6]  T. Ng,et al.  Isolation and characterization of a French bean hemagglutinin with antitumor, antifungal, and anti-HIV-1 reverse transcriptase activities and an exceptionally high yield. , 2010, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[7]  G. Ashraf,et al.  Purification, characterization, structural analysis and protein chemistry of a buffalo heart galectin-1 , 2010, Amino Acids.

[8]  K. Suguna,et al.  Purification, characterization and molecular cloning of a monocot mannose-binding lectin from Remusatia vivipara with nematicidal activity , 2010, Glycoconjugate Journal.

[9]  M. Taniwaki,et al.  Galectin-9 exhibits anti-myeloma activity through JNK and p38 MAP kinase pathways , 2010, Leukemia.

[10]  L. Migliolo,et al.  A lactose specific lectin from the sponge Cinachyrella apion: purification, characterization, N-terminal sequences alignment and agglutinating activity on Leishmania promastigotes. , 2010, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[11]  P. Verma,et al.  SUMO fusion facilitates expression and purification of garlic leaf lectin but modifies some of its properties. , 2010, Journal of biotechnology.

[12]  Sai-kun Pan,et al.  Isolation and characterization of a novel fucose-binding lectin from the gill of bighead carp (Aristichthys nobilis). , 2010, Veterinary immunology and immunopathology.

[13]  S. Tsao,et al.  A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. extralong autumn purple bean. , 2010, Journal of agricultural and food chemistry.

[14]  M. Swanson,et al.  A Lectin Isolated from Bananas Is a Potent Inhibitor of HIV Replication* , 2010, The Journal of Biological Chemistry.

[15]  G. Buck,et al.  Cell Sorting-Assisted Microarray Profiling of Host Cell Response to Cryptosporidium parvum Infection , 2009, Infection and Immunity.

[16]  E. Teixeira,et al.  Antifungal and marker effects of Talisia esculenta lectin on Microsporum canis in vitro , 2009, Journal of applied microbiology.

[17]  M. Kaur,et al.  Anti-insect potential of lectins from Arisaema species towards Bactrocera cucurbitae. , 2009, Journal of environmental biology.

[18]  T. Ng,et al.  Isolation and characterization of a lectin with potentially exploitable activities from caper (Capparis spinosa) seeds. , 2009, Bioscience reports.

[19]  Jiong Wu,et al.  PA‐MSHA inhibits proliferation and induces apoptosis through the up‐regulation and activation of caspases in the human breast cancer cell lines , 2009, Journal of cellular biochemistry.

[20]  J. Bao,et al.  Induction of apoptosis by Polygonatum odoratum lectin and its molecular mechanisms in murine fibrosarcoma L929 cells. , 2009, Biochimica et biophysica acta.

[21]  W. Ding,et al.  Insecticidal action of mammalian galectin-1 against diamondback moth (Plutella xylostella). , 2009, Pest management science.

[22]  T B Ng,et al.  A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa. , 2009, Acta biochimica Polonica.

[23]  B. Chatterjee,et al.  Purification and characterization of an N-acetylglucosamine specific lectin from marine bivalve Macoma birmanica. , 2009, Fish & shellfish immunology.

[24]  Lucília Domingues,et al.  cDNA Cloning and Functional Expression of the α-d-Galactose-Binding Lectin Frutalin in Escherichia coli , 2009, Molecular biotechnology.

[25]  A. Chaveerach,et al.  Dendrobium findleyanum agglutinin: production, localization, anti-fungal activity and gene characterization , 2009, Plant Cell Reports.

[26]  T. Ng,et al.  Musa acuminata (Del Monte banana) lectin is a fructose-binding lectin with cytokine-inducing activity. , 2009, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[27]  J. Hirabayashi,et al.  Mannose-binding lectin from yam (Dioscorea batatas) tubers with insecticidal properties against Helicoverpa armigera (Lepidoptera: Noctuidae). , 2009, Journal of agricultural and food chemistry.

[28]  B. Cavada,et al.  Larvicidal activity of lectins from Myracrodruon urundeuva on Aedes aegypti. , 2009, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[29]  J. Chen,et al.  A novel sialic acid-specific lectin from Phaseolus coccineus seeds with potent antineoplastic and antifungal activities. , 2009, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[30]  T. Ng,et al.  Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans) , 2009, Journal of biomedicine & biotechnology.

[31]  Hirofumi Hirai,et al.  Purification, Characterization, and cDNA Cloning of a Lectin from the Mushroom Pleurocybella porrigens , 2009, Bioscience, biotechnology, and biochemistry.

[32]  T. Ng,et al.  Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella. , 2009, Toxicon : official journal of the International Society on Toxinology.

[33]  M. Ghosh.  Purification of a lectin-like antifungal protein from the medicinal herb, Withania somnifera. , 2009, Fitoterapia.

[34]  J. Bao,et al.  Molecular mechanisms of Polygonatum cyrtonema lectin-induced apoptosis and autophagy in cancer cells , 2009, Autophagy.

[35]  M. R. Robles-Burgueño,et al.  Insecticidal action of PF2 lectin from Olneya tesota (Palo Fierro) against Zabrotes subfasciatus larvae and midgut glycoconjugate binding. , 2009, Journal of agricultural and food chemistry.

[36]  T. Ng,et al.  Novel galactonic acid-binding hexameric lectin from Hibiscus mutabilis seeds with antiproliferative and potent HIV-1 reverse transcriptase inhibitory activities. , 2009, Acta biochimica Polonica.

[37]  T. Cleveland,et al.  A maize lectin-like protein with antifungal activity against Aspergillus flavus. , 2009, Journal of food protection.

[38]  X. Ye,et al.  Purification of melibiose‐binding lectins from two cultivars of Chinese black soybeans , 2008, Acta biochimica et biophysica Sinica.

[39]  J. Carlsson,et al.  Lectin affinity chromatography as a tool to differentiate endogenous and recombinant erythropoietins. , 2008, Journal of chromatography. A.

[40]  J. Bao,et al.  A mannose-binding lectin from Sophora flavescens induces apoptosis in HeLa cells. , 2008, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[41]  A. Douglas,et al.  The insecticidal activity of recombinant garlic lectins towards aphids. , 2008, Insect biochemistry and molecular biology.

[42]  M. Wirth,et al.  Targeted drug delivery: binding and uptake of plant lectins using human 5637 bladder cancer cells. , 2008, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[43]  Lucília Domingues,et al.  Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris. , 2008, Protein expression and purification.

[44]  T. K. Maiti,et al.  Antitumor and proapoptotic effect of Abrus agglutinin derived peptide in Dalton's lymphoma tumor model. , 2008, Chemico-biological interactions.

[45]  M. I. Khan,et al.  Purification and characterization of a T-antigen specific lectin from the coelomic fluid of a marine invertebrate, sea cucumber (Holothuria scabra). , 2008, Fish & shellfish immunology.

[46]  J. Vishwanatha,et al.  Induction of mitochondria-dependent apoptosis by Abrus agglutinin derived peptides in human cervical cancer cell. , 2008, Toxicology in vitro : an international journal published in association with BIBRA.

[47]  M. Sahin,et al.  Cell surface glycosylation diversity of embryonic thymic tissues. , 2008, Acta histochemica.

[48]  T. Ng,et al.  Concurrent purification of two defense proteins from French bean seeds: a defensin‐like antifungal peptide and a hemagglutinin , 2007, Journal of peptide science : an official publication of the European Peptide Society.

[49]  B. Degnan,et al.  An ancient and variable mannose-binding lectin from the coral Acropora millepora binds both pathogens and symbionts. , 2008, Developmental and comparative immunology.

[50]  T. Ng,et al.  A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus. , 2008, Biochimica et biophysica acta.

[51]  E. Haubruge,et al.  Investigation of carbohydrate binding property of a fungal lectin from Xerocomus chrysenteron and potential use on Myzus persicae aphid. , 2008, Communications in agricultural and applied biological sciences.

[52]  G. Smagghe,et al.  Ectopically expressed leaf and bulb lectins from garlic (Allium sativum L.) protect transgenic tobacco plants against cotton leafworm (Spodoptera littoralis) , 2008, Transgenic Research.

[53]  S. Marangoni,et al.  Insecticidal action of Annona coriacea lectin against the flour moth Anagasta kuehniella and the rice moth Corcyra cephalonica (Lepidoptera: Pyralidae). , 2007, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[54]  E. Zenteno,et al.  Lectin from Phaseolus acutifolius var. escumite: chemical characterization, sugar specificity, and effect on human T-lymphocytes. , 2007, Journal of agricultural and food chemistry.

[55]  P. Proost,et al.  Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris. , 2007, Protein expression and purification.

[56]  R. H. Khan,et al.  Purification and Characterization of a Novel β-d-Galactosides-Specific Lectin from Clitoria ternatea , 2007, The protein journal.

[57]  M. L. Macedo,et al.  Insecticidal action of Bauhinia monandra leaf lectin (BmoLL) against Anagasta kuehniella (Lepidoptera: Pyralidae), Zabrotes subfasciatus and Callosobruchus maculatus (Coleoptera: Bruchidae). , 2007, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[58]  C. Pannecouque,et al.  Plant lectins are potent inhibitors of coronaviruses by interfering with two targets in the viral replication cycle , 2007, Antiviral Research.

[59]  S. Marangoni,et al.  Insecticidal and antifungal activity of a protein from Pouteria torta seeds with lectin-like properties. , 2007, Journal of agricultural and food chemistry.

[60]  O. Chernikov,et al.  A new lectin from the sea worm Serpula vermicularis: isolation, characterization and anti-HIV activity. , 2007, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[61]  T. Ng,et al.  A lectin with antifungal and mitogenic activities from red cluster pepper (Capsicum frutescens) seeds , 2007, Applied Microbiology and Biotechnology.

[62]  V. V. Velkov,et al.  Will transgenic plants adversely affect the environment? , 2005, Journal of Biosciences.

[63]  L. Mahal,et al.  Lectin microarrays for glycoprotein analysis. , 2007, Methods in molecular biology.

[64]  E. Haubruge,et al.  Effect of a fungal lectin from Xerocomus chrysenteron (XCL) on the biological parameters of aphids. , 2007, Communications in agricultural and applied biological sciences.

[65]  T. Ng,et al.  A hemagglutinin with mitogenic activity from dark red kidney beans. , 2006, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[66]  J. Singh,et al.  A novel antiproliferative and antifungal lectin from Amaranthus viridis Linn seeds. , 2006, Protein and peptide letters.

[67]  A. Saxena,et al.  A tuber lectin from Arisaema jacquemontii Blume with anti-insect and anti-proliferative properties. , 2006, Journal of biochemistry and molecular biology.

[68]  A. Gatehouse,et al.  Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion. , 2006, Journal of insect physiology.

[69]  T. Ng,et al.  An agglutinin with mitogenic and antiproliferative activities from the mushroom Flammulina velutipes. , 2006, Mycologia.

[70]  V. Semiglazov,et al.  Quality of life is improved in breast cancer patients by Standardised Mistletoe Extract PS76A2 during chemotherapy and follow-up: a randomised, placebo-controlled, double-blind, multicentre clinical trial. , 2006, Anticancer research.

[71]  E. De Clercq,et al.  Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua. , 2006, Acta biochimica et biophysica Sinica.

[72]  R. H. Khan,et al.  A tuber lectin from Arisaema helleborifolium Schott with anti-insect activity against melon fruit fly, Bactrocera cucurbitae (Coquillett) and anti-cancer effect on human cancer cell lines. , 2006, Archives of biochemistry and biophysics.

[73]  T. Ng,et al.  An antifungal protein from flageolet beans , 2005, Peptides.

[74]  Q. Yan,et al.  A novel homodimeric lectin from Astragalus mongholicus with antifungal activity. , 2005, Archives of biochemistry and biophysics.

[75]  Sampa Das,et al.  Insecticidal activity of Arum maculatum tuber lectin and its binding to the glycosylated insect gut receptors. , 2005, Journal of agricultural and food chemistry.

[76]  N. Benevides,et al.  Purification of a lectin from the marine red alga Gracilaria cornea and its effects on the cattle tick Boophilus microplus (Acari: Ixodidae). , 2005, Journal of agricultural and food chemistry.

[77]  N. Benevides,et al.  Purification of a lectin from the marine red alga Gracilaria ornata and its effect on the development of the cowpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae). , 2005, Biochimica et biophysica acta.

[78]  I. Goldstein,et al.  Cloning, expression in Escherichia coli and characterization of the recombinant Neu5Acalpha2,6Galbeta1,4GlcNAc-specific high-affinity lectin and its mutants from the mushroom Polyporus squamosus. , 2004, The Biochemical journal.

[79]  C. A. Andrade,et al.  Antitumor activity of Cratylia mollis lectin encapsulated into liposomes. , 2004, International journal of pharmaceutics.

[80]  S. Choi,et al.  Mistletoe lectin induces apoptosis and telomerase inhibition in human A253 cancer cells through dephosphorylation of akt , 2004, Archives of pharmacal research.

[81]  G. A. de Souza Filho,et al.  Expression and purification of the recombinant SALT lectin from rice (Oryza sativa L.). , 2004, Protein expression and purification.

[82]  S. Lyu,et al.  Preparation of alginate/chitosan microcapsules and enteric coated granules of mistletoe lectin , 2004, Archives of pharmacal research.

[83]  I. Goldstein,et al.  Hevein: an antifungal protein from rubber-tree (Hevea brasiliensis) latex , 2004, Planta.

[84]  R. H. Khan,et al.  Lectins as markers for blood grouping. , 2002, Medical science monitor : international medical journal of experimental and clinical research.

[85]  D. Sladić,et al.  A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity. , 2002, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[86]  S. Lyu,et al.  Inhibition of tumor growth and metastasis by Korean mistletoe lectin is associated with apoptosis and antiangiogenesis. , 2001, Cancer biotherapy & radiopharmaceuticals.

[87]  T. Ng,et al.  Isolation of a Homodimeric Lectin with Antifungal and Antiviral Activities from Red Kidney Bean (Phaseolus vulgaris) Seeds , 2001, Journal of protein chemistry.

[88]  S. Ho,et al.  Analysis of glycoconjugate patterns of normal and hormone‐induced dysplastic Noble rat prostates, and an androgen‐independent Noble rat prostate tumor, by lectin histochemistry and protein blotting , 2001, The Prostate.

[89]  P. Houterman,et al.  Processing, targeting, and antifungal activity of stinging nettle agglutinin in transgenic tobacco. , 1999, Plant physiology.

[90]  T. Ogawa,et al.  Functional and structural characterization of multiple galectins from the skin mucus of conger eel, Conger myriaster. , 1999, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[91]  T. Ng,et al.  Isolation and characterization of a mannose-binding lectin from leaves of the Chinese daffodil Narcissus tazetta. , 1998, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[92]  R. Adar,et al.  Synthesis of soybean agglutinin in bacterial and mammalian cells. , 1997, European journal of biochemistry.

[93]  W. Liu,et al.  Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum. , 2009, International journal of peptide and protein research.

[94]  Franky R. G. Terras,et al.  Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins. , 1992, Biochemistry.

[95]  J. Hansen,et al.  Correlation between carbohydrate structures on the envelope glycoprotein gp120 of HIV-1 and HIV-2 and syncytium inhibition with lectins. , 1989, AIDS.

[96]  W. Müller,et al.  The D-mannose-specific lectin from Gerardia savaglia blocks binding of human immunodeficiency virus type I to H9 cells and human lymphocytes in vitro. , 1988, Journal of acquired immune deficiency syndromes.

[97]  R. Dunn,et al.  Production of pea lectin in Escherichia coli. , 1986, The Journal of biological chemistry.

[98]  H. Rüdiger.  Purification and properties of blood-group-specific lectins from Vicia cracca. , 1977, European journal of biochemistry.

[99]  Y. Oshika,et al.  P-glycoprotein-mediated acquired multidrug resistance of human lung cancer cells in vivo. , 1996, British Journal of Cancer.

[100]  Haoyu,et al.  Enhancement of resistance to aphids by introducing the snowdrop lectin gene gna into maize plants , 2022 .