Tracer Tests and Image Analysis of Biological Clogging in a Two‐Dimensional Sandbox Experiment

A two-dimensional flow experiment on biological clogging was carried out by biostimulating a sandbox packed with sand inoculated with bacteria. Biostimulation consisted of continuously injecting nutrients (acetate and nitrate). Clogging was visualized by frequently carrying out colored tracer experiments using Brilliant Blue. The tracer experiments were recorded with a digital camera and converted to concentration maps using an image-analysis method that revealed in detail the complex spreading pattern surrounding clogged areas. Clogging resulted in a finger-like spreading of the tracer around the main clogged area. Fingers were asymmetric and their dominant direction changed over time. Although the flow field was complex around the main clogged area, the effect on the bulk hydraulic conductivity at the scale of the sandbox was very small.

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