Reduction of fibrinogen adsorption on PEG-coated polystyrene surfaces.

Reduction of protein adsorption by coating surfaces with polyethylene glycol (PEG) is well documented. The present work has four goals related to these previous studies: first, to develop chemistry providing densely packed, covalently bound PEG on polystyrene (PS); second, to determine the ability of these modified surfaces to reject fibrinogen; third, to compare the protein-rejecting ability of branched and linear PEGs; and fourth, to examine the utility of an ELISA-type procedure for measuring protein adsorption. It was found that PEG-epoxide could be readily coupled to amine groups of poly(ethylene imine) (PEI), which had been preadsorbed onto an oxidized PS surface. The PEG groups on branched PEGs appear to act as an excluded volume to repel proteins, similar to arguments previously raised for linear PEGs. The results of protein adsorption studies showed that fibrinogen adsorption is significantly reduced by coating polystyrene with either linear or branched PEGs of 1500 to 20,000 in molecular weight. The ELISA technique was found to be equivalent in sensitivity to radiolabeled fibrinogen for estimating adsorption levels. It is expected that PEG-coated PS will have much utility in a variety of biomedical applications.

[1]  J. H. Lee,et al.  Surface properties of copolymers of alkyl methacrylates with methoxy (polyethylene oxide) methacrylates and their application as protein-resistant coatings. , 1990, Biomaterials.

[2]  H. O. Senekjian,et al.  Gram Negative Peritonitis , 1983, The International journal of artificial organs.

[3]  B. Herren,et al.  Control of electroosmosis in coated quartz capillaries , 1987 .

[4]  G. Kenny,et al.  Principles, problems, and strategies in the use of antigenic mixtures for the enzyme-linked immunosorbent assay , 1983, Journal of clinical microbiology.

[5]  J. Feijen,et al.  Poly(dimethylsiloxane)-poly(ethylene oxide)-heparin block copolymers. II: Surface characterization and in vitro assessments. , 1990, Journal of biomedical materials research.

[6]  J. M. Harris,et al.  Assessment of the Effects of Attaching an Enzyme to Glass by a Poly(ethylene glycol) Tether , 1989 .

[7]  S. Cooper,et al.  Bulk, surface, and blood-contacting properties of polyetherurethanes modified with polyethylene oxide. , 1989, Journal of biomaterials science. Polymer edition.

[8]  A. Turner,et al.  Ferrocene-mediated enzyme electrode for amperometric determination of glucose. , 1984, Analytical chemistry.

[9]  W. Bale,et al.  High specific activity iodination of gamma-globulin with iodine-131 monochloride. , 1960, Cancer research.

[10]  K. Holmberg,et al.  Covalent immobilization of lipase in organic solvents , 1989, Biotechnology and bioengineering.

[11]  T. Horbett.  Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. II. Compositional analysis with the prelabeled protein technique. , 1981, Journal of biomedical materials research.

[12]  J. Eriksson,et al.  Characterization of kmno4/h2so4-oxidized polyethylene surfaces by means of ESCA and45ca2+ adsorption , 1984 .

[13]  A. Mcfarlane,et al.  Efficient Trace-labelling of Proteins with Iodine , 1958, Nature.

[14]  J. Feijen,et al.  Interaction of antithrombin III with preadsorbed albumin-heparin conjugates. , 1984, Biomaterials.

[15]  É. Kiss,et al.  Surface grafting of polyethyleneoxide optimized by means of ESCA , 1987 .

[16]  S. Nagaoka,et al.  Interaction Between Blood Components and Hydrogels With Poly(Oxyethylene) Chains , 1984 .

[17]  A. Gardas,et al.  Coating of proteins to polystyrene ELISA plates in the presence of detergents. , 1988, Journal of immunological methods.

[18]  C. Gölander,et al.  ESCA studies of the adsorption of polyethyleneimine and glutaraldehyde-reacted polyethyleneimine on polyethylene and mica surfaces , 1987 .

[19]  J. M. Harris,et al.  Proton NMR characterization of poly(ethylene glycols) and derivatives , 1990 .

[20]  R. Jay.  Direct Titration of Epoxy Compounds and Aziridines. , 1964 .