Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs

The Xenopus laevis alpha 1- and beta 1-globin genes were injected into oocytes and unfertilized eggs of X. laevis. In oocytes, the injected globin genes were actively transcribed, but the majority of the transcripts were incorrectly initiated. In unfertilized eggs, the injected genes were transcribed at a low level but only from the correct start sites. In oocytes, the injected circular plasmid DNA containing the cloned globin genes persisted but did not replicate. In contrast, DNA injected into unfertilized eggs replicated up to 15-fold within a 22-h period. We suggest that the ability of the egg to selectively transcribe the injected X. laevis globin genes from the correct promoter sites may be related to differences in chromatin structure between the oocyte and the unfertilized egg.

[1]  A. Worcel,et al.  Chromatin assembly in Xenopus oocytes: In vivo studies , 1984, Cell.

[2]  J. Williams,et al.  Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs , 1984, Molecular and cellular biology.

[3]  D. L. Weeks,et al.  Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes , 1983, Molecular and cellular biology.

[4]  J. Williams,et al.  Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[5]  H. Weintraub,et al.  Transcription of DNA injected into Xenopus oocytes is influenced by template topology , 1983, Nature.

[6]  R. Harland,et al.  Induction of chromosome replication during maturation of amphibian oocytes. , 1983, Ciba Foundation symposium.

[7]  G. Gargiulo,et al.  Properties of the chromatin assembled on DNA injected into Xenopus oocytes and eggs. , 1983, Cold Spring Harbor symposia on quantitative biology.

[8]  J. Mertz,et al.  Template Structural Requirements for Transcription In Vivo by RNA Polymerase II , 1982, Molecular and cellular biology.

[9]  L. J. Korn,et al.  Oocyte extracts reactivate developmentally inert Xenopus 5S genes in somatic nuclei , 1982, Nature.

[10]  M. Kirschner,et al.  A major developmental transition in early xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage , 1982, Cell.

[11]  M. Kirschner,et al.  A major developmental transition in early xenopus embryos: II. control of the onset of transcription , 1982, Cell.

[12]  R. Harland,et al.  DNA synthesis in a cell-free system from Xenopus eggs: Priming and elongation on single-stranded DNA in vitro , 1982, Cell.

[13]  R. Reeder,et al.  Fate of amplified nucleoli in Xenopus laevis embryos. , 1982, Developmental biology.

[14]  S. Orkin.  Organization and expression of globin genes , 1982 .

[15]  R. Axel,et al.  Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: Identification of an upstream control region , 1981, Cell.

[16]  M. Bendig.  Persistence and expression of histone genes injected into Xenopus eggs in early development , 1981, Nature.

[17]  P. Matthias,et al.  Transcription of Complex Structural Genes in the Xenopus Oocyte System , 1981 .

[18]  D. Melton,et al.  Gene transfer in amphibian eggs and oocytes. , 1981, Annual review of genetics.

[19]  R. Harland,et al.  Regulated replication of DNA microinjected into eggs of Xenopus laevis , 1980, Cell.

[20]  Jeffrey G. Williams,et al.  Internal organization of the major adult α- and β-globin genes of X. laevis , 1980, Cell.

[21]  A. Jeffreys,et al.  Linkage of adult α- and β-globin genes in X. laevis and gene duplication by tetraploidization , 1980, Cell.

[22]  M. Wickens,et al.  Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei , 1980, Nature.

[23]  R. Maxson,et al.  The synthesis of authentic sea urchin transcriptional and translational products by sea urchin histone genes injected into Xenopus laevis oocytes. , 1980, Developmental biology.

[24]  D. Sherratt,et al.  Trans-complementable copy-number mutants of plasmid ColE1 , 1980, Nature.

[25]  M. Birnstiel,et al.  Surrogate Genetics in the Frog Oocyte , 1980 .

[26]  A. Graessmann,et al.  Transfer of Cell Constituents into Eukaryotic Cells , 1980, NATO Advanced Study Institutes Series.

[27]  T. Taguchi,et al.  Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α , 1978, Nature.

[28]  J. Finch,et al.  Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes. , 1978, Developmental biology.

[29]  J. Mertz,et al.  Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis. , 1978, Cold Spring Harbor symposia on quantitative biology.

[30]  J. Mertz,et al.  Purified DNAs are transcribed after microinjection into Xenopus oocytes. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[31]  H. Woodland,et al.  DNA synthesis in ocytes and eggs of Xenopus laevis injected with DNA. , 1975, Developmental biology.

[32]  J. Gurdon.  The control of gene expression in animal development , 1974 .

[33]  F. Billett,et al.  Practical Studies of Animal Development , 1974 .

[34]  J. Gurdon,et al.  Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm. , 1973, European journal of biochemistry.

[35]  R. Wallace,et al.  Protein incorporation by isolated amphibian oocytes III. Optimum incubation conditions , 1973 .

[36]  J. Gurdon,et al.  The replication of purified DNA introduced into living egg cytoplasm. , 1969, Biochimica et biophysica acta.

[37]  S. Rusconi,et al.  Transformation of frog embryos with a rabbit fB-globin gene , 2022 .