Coherent anti-Stokes Raman scattering microscopy using photonic crystal fibers

The performance of two different photonic crystal fibers (PCF) of identical lengths for implementation of the Stokes source in a multimodal CARS microscopy and spectroscopy setup is compared. RIN measurements are performed to experimentally determine the noise in the supercontinuum from the two fibers as well as in the CARS signal under similar excitation conditions of the input pulse into the PCF. The RIN of the CARS signal is found to be higher than the RIN of the corresponding Stokes signal, in both fibers. The implications for CARS microscopy of the SC spectrum and its noise dependence on input pulse conditions in both fibers, are discussed.