The effect of an intronic polymorphism on alcohol dehydrogenase expression in Drosophila melanogaster.

Several lines of evidence indicate that natural selection controls the frequencies of an allozyme polymorphism at the alcohol dehydrogenase (Adh) locus in Drosophila melanogaster. However, because of associations among sequence polymorphisms in the Adh region, it is not clear whether selection acts directly (or solely) on the allozymic site. This problem has been approached by using in vitro mutagenesis to distinguish among the effects on Adh expression of individual polymorphisms. This study shows that a polymorphism within the first Adh intron (delta 1) has a significant effect on the level of ADH protein. Like the allozyme, delta 1 shows a geographic cline in frequency, indicating that it may also be a target of natural selection. These results suggest that multisite selection models may be required to understand the evolutionary dynamics of individual loci.

[1]  M. Kreitman,et al.  Molecular analysis of an allozyme cline: alcohol dehydrogenase in Drosophila melanogaster on the east coast of North America. , 1993, Genetics.

[2]  V. Bénassi,et al.  Molecular variation of Adh and P6 genes in an African population of Drosophila melanogaster and its relation to chromosomal inversions. , 1993, Genetics.

[3]  J. D. Huang,et al.  The interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression. , 1993, Genes & development.

[4]  S. Freytag,et al.  Contribution of specific cis-acting elements to activity of the mouse pro-alpha 2(I) collagen enhancer. , 1993, The Journal of biological chemistry.

[5]  R. Brasseur,et al.  Two amino acid substitutions in apolipoprotein B are in complete allelic association with the antigen group (x/y) polymorphism: evidence for little recombination in the 3' end of the human gene. , 1992, American journal of human genetics.

[6]  D. Shoemaker,et al.  High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. , 1991, Gene.

[7]  C. Laurie,et al.  Use of in vitro mutagenesis to analyze the molecular basis of the difference in Adh expression associated with the allozyme polymorphism in Drosophila melanogaster. , 1991, Genetics.

[8]  C. Laurie,et al.  Associations between DNA sequence variation and variation in expression of the Adh gene in natural populations of Drosophila melanogaster. , 1991, Genetics.

[9]  R. Hudson,et al.  Inferring the evolutionary histories of the Adh and Adh-dup loci in Drosophila melanogaster from patterns of polymorphism and divergence. , 1991, Genetics.

[10]  A. Jacobson,et al.  Tales of poly(A): a review. , 1990, Gene.

[11]  T. Maniatis,et al.  Identification of cis-regulatory elements required for larval expression of the Drosophila melanogaster alcohol dehydrogenase gene. , 1990, Genetics.

[12]  C. Gorman,et al.  Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA. , 1990, Nucleic acids research.

[13]  T. Maniatis,et al.  The role of specific enhancer-promoter interactions in the Drosophila Adh promoter switch. , 1989, Genes & development.

[14]  B. Aronow,et al.  Evidence for a complex regulatory array in the first intron of the human adenosine deaminase gene. , 1989, Genes & development.

[15]  R. Renkawitz-Pohl,et al.  Intron and upstream sequences regulate expression of the Drosophila beta 3-tubulin gene in the visceral and somatic musculature, respectively. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. Gibson,et al.  Genetic differentiation in populations of Drosophila melanogaster from the Peoples' Republic of China: comparison with patterns on other continents , 1989, Heredity.

[17]  M. Kreitman,et al.  MOLECULAR ANALYSIS OF THE ALLELES OF ALCOHOL DEHYDROGENASE ALONG A CLINE IN DROSOPHILA MELANOGASTER. I. MAINE, NORTH CAROLINA, AND FLORIDA , 1989, Evolution; international journal of organic evolution.

[18]  J. David,et al.  Latitudinal variation of Adh gene frequencies in Drosophila melanogaster: a Mediterranean instability , 1989, Heredity.

[19]  A. Buchman,et al.  Comparison of intron-dependent and intron-independent gene expression , 1988, Molecular and cellular biology.

[20]  C. Laurie,et al.  Quantitative analysis of RNA produced by slow and fast alleles of Adh in Drosophila melanogaster. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[21]  M. Aguadé Restriction map variation at the adh locus of Drosophila melanogaster in inverted and noninverted chromosomes. , 1988, Genetics.

[22]  C. C. Laurie-Ahlberg,et al.  Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. , 1987, Genetics.

[23]  C. Aquadro,et al.  Molecular population genetics of the alcohol dehydrogenase gene region of Drosophila melanogaster. , 1986, Genetics.

[24]  M. Aguadé,et al.  Genetic uniformity in two populations of Drosophila melanogaster as revealed by filter hybridization of four-nucleotide-recognizing restriction enzyme digests. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[25]  M. Ashburner,et al.  The expression of the gene coding for alcohol dehydrogenase during the development of Drosophila melanogaster , 1986 .

[26]  P. K. Smith,et al.  Measurement of protein using bicinchoninic acid. , 1985, Analytical biochemistry.

[27]  C. C. Laurie-Ahlberg Genetic variation affecting the expression of enzyme-coding genes in Drosophila: an evolutionary perspective. , 1985, Isozymes.

[28]  T. Maniatis,et al.  Identification of DNA sequences required for the regulation of Drosophila alcohol dehydrogenase gene expression. , 1985, Cold Spring Harbor symposia on quantitative biology.

[29]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[30]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[31]  M. Ashburner,et al.  The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5′ end in different developmental stages , 1983, Cell.

[32]  J. Oakeshott,et al.  ALCOHOL DEHYDROGENASE AND GLYCEROL‐3‐PHOSPHATE DEHYDROGENASE CLINES IN DROSOPHILA MELANOGASTER ON DIFFERENT CONTINENTS , 1982, Evolution; international journal of organic evolution.

[33]  W. Delden The Alcohol Dehydrogenase Polymorphism in Drosophila melanogaster , 1982 .