Isolation and characterization of fibronectin from bovine aqueous humor.

Aqueous humor collected from freshly enucleated bovine eyes was passed over a gelatin affinity column, and bound material was eluted with 4M urea. When fractionated by sodium dodecyl sulfate electrophoresis, the eluted material co-migrated with human plasma fibronectin and protein standards of approximately 220,000 daltons. Amino acid analyses demonstrated a composition similar to that of human plasma fibronectin and fibronectin synthesized by cultured cells. The concentration of fibronectin in aqueous humor is approximately 100 times less than in human plasma.

[1]  E. Ruoslahti,et al.  Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix , 1979, The Journal of cell biology.

[2]  J. Rosenbloom,et al.  Fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts , 1979, Cell.

[3]  Kenneth M. Yamada,et al.  The influence of an adhesive cell surface protein on chondrogenic expression in vitro. , 1979, Experimental cell research.

[4]  P. Bornstein,et al.  Isolation of a collagen-binding fragment from fibronectin and cold-insoluble globulin. , 1979, The Journal of biological chemistry.

[5]  K. Yamada,et al.  Fibroblast cellular and plasma fibronectins are similar but not identical , 1979, The Journal of cell biology.

[6]  B. A. Bray.  Cold-insoluble globulin (fibronectin) in connective tissues of adult human lung and in trophoblast basement membrane. , 1978, The Journal of clinical investigation.

[7]  Kenneth M. Yamada,et al.  Fibronectins—adhesive glycoproteins of cell surface and blood , 1978, Nature.

[8]  D. Gospodarowicz,et al.  ROLE OF THE HIGH‐MOLECULAR‐WEIGHT GLYCOPROTEIN IN CELLULAR MORPHOLOGY, ADHESION, AND DIFFERENTIATION * , 1978, Annals of the New York Academy of Sciences.

[9]  J. McDougall,et al.  MODULATION OF CELL SURFACE GLYCOCALYX: STUDIES ON LARGE, EXTERNAL, TRANSFORMATION‐SENSITIVE PROTEIN * , 1978, Annals of the New York Academy of Sciences.

[10]  I. Pastan,et al.  TRANSFORMATION‐SENSITIVE CELL SURFACE PROTEIN: ISOLATION, CHARACTERIZATION, AND ROLE IN CELLULAR MORPHOLOGY AND ADHESION , 1978, Annals of the New York Academy of Sciences.

[11]  A. Vaheri,et al.  Distribution of a major connective tissue protein, fibronectin, in normal human tissues , 1978, The Journal of experimental medicine.

[12]  Mosesson Mw.  Cold-insoluble globulin (CIg), a circulating cell surface protein. , 1977 .

[13]  E. Engvall,et al.  Binding of soluble form of fibroblast surface protein, fibronectin, to collagen , 1977, International journal of cancer.

[14]  M. Bernfield,et al.  Basal lamina of embryonic salivary epithelia. Production by the epithelium and role in maintaining lobular morphology , 1977, The Journal of cell biology.

[15]  P. Benya,et al.  The progeny of rabbit articular chondrocytes synthesize collagen types I and III and type I trimer, but not type II. Verifications by cyanogen bromide peptide analysis. , 1977, Biochemistry.

[16]  E. Ruoslahti,et al.  Distribution of fibroblast surface antigen in the developing chick embryo , 1975, The Journal of experimental medicine.

[17]  K. Yamada,et al.  Isolation of a major cell surface glycoprotein from fibroblasts. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[18]  E. Hartree,et al.  Determination of protein: a modification of the Lowry method that gives a linear photometric response. , 1972, Analytical biochemistry.

[19]  Kenneth M. Yamada,et al.  Cell Surface Protein and Cell Interactions , 1980 .

[20]  D. Mosher,et al.  High molecular weight, cell surface-associated glycoprotein (fibronectin) lost in malignant transformation. , 1978, Biochimica et biophysica acta.

[21]  M. Mosesson.  Cold-insoluble globulin (CIg), a circulating cell surface protein. , 1977, Thrombosis and haemostasis.

[22]  A. Veis,et al.  The acidic glycosaminoglycans in human fetal development and adult life: Cornea, sclera and skin , 1972 .