论文信息 - CCD video observation of microgravity crystallization: apocrustacyanin C1

CCD video observation of microgravity crystallization: apocrustacyanin C1

Abstract Apocrustacyanin C 1 has been crystallized in the vapour-diffusion apparatus of ESA's Advanced Protein Crystallization Facility (APCF) on-board the NASA space shuttle STS-65 International Microgravity Laboratory-2 (IML-2) mission. Crystal growth was monitored by black and white CCD observation at time intervals throughout the experiment. The resulting crystals displayed a motion within the hanging drop that is attributed to Marangoni convection effects. The images also show a “halo” effect around the growing crystals which can be attributed to the presence of depletion zones i.e. solution regions which are depleted of this coloured protein.

[1] J. Stapelmann,et al. Experiment equipment for protein crystallization in μg facilities , 1992 .

[2] G. G. Stokes. "J." , 1890, The New Yale Book of Quotations.

[3] A. McPherson,et al. Protein and virus crystal growth on international microgravity laboratory-2. , 1995, Biophysical journal.

[4] Robert S. Snyder,et al. Protein crystallization facilities for microgravity experiments , 1991 .

[5] F. Lefaucheux,et al. Crystal growth in gels: Principle and applications , 1988 .

[6] G. Feher,et al. On the crystallization of proteins. , 1978, Journal of molecular biology.

[7] R S Snyder,et al. Protein crystal growth. Growth kinetics for tetragonal lysozyme crystals. , 1986, The Journal of biological chemistry.

[8] P. F. Zagalsky,et al. Complete sequence and model for the C1 subunit of the carotenoprotein, crustacyanin, and model for the dimer, beta-crustacyanin, formed from the C1 and A2 subunits with astaxanthin. , 1991, European journal of biochemistry.