Enhancement of Immune Activities of Natural Water-Soluble Sulforaphane by Nano Encapsulation Process

ABSTRACT : This study was performed to investigate improving immune activities of natural water-soluble sulforaphaneextracted from Brassica oleracea var. italica by nano encapsulation process. The nano particles of the sulforaphane extractedwith ultrasonification process at 60 promoted human B and T cell growth, about 7~35% compared to the control. Thesecretion of IL-6 and TNF- α from T cells were also enhanced as 2.6 × 10 − 4 pg/cell and 2.1 × 10 4 pg/cell, respectively, by theadding nano samples. NK cell activation was improved about 8%, compare to the control in adding cultured medium of Tcell added nano samples. It was also found that sulforaphane extracted from B. oleracea var. italica had highly inhibitoryactivity on hyaluronidase as IC 50 about 200 / . It can be concluded that natural water-soluble sulforaphane samples bynano-encapsulation, each size is 200 , extracted from B. oleracea var. italica has high immune activities through higherefficiency of bio-activation than conventional extracts.

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