Insight into E-selectin/ligand interaction from the crystal structure and mutagenesis of the lec/EGF domains

The three-dimensional structure of the ligand-binding region of human E-selectin has been determined at 2.0 Å resolution. The structure reveals limited contact between the two domains and a coordination of Ca2+ not predicted from other C-type lectins. Structure/function analysis indicates a defined region and specific amino-acid side chains that may be involved in ligand binding. These features of the E-selectin/ligand interaction have important implications for understanding the recruitment of leukocytes to sites of inflammation.

[1]  W. Weis,et al.  Structure of the calcium-dependent lectin domain from a rat mannose-binding protein determined by MAD phasing. , 1991, Science.

[2]  T. A. Jones,et al.  A graphics model building and refinement system for macromolecules , 1978 .

[3]  E. Butcher.  Leukocyte-endothelial cell recognition: Three (or more) steps to specificity and diversity , 1991, Cell.

[4]  Wayne A. Hendrickson,et al.  Structure of a C-type mannose-binding protein complexed with an oligosaccharide , 1992, Nature.

[5]  L. Lasky,et al.  Selectins: interpreters of cell-specific carbohydrate information during inflammation. , 1992, Science.

[6]  I. Weissman,et al.  Human homologue of mouse lymph node homing receptor: evolutionary conservation at tandem cell interaction domains. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[7]  I. Weissman,et al.  Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains. , 1989, Science.

[8]  L. Lasky,et al.  The Mel 14 antibody binds to the lectin domain of the murine peripheral lymph node homing receptor , 1990, The Journal of cell biology.

[9]  R. Huber,et al.  Structure of human des(1-45) factor Xa at 2.2 A resolution. , 1993, Journal of molecular biology.

[10]  D. Vestweber,et al.  Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells , 1993, The Journal of cell biology.

[11]  L. Presta,et al.  P- and E-selectin use common sites for carbohydrate ligand recognition and cell adhesion , 1993, The Journal of cell biology.

[12]  M. Karplus,et al.  Crystallographic R Factor Refinement by Molecular Dynamics , 1987, Science.

[13]  F. Gaeta,et al.  Ligand recognition by E-selectin : analysis of conformation and activity of synthetic monomeric and bivalent sialyl Lewis X analogs , 1993 .

[14]  C. Bush,et al.  Solution structure of the Lewis x oligosaccharide determined by NMR spectroscopy and molecular dynamics simulations. , 1992, Biochemistry.

[15]  M. Becker‐André,et al.  Murine endothelial leukocyte-adhesion molecule 1 is a close structural and functional homologue of the human protein. , 1992, European journal of biochemistry.

[16]  S. Nagata,et al.  pEF-BOS, a powerful mammalian expression vector. , 1990, Nucleic acids research.

[17]  M. Taylor,et al.  Evolutionary conservation of intron position in a subfamily of genes encoding carbohydrate-recognition domains. , 1991, The Journal of biological chemistry.

[18]  J. Bajorath,et al.  Interaction of P-selectin (CD62) and its cellular ligand: analysis of critical residues. , 1993, Biochemistry.

[19]  I. Campbell,et al.  The three‐dimensional structure of the first EGF‐like module of human factor IX: Comparison with EGF and TGF‐α , 1992, Protein science : a publication of the Protein Society.

[20]  A. Brunger.  Free R value: a novel statistical quantity for assessing the accuracy of crystal structures. , 1992 .

[21]  D. Vestweber,et al.  Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. , 1992, The Journal of biological chemistry.

[22]  B. Seed,et al.  Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. , 1990, Science.

[23]  W. Kabsch,et al.  Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical features , 1983, Biopolymers.

[24]  R. Cummings,et al.  Characterization of a specific ligand for P-selectin on myeloid cells. A minor glycoprotein with sialylated O-linked oligosaccharides. , 1993, The Journal of biological chemistry.

[25]  R. Cotran,et al.  Identification of an inducible endothelial-leukocyte adhesion molecule. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[26]  M. Bednarski,et al.  Synthesis and structural analysis using 2-D NMR of sialyl Lewis X (SLex) and Lewis X (Lex) oligosaccharides: ligands related to E-selectin [ELAM-1] binding. , 1992 .

[27]  H. Scheraga,et al.  Solution structure of murine epidermal growth factor determined by NMR spectroscopy and refined by energy minimization with restraints. , 1994, Biochemistry.

[28]  D. Kohda,et al.  Polypeptide chain fold of human transforming growth factor alpha analogous to those of mouse and human epidermal growth factors as studied by two-dimensional 1H NMR. , 1989, Biochemistry.

[29]  R. Dwek,et al.  The solution conformation of the Leχ group , 1991 .

[30]  D. Dowbenko,et al.  Cloning of a lymphocyte homing receptor reveals a lectin domain , 1989, Cell.

[31]  L. Presta,et al.  Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion [published erratum appears in J Cell Biol 1993 Feb;120(4):1071] , 1992, The Journal of cell biology.

[32]  R. Cummings,et al.  Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells , 1992, The Journal of cell biology.

[33]  Thomas A. Kunkel,et al.  Rapid and efficient site-specific mutagenesis without phenotypic selection. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[34]  S. Watson,et al.  The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide , 1992, The Journal of cell biology.

[35]  T. C. Tsang,et al.  Characterization of cDNA and genomic sequences encoding rabbit ELAM-1: conservation of structure and functional interactions with leukocytes. , 1992, DNA and cell biology.

[36]  Chi‐Huey Wong,et al.  Conformational Studies of Sialyl Lewis-X in Aqueous-Solution , 1992 .

[37]  J M Thornton,et al.  Pi-pi interactions: the geometry and energetics of phenylalanine-phenylalanine interactions in proteins. , 1991, Journal of molecular biology.

[38]  B. Seed,et al.  Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. , 1989, Science.

[39]  K. Drickamer,et al.  Role of conserved and nonconserved residues in the Ca(2+)-dependent carbohydrate-recognition domain of a rat mannose-binding protein. Analysis by random cassette mutagenesis. , 1992, The Journal of biological chemistry.

[40]  M P Bevilacqua,et al.  Endothelial-leukocyte adhesion molecules. , 1993, Annual review of immunology.

[41]  M. Ullner,et al.  How an epidermal growth factor (EGF)-like domain binds calcium. High resolution NMR structure of the calcium form of the NH2-terminal EGF-like domain in coagulation factor X. , 1994, The Journal of biological chemistry.

[42]  R. Cook,et al.  Cloning of GMP-140, a granule membrane protein of platelets and endothelium: Sequence similarity to proteins involved in cell adhesion and inflammation , 1989, Cell.

[43]  S. Brown,et al.  Solution structures of human transforming growth factor alpha derived from 1H NMR data. , 1990, Biochemistry.

[44]  I. Campbell,et al.  The solution structure of human transforming growth factor alpha. , 1993, European journal of biochemistry.

[45]  S. Watson,et al.  An endothelial ligand for L-Selectin is a novel mucin-like molecule , 1992, Cell.

[46]  S Goelz,et al.  Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[47]  I. Campbell,et al.  Human epidermal growth factor , 1992 .

[48]  T. Tedder,et al.  Isolation and characterization of a bovine cDNA encoding a functional homolog of human P-selectin. , 1993, Biochemical and Biophysical Research Communications - BBRC.