Development and characterization of monoclonal antibodies against excretory-secretory antigens of Fasciola gigantica.

Monoclonal antibodies (MAbs) directed against Fasciola gigantica excretory-secretory (ES) antigens were developed from BALB/c mice. Four were selected for further study, from the panel of hybridomas. The antigen specificities of these MAbs were characterized and localized by enzyme-linked immunoeletrotransfer blot (EITB) and immunoperoxidase technique. The target epitopes of these MAbs are 66 kDa protein (MAb 2D10), 66 and 27-26 kDa proteins (MAbs 5D10 and 4F5) and 27-26 kDa protein (MAb 2D9). MAb 2D9 reacted to the antigenic components of the luminal content and epithelial cell lining the cecum, whereas MAb 2D10 reacted specifically to the antigens of the tegument and surface membrane. It was found that all MAbs cross-reacted to various degrees with the antigens extracted from Schistosoma mansoni, S. mekongi, S. spindale and Paramphistomum spp. However, when MAbs were diluted to 1:100 or 1:400 significant reduction of their cross-reactivities was observed.

[1]  G. Wijffels,et al.  Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica decreases worm fecundity. , 1994, Experimental parasitology.

[2]  A. Espino,et al.  Sandwich enzyme-linked immunosorbent assay for detection of excretory secretory antigens in humans with fascioliasis , 1994, Journal of clinical microbiology.

[3]  P. Sobhon,et al.  Monoclonal antibodies against Schistosoma mekongi surface tegumental antigens. , 1993, The Southeast Asian journal of tropical medicine and public health.

[4]  A. Morilla,et al.  Fasciola hepatica: characterization of somatic and excretory-secretory antigens of adult flukes recognized by infected sheep. , 1993, Revista latinoamericana de microbiologia.

[5]  J. C. Millán,et al.  Detection of antibodies and circulating excretory-secretory antigens for assessing cure in patients with fascioliasis. , 1992, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[6]  G. Hillyer,et al.  The purification and characterization of a cysteine protease of Fasciola gigantica adult worms. , 1992, Veterinary parasitology.

[7]  T. Kusamran,et al.  Characterization of two monoclonal antibodies against teguments of adult and schistosomula of Schistosoma japonicum. , 1991, Asian Pacific Journal of Allergy and Immunology.

[8]  H. Minocha,et al.  Production and characterization of monoclonal antibodies against excretory-secretory products of Fasciola hepatica. , 1991, Veterinary parasitology.

[9]  R. Marcet,et al.  Detection of circulating excretory secretory antigens in human fascioliasis by sandwich enzyme-linked immunosorbent assay , 1990, Journal of clinical microbiology.

[10]  G. Hillyer,et al.  Detection of circulating parasite antigen in murine fascioliasis by two-site enzyme-linked immunosorbent assays. , 1989, The American journal of tropical medicine and hygiene.

[11]  G. Hillyer,et al.  Cross-resistance between Schistosoma mansoni and Fasciola hepatica in sheep. , 1988, Journal of Parasitology.

[12]  G. Hillyer,et al.  Isolation of potential serodiagnostic Fasciola hepatica antigens by electroelution from polyacrylamide gels. , 1986, The American journal of tropical medicine and hygiene.

[13]  M. Taylor,et al.  Studies on heterologous resistance between Schistosoma bovis and Fasciola gigantica in Sudanese cattle , 1986, Journal of Helminthology.

[14]  G. Hillyer,et al.  Identification of functional Fasciola hepatica antigens in experimental infections in rabbits. , 1986, The American journal of tropical medicine and hygiene.

[15]  G. Hillyer.  Induction of immunity in mice to Fasciola hepatica with a Fasciola/Schistosoma cross-reactive defined immunity antigen. , 1985, The American journal of tropical medicine and hygiene.

[16]  V. Viyanant,et al.  Enzyme-linked immunosorbent assay for detection of Opisthorchis viverrini infection. , 1985, The Southeast Asian journal of tropical medicine and public health.

[17]  G. Hillyer,et al.  The antigens of Paragonimus westermani, Schistosoma mansoni, and Fasciola hepatica adult worms. Evidence for the presence of cross-reactive antigens and for cross-protection to Schistosoma mansoni infection using antigens of Paragonimus westermani. , 1983, The American journal of tropical medicine and hygiene.

[18]  M. Howell,et al.  Characterization of excretory-secretory antigens of Fasciola hepatica , 1982, Parasitology.

[19]  M. Sewell,et al.  A study of the antigens produced by adult Fasciola hepatica maintained in vitro , 1980 .

[20]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.