Fluorescence immunohistochemical detection of hypoxic cells in spheroids and tumours.

Polyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.

[1]  K. Krohn,et al.  Further characterization of 4-bromomisonidazole as a potential detector of hypoxic cells. , 1985, Radiation research.

[2]  D. Hirst,et al.  Changes in misonidazole binding with hypoxic fraction in mouse tumors. , 1985, International journal of radiation oncology, biology, physics.

[3]  G. Miller,et al.  An immunochemical probe for 8,5'-cycloadenosine-5'-monophosphate and its deoxy analog in irradiated nucleic acids. , 1985, Radiation research.

[4]  D. R. McCalla,et al.  Mode of Action of Nitrofurazone , 1970, Journal of bacteriology.

[5]  C. J. Koch,et al.  A novel technique for measuring human tissue pO2 at the cellular level. , 1986, British Journal of Cancer.

[6]  C. Koch,et al.  The radiation response of hypoxic cells in EMT6 spheroids in suspension culture does model data from EMT6 tumors. , 1983, Radiation research.

[7]  G. Whitmore,et al.  The radiation reduction in misonidazole. , 1981, Radiation research.

[8]  J. D. Chapman,et al.  A marker for hypoxic cells in tumours with potential clinical applicability. , 1981, British Journal of Cancer.

[9]  C. Song,et al.  Tumor reoxygenation and postirradiation vascular changes. , 1978, Radiology.

[10]  GUY SAINTE-MARIE,et al.  A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCE , 1962 .

[11]  A. Rauth,et al.  Electrophilic intermediate in the reactions of a 2-(hydroxyamino)imidazole. A model for biological effects of reduced nitroimidazoles , 1985 .

[12]  P. Allen,et al.  Covalent binding of a fluorinated 2-nitroimidazole to EMT-6 tumors in Balb/C mice: detection by F-19 nuclear magnetic resonance at 2.35 T. , 1986, International journal of radiation oncology, biology, physics.

[13]  J. D. Chapman,et al.  Characteristics of the metabolism-induced binding of misonidazole to hypoxic mammalian cells. , 1983, Cancer research.

[14]  J. Rasey,et al.  Comparison of binding of [3H]misonidazole and [14C]misonidazole in multicell spheroids. , 1985, Radiation research.

[15]  B. Teicher,et al.  The hypoxic tumor cell: a target for selective cancer chemotherapy. , 1980, Biochemical pharmacology.

[16]  A. Franko.  Misonidazole and other hypoxia markers: metabolism and applications. , 1986, International journal of radiation oncology, biology, physics.

[17]  J. D. Chapman,et al.  Intracellular localization of radioactively labeled misonidazole in EMT-6-tumor cells in vitro. , 1982, International journal of radiation oncology, biology, physics.

[18]  T. C. Jenkins,et al.  Induction of DNA strand breaks by reduced nitroimidazoles. Implications for DNA base damage. , 1986, Biochemical pharmacology.

[19]  L. Wiebe,et al.  Electron-Affillic Compounds for Labelling Hypoxie Cells : The Synthesis and Characterization of 1-[2-(2-Iodophenoxy)-Ethyl]-2-Nitroimidazole , 1984, Nuklearmedizin.

[20]  B. R. Smith,et al.  Hypoxia-enhanced reduction and covalent binding of [2-3H]misonidazole in the perfused rat liver. , 1984, Biochemical pharmacology.

[21]  R. McClelland,et al.  2-Hydroxylaminoimidazoles--unstable intermediates in the reduction of 2-nitroimidazoles. , 1984, Biochemical pharmacology.

[22]  J. Raleigh,et al.  Reductive fragmentation of 2-nitroimidazoles: amines and aldehydes. , 1984, International journal of radiation oncology, biology, physics.

[23]  B. R. Smith,et al.  Metabolism and excretion of [3H]misonidazole by hypoxic rat liver. , 1984, International journal of radiation oncology, biology, physics.

[24]  C. Koch,et al.  Binding of misonidazole to EMt6 and V79 spheroids. , 1982, International journal of radiation oncology, biology, physics.

[25]  B. Garrecht,et al.  The labelling of EMT-6 tumours in BALB/C mice with 14C-misonidazole. , 1983, The British journal of radiology.

[26]  A. J. Varghese.  Glutathione conjugates of misonidazole. , 1983, Biochemical and biophysical research communications.

[27]  C. Koch,et al.  Binding of 3H-misonidazole to solid human tumors as a measure of tumor hypoxia. , 1986, International journal of radiation oncology, biology, physics.

[28]  J. D. Chapman,et al.  Synthesis and in vivo studies of the radiosensitizer 4-[82Br]bromomisonidazole. , 1983, International journal of nuclear medicine and biology.

[29]  A. Franko.  Hypoxic fraction and binding of misonidazole in EMT6/Ed multicellular tumor spheroids. , 1985, Radiation Research.

[30]  C. Koch,et al.  Binding of misonidazole to hypoxic cells in monolayer and spheroid culture: evidence that a side-chain label is bound as efficiently as a ring label. , 1985, British Journal of Cancer.