Tryptophans 286 and 288 in the C-terminal Region of Protein B23.1 are Important for Its Nucleolar Localization

Nucleolar protein B23 can shuttle between the nucleolus and cytoplasm. However, the mechanism involved in the protein moving and staying in the nucleolus is not fully understood. To identify the nucleolar localization signal sequence of protein B23, we examined the subnuclear location of B23.1 mutant proteins fused with green fluorescent protein in HeLa cells. The results suggested that the two C-terminal tryptophan residues (Trp-286 and Trp-288) of protein B23.1 were important in this phenomenon.

[1]  M. Olson,et al.  Mapping the Functional Domains of Nucleolar Protein B23* , 2000, The Journal of Biological Chemistry.

[2]  H. Nakajima,et al.  Molecular characterization of a novel nucleolar protein in starfish oocytes which is phosphorylated before and during oocyte maturation. , 2000, European journal of biochemistry.

[3]  T. Akiyama,et al.  Nucleolar protein B23.1 binds to retinoblastoma protein and synergistically stimulates DNA polymerase alpha activity. , 1999, Journal of biochemistry.

[4]  R. Sloboda,et al.  Molecular Characterization of p62, a Mitotic Apparatus Protein Required for Mitotic Progression* , 1997, The Journal of Biological Chemistry.

[5]  J. Herrera,et al.  The ribonuclease activity of nucleolar protein B23. , 1995, Nucleic acids research.

[6]  H. Umekawa,et al.  Expression and subcellular locations of two forms of nucleolar protein B23 in rat tissues and cells. , 1993, Cellular & molecular biology research.

[7]  J. Gall,et al.  Localization of the nucleolar protein NO38 in amphibian oocytes , 1992, The Journal of cell biology.

[8]  T. Nguyen,et al.  Sequence-specific interaction of Tat protein and Tat peptides with the transactivation-responsive sequence element of human immunodeficiency virus type 1 in vitro. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[9]  D M Crothers,et al.  Fragments of the HIV-1 Tat protein specifically bind TAR RNA. , 1990, Science.

[10]  M. Maki,et al.  Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization , 1990, Journal of virology.

[11]  M. Maki,et al.  Functional similarity of HIV-I rev and HTLV-I rex proteins: identification of a new nucleolar-targeting signal in rev protein. , 1989, Biochemical and biophysical research communications.

[12]  J. Chang,et al.  A single gene codes for two forms of rat nucleolar protein B23 mRNA. , 1989, The Journal of biological chemistry.

[13]  C. Lehner,et al.  Major nucleolar proteins shuttle between nucleus and cytoplasm , 1989, Cell.

[14]  J. Chang,et al.  cDNA and deduced primary structure of rat protein B23, a nucleolar protein containing highly conserved sequences. , 1988, The Journal of biological chemistry.

[15]  W. Franke,et al.  A constitutive nucleolar protein identified as a member of the nucleoplasmin family. , 1987, The EMBO journal.

[16]  L. Cox,et al.  Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals. , 1987, The EMBO journal.

[17]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.