In vitro characterization of Trichophyton rubrum and T. mentagrophytes biofilms

Dermatophytes are fungi responsible for a disease known as dermatophytosis. Biofilms are sessile microbial communities surrounded by extracellular polymeric substances (EPS) with increased resistance to antimicrobial agents and host defenses. This paper describes, for the first time, the characteristics of Trichophyton rubrum and T. mentagrophytes biofilms. Biofilm formation was analyzed by light microscopy, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) as well as by staining with crystal violet and safranin. Metabolic activity was determined using the XTT reduction assay. Both species were able to form mature biofilms in 72 h. T. rubrum biofilm produced more biomass and EPS and was denser than T. mentagrophytes biofilm. The SEM results demonstrated a coordinated network of hyphae in all directions, embedded within EPS in some areas. Research and characterization of biofilms formed by dermatophytes may contribute to the search of new drugs for the treatment of these mycoses and might inform future revisions with respect to the dose and duration of treatment of currently available antifungals.

[1]  D. Lin,et al.  Metalloprotease genes of Trichophyton mentagrophytes are important for pathogenicity. , 2013, Medical mycology.

[2]  Zhang Jingxin,et al.  Biofilm formation by Fusarium oxysporum f. sp. cucumerinum and susceptibility to environmental stress. , 2014, FEMS microbiology letters.

[3]  L. A. Soares,et al.  Anti dermatophytic therapy - Prospects for the discovery of new drugs from natural products , 2013, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[4]  A. Beauvais,et al.  Investigation of Aspergillus fumigatus biofilm formation by various “omics” approaches , 2013, Front. Microbio..

[5]  A. Fusco-Almeida,et al.  Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. , 2013, Journal of medical microbiology.

[6]  S. Desantis,et al.  Biofilm formation of Malassezia pachydermatis from dogs. , 2012, Veterinary microbiology.

[7]  K. Ajesh,et al.  Cryptococcus laurentii Biofilms: Structure, Development and Antifungal Drug Resistance , 2012, Mycopathologia.

[8]  M. A. Resende-Stoianoff,et al.  Prevalence of Dermatomycosis in a Brazilian Tertiary Care Hospital , 2012, Mycopathologia.

[9]  A. Fusco-Almeida,et al.  Adhesion of Histoplasma capsulatum to pneumocytes and biofilm formation on an abiotic surface , 2012, Biofouling.

[10]  B. Regenberg,et al.  Saccharomyces cerevisiae--a model to uncover molecular mechanisms for yeast biofilm biology. , 2012, FEMS immunology and medical microbiology.

[11]  M. Monod,et al.  Mechanisms of skin adherence and invasion by dermatophytes , 2012, Mycoses.

[12]  A. Mitchell,et al.  Fungal Biofilms , 2012, PLoS pathogens.

[13]  T. C. White,et al.  A Foot in the Door for Dermatophyte Research , 2012, PLoS pathogens.

[14]  David W Williams,et al.  Microbiology of the skin and the role of biofilms in infection , 2012, International wound journal.

[15]  J. Lopez-Ribot,et al.  Addition of DNase improves the in vitro activity of antifungal drugs against Candida albicans biofilms , 2012, Mycoses.

[16]  S. Mandal,et al.  Effects of lactoferricin B against keratitis-associated fungal biofilms , 2012, Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy.

[17]  J. Zenilman,et al.  New horizons for cutaneous microbiology: the role of biofilms in dermatological disease , 2011, The British journal of dermatology.

[18]  A. Colombo,et al.  Current Knowledge of Trichosporon spp. and Trichosporonosis , 2011, Clinical Microbiology Reviews.

[19]  R. H. Pires,et al.  Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals. , 2011, Memorias do Instituto Oswaldo Cruz.

[20]  R. H. Pires,et al.  Anticandidal Efficacy of Cinnamon Oil Against Planktonic and Biofilm Cultures of Candida parapsilosis and Candida orthopsilosis , 2011, Mycopathologia.

[21]  H. Silveira,et al.  Isolation of transcripts overexpressed in the human pathogen Trichophyton rubrum grown in lipid as carbon source. , 2011, Canadian journal of microbiology.

[22]  P. Krzyściak,et al.  Dermatophyte species in superficial mycoses in the Kraków district, Poland in the years 1972–2007 , 2010, Mycoses.

[23]  D. E. Gras,et al.  Transcriptional profiling reveals genes in the human pathogen Trichophyton rubrum that are expressed in response to pH signaling. , 2010, Microbial pathogenesis.

[24]  D. E. Gras,et al.  Transcriptional profiling reveals the expression of novel genes in response to various stimuli in the human dermatophyte Trichophyton rubrum , 2010, BMC Microbiology.

[25]  Lyriam L. R. Marques,et al.  Can filamentous fungi form biofilms? , 2009, Trends in microbiology.

[26]  J. Lopez-Ribot,et al.  Our Current Understanding of Fungal Biofilms , 2009, Critical reviews in microbiology.

[27]  F. Müller,et al.  Aspergillus fumigatus Forms Biofilms with Reduced Antifungal Drug Susceptibility on Bronchial Epithelial Cells , 2008, Antimicrobial Agents and Chemotherapy.

[28]  B. Losson,et al.  Pathogenesis of Dermatophytosis , 2008, Mycopathologia.

[29]  M. Monod.  Secreted Proteases from Dermatophytes , 2008, Mycopathologia.

[30]  Craig Williams,et al.  Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. , 2007, Journal of medical microbiology.

[31]  L. Bakos,et al.  Freqüência das dermatofitoses em exames micológicos em Hospital Geral de Porto Alegre, Brasil , 2007 .

[32]  F. Müller,et al.  In vitro effects of micafungin against Candida biofilms on polystyrene and central venous catheter sections. , 2006, International journal of antimicrobial agents.

[33]  L. Truelstrup Hansen,et al.  Strain and growth temperature influence Listeria spp. attachment to intact and cut cabbage. , 2006, International journal of food microbiology.

[34]  M. Ghannoum,et al.  Modification of Surface Properties of Biomaterials Influences the Ability of Candida albicans To Form Biofilms , 2005, Applied and Environmental Microbiology.

[35]  M. Ghannoum,et al.  Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism , 2003, Journal of Clinical Microbiology.

[36]  C. Burkhart,et al.  Dermatophytoma: Recalcitrance to treatment because of existence of fungal biofilm. , 2002, Journal of the American Academy of Dermatology.

[37]  M. Ghannoum,et al.  Comparison of Biofilms Formed by Candidaalbicans and Candidaparapsilosis on Bioprosthetic Surfaces , 2002, Infection and Immunity.

[38]  B. Wickes,et al.  Characteristics of biofilm formation by Candida albicans. , 2001, Revista iberoamericana de micologia.

[39]  A. A. Machado,et al.  [Dermatophytic pseudomycetoma: report of a case caused by Trichophyton tonsurans]. , 2001, Revista da Sociedade Brasileira de Medicina Tropical.

[40]  J. J. C. Sidrim,et al.  Dermatofitoses no meio urbano e a coexistência do homem com cães e gatos , 1997 .

[41]  C. Morris,et al.  Methods for observing microbial biofilms directly on leaf surfaces and recovering them for isolation of culturable microorganisms , 1997, Applied and environmental microbiology.

[42]  R. Summerbell,et al.  The dermatophytes , 1995, Clinical microbiology reviews.

[43]  R. Hay,et al.  Adherence of dermatophyte microconidia and arthroconidia to human keratinocytes in vitro. , 1987, The Journal of investigative dermatology.