Subtractive hybridization magnetic bead capture: A new technique for the recovery of full‐length ORFs from the metagenome

A new method for the recovery of full‐length open reading frames from metagenomic nucleic acid samples is reported. This technique, based on subtractive hybridization magnetic bead capture technology, has the potential to access multiple gene variants from a single amplification reaction.

[1]  Emilio Fernández,et al.  Restriction enzyme site-directed amplification PCR: a tool to identify regions flanking a marker DNA. , 2005, Analytical biochemistry.

[2]  R. Bally,et al.  Polyphenol oxidase in Azospirillum lipoferum isolated from rice rhizosphere: Evidence for laccase activity in non-motile strains of Azospirillum lipoferum , 1993 .

[3]  J. Handelsman Metagenomics: Application of Genomics to Uncultured Microorganisms , 2004, Microbiology and Molecular Biology Reviews.

[4]  J. Tiedje,et al.  DNA recovery from soils of diverse composition , 1996, Applied and environmental microbiology.

[5]  C. Jacobsen Microscale detection of specific bacterial DNA in soil with a magnetic capture-hybridization and PCR amplification assay , 1995, Applied and environmental microbiology.

[6]  Yaoguang Liu,et al.  Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. , 1995, Genomics.

[7]  Ron Shamir,et al.  Theory and applications , 2004 .

[8]  Myriam Harry,et al.  Extraction and purification of microbial DNA from soil and sediment samples , 2001 .

[9]  H. Ruijssenaars,et al.  A cloned Bacillus halodurans multicopper oxidase exhibiting alkaline laccase activity , 2004, Applied Microbiology and Biotechnology.

[10]  C. Rensing,et al.  CueO is a multi-copper oxidase that confers copper tolerance in Escherichia coli. , 2001, Biochemical and biophysical research communications.

[11]  William Stafford,et al.  Metagenomic gene discovery: past, present and future. , 2005, Trends in biotechnology.

[12]  K. Schleifer,et al.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation. , 1995, Microbiological reviews.

[13]  F. Solano,et al.  Molecular cloning and functional characterization of a unique multipotent polyphenol oxidase from Marinomonas mediterranea. , 2001, Biochimica et biophysica acta.

[14]  S. Henikoff,et al.  Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. , 1998, Nucleic acids research.

[15]  J. Clapp,et al.  PCR-SSCP comparison of 16S rDNA sequence diversity in soil DNA obtained using different isolation and purification methods. , 2001, FEMS microbiology ecology.

[16]  F. Ayala,et al.  Use of polymerase chain reaction to amplify segments outside boundaries of known sequences. , 1993, Methods in enzymology.

[17]  L. Lange,et al.  Culture independent PCR: an alternative enzyme discovery strategy. , 2005, Journal of microbiological methods.

[18]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[19]  E. Madsen,et al.  Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Sediment Samples , 1999, Applied and Environmental Microbiology.

[20]  D. Cullen,et al.  Quantitation of fungal mRNAs in complex substrates by reverse transcription PCR and its application to Phanerochaete chrysosporium-colonized soil , 1995, Applied and environmental microbiology.

[21]  Jonathan D. Trent,et al.  DNA from Uncultured Organisms as a Source of 2,5-Diketo-d-Gluconic Acid Reductases , 2001, Applied and Environmental Microbiology.

[22]  A specific and versatile genome walking technique. , 2006, Gene.