Characterization of Interaction between Salvianolic Acid B and Phages Using Capillary Electrophoresis

Binding activity between Salvianolic acid B and selected phages from affinity biopanning in phage display library were studied with capillary electrophoresis based on the Hummel-Dreyer methods.The binding constant was calculated with Scatchard analysis using PRISM software.The results indicate that the surface proteins expressed by the phage clone interact specifically with Salvianolic acid B,and it is feasible to determine the binding constant between the selected phage and Salvianolic acid B using CE.