DEVELOPMENT OF KOMBUCHA AND ITS FUNCTIONAL PROPERTY FROM AGRICULTURAL WASTE (FERMENTED TEA BROTH)

The aim of this project was to develop a functional product from fermented tea broth which is a by-product of fermented tea (Miang). The broth that was from the 15th day fermented tea was used. The broth was boiled with the additional of 10% sugar for brix adjustment to make original and pineapple kombucha. Original fermented broth kombucha were produced form mixing with water and fermented broth (1:1.5) and add (1:1) pineapple for pineapple favor. After that, they were mixed with scoby and 3% of previous kombucha which produced from fermentation of Komagataeibacter saccharivorans, Zygosaccharomyces bailii, Dekkera bruxellensis. The analysis of antioxidant activity, total phenolic content and flavonoids content were performed at interval of 0, 1, 3, 5, 7, 9 and 11 day. The result showed that total phenolic of both original and pineapple kombucha were reduced. Total phenolic contents of original kombucha was in ranged of 32.1 42.05 μmol GAE/ 100 mL. Total phenolic contents of pineapple favour was in ranged of 23.1 ± 0.67 29.57 ± 1.19 μmol GAE/ 100 mL. While antioxidant (FRAP & ORAC) slightly increased in both batched. Antioxidant activity on FRAP of original kombucha was 311,527.8 ± 38,978.9 μmol TE / 100 mL. Antioxidant activity on ORAC of original kombucha and pineapple favor were highest at day 7 (620,442.1 ± 79,695.4 and 506,842 ± 212,017.1 μmol TE / 100 mL, respectively). Total flavonoids were decreased from 0th day fermented tea kombucha as it formed dimer and complex structure (10.15 – 6.92 μmol CE / 100 mL). Therefore, per serving size the developed kombuchas contained high amount of both phenolics and flavonoids. Fermented broth can be developed into Kombucha that containing functional property compounds.

[1]  Y. Feroniasanti,et al.  Effect of Fermentation to Total Titrable Acids, Flavonoid and Antioxidant Activity of Butterfly Pea Kombucha , 2019, Journal of Physics: Conference Series.

[2]  Sijie Tang,et al.  Use of kombucha consortium to transform soy whey into a novel functional beverage , 2019, Journal of Functional Foods.

[3]  C. Tamer,et al.  Kombucha Tea: A Promising Fermented Functional Beverage , 2019, Fermented Beverages.

[4]  D. Nielsen,et al.  Kombucha Beverage from Green, Black and Rooibos Teas: A Comparative Study Looking at Microbiology, Chemistry and Antioxidant Activity , 2018, Nutrients.

[5]  W. Srichamnong,et al.  Antioxidant of green tea and pickle tea product, miang, from northern Thailand , 2018 .

[6]  Amro B. Hassan,et al.  Estimation of Phenolic and Flavonoid Compounds and Antioxidant Activity of Spent Coffee and Black Tea (Processing) Waste for Potential Recovery and Reuse in Sudan , 2018, Recycling.

[7]  R. Piccoli,et al.  Probiotic potential of yeasts isolated from pineapple and their use in the elaboration of potentially functional fermented beverages. , 2018, Food research international.

[8]  F. Remize,et al.  Fruits and vegetables, as a source of nutritional compounds and phytochemicals: Changes in bioactive compounds during lactic fermentation. , 2017, Food research international.

[9]  E. Bourdon,et al.  Identification, stress tolerance, and antioxidant activity of lactic acid bacteria isolated from tropically grown fruits and leaves. , 2016, Canadian journal of microbiology.

[10]  A. Chatzinotas,et al.  Kombucha tea fermentation: Microbial and biochemical dynamics. , 2016, International journal of food microbiology.

[11]  N. Shah,et al.  Lactic acid bacterial fermentation modified phenolic composition in tea extracts and enhanced their antioxidant activity and cellular uptake of phenolic compounds following in vitro digestion , 2016 .

[12]  M. Hamdi,et al.  Development of a beverage from red grape juice fermented with the Kombucha consortium , 2016, Annals of Microbiology.

[13]  C. Chen,et al.  Effects of blending wheatgrass juice on enhancing phenolic compounds and antioxidant activities of traditional kombucha beverage , 2015, Journal of food and drug analysis.

[14]  M. Ayadi,et al.  Total Phenolic, Total Flavonoid, Tannin Content, and Antioxidant Capacity of Halimium halimifolium (Cistaceae) - , 2015 .

[15]  Jasmina Vitas,et al.  A Review on Kombucha Tea-Microbiology, Composition, Fermentation, Beneficial Effects, Toxicity, and Tea Fungus. , 2014, Comprehensive reviews in food science and food safety.

[16]  R. P. Ross,et al.  Sequence-based analysis of the bacterial and fungal compositions of multiple kombucha (tea fungus) samples. , 2014, Food microbiology.

[17]  J. Dwyer,et al.  Tea and flavonoids: where we are, where to go next. , 2013, The American journal of clinical nutrition.

[18]  Paul J. Moughan,et al.  Variation in antioxidant potential and total polyphenol content of fresh and fully-fermented Sri Lankan tea , 2011 .

[19]  N. Caffin,et al.  Phenolic compounds in tea from Australian supermarkets , 2006 .

[20]  N. Subramanian,et al.  Role of polyphenol oxidase and peroxidase in the generation of black tea theaflavins. , 1999, Journal of agricultural and food chemistry.