2D luminescence imaging of physiological wound oxygenation

Abstract:  In cutaneous wound healing, the role of oxygen in vivo is poorly understood. We studied wound surface pO2 during physiological wound healing in humans. Split‐thickness skin graft donor sites (n = 12) served as standardized wound models. Wound surface pO2 was measured at 1, 6 and 14 days after split‐skin harvesting using two‐dimensional luminescence lifetime imaging (2D‐LLI) of palladium(II)‐meso‐tetraphenyl‐tetrabenzoporphyrin (Pd‐TPTBP) in polystyrene‐co‐acrylonitrile (PSAN) particles on transparent foils. In another experiment, we removed the stratum corneum (SC) on the volar forearm (n = 10) by tape strippings to study the impact of the SC on the epidermal oxygen barrier. Split‐skin donor site pO2 significantly decreased during the time course of physiological healing. Regional differences in pO2 within donor site wounds were visualized for the first time in literature. No difference was found in pO2 before and after SC removal, showing that the SC is not a major constituent of the epidermal oxygen barrier.

[1]  P. Bastien,et al.  In vivo epidermal thickness measurement: ultrasound vs. confocal imaging , 2004, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[2]  T. Mayadas,et al.  Delayed wound healing in Mac‐1–deficient mice is associated with normal monocyte recruitment , 2007, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[3]  Timothy A. Dall,et al.  The burden of skin diseases: 2004 a joint project of the American Academy of Dermatology Association and the Society for Investigative Dermatology. , 2006, Journal of the American Academy of Dermatology.

[4]  A. Vahlquist,et al.  In vivo studies concerning a pH gradient in human stratum corneum and upper epidermis. , 1994, Acta dermato-venereologica.

[5]  A. Storrow,et al.  The Effect of Hyperbaric Oxygen Therapy on a Burn Wound Model in Human Volunteers , 1997, Plastic and reconstructive surgery.

[6]  I. Klimant,et al.  Photophysical properties of the new phosphorescent platinum(II) and palladium(II) complexes of benzoporphyrins and chlorins , 2009 .

[7]  G. Winter,et al.  Formation of the Scab and the Rate of Epithelization of Superficial Wounds in the Skin of the Young Domestic Pig , 1962, Nature.

[8]  Christian Krause,et al.  Composite Luminescent Material for Dual Sensing of Oxygen and Temperature , 2006 .

[9]  M. Landthaler,et al.  Wound healing in the 21st century. , 2010, Journal of the American Academy of Dermatology.

[10]  I Klimant,et al.  Red light-excitable oxygen sensing materials based on platinum(II) and palladium(II) benzoporphyrins. , 2008, Analytical chemistry.

[11]  N. Williams.  Birth defects , 2008, Current Biology.

[12]  W. Eaglstein,et al.  The effect of occlusive dressings on collagen synthesis and re-epithelialization in superficial wounds. , 1983, The Journal of surgical research.

[13]  E. Jung,et al.  Transcutaneous pO2 measurement during tourniquet-induced venous occlusion using dynamic phosphorescence imaging. , 2008, Clinical hemorheology and microcirculation.

[14]  Christoph Abels,et al.  Transcutaneous pO2 imaging during tourniquet‐induced forearm ischemia using planar optical oxygen sensors , 2008, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[15]  N. Aykin-Burns,et al.  Oxygen tension changes the rate of migration of human skin keratinocytes in an age‐related manner , 2011, Experimental dermatology.

[16]  A. Fichter,et al.  The Ideal Split-Thickness Skin Graft Donor Site Dressing: Rediscovery of Polyurethane Film , 2009, Annals of plastic surgery.

[17]  Christoph Abels,et al.  In Vivo Phosphorescence Imaging of pO2 Using Planar Oxygen Sensors , 2005, Microcirculation.

[18]  Michael Landthaler,et al.  The impact of the pH value on skin integrity and cutaneous wound healing , 2010, Journal of the European Academy of Dermatology and Venereology : JEADV.

[19]  H. Heise,et al.  Reflectance spectroscopy can quantify cutaneous haemoglobin oxygenation by oxygen uptake from the atmosphere after epidermal barrier disruption , 2003, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[20]  R WOLF,et al.  Continuous recording of blood oxygen tensions by polarography. , 1953, Journal of applied physiology.

[21]  M. Landthaler,et al.  Management of Split-Thickness Donor Sites With Synthetic Wound Dressings: Results of a Comparative Clinical Study , 2010, Annals of plastic surgery.

[22]  D. Lübbers,et al.  The cutaneous uptake of atmospheric oxygen contributes significantly to the oxygen supply of human dermis and epidermis , 2002, The Journal of physiology.

[23]  P. Zalesky,et al.  Topical oxygen emulsion: a novel wound therapy. , 2007, Archives of dermatology.

[24]  H. Wulf,et al.  Epidermal thickness at different body sites: relationship to age, gender, pigmentation, blood content, skin type and smoking habits. , 2003, Acta dermato-venereologica.

[25]  P. Altmeyer,et al.  Sauerstoffversorgung der Haut , 2004, Der Hautarzt.

[26]  D W Lübbers,et al.  The transepidermal oxygen flux from the environment is in balance with the capillary oxygen supply. , 2000, The Journal of investigative dermatology.

[27]  Otto S. Wolfbeis,et al.  Materials for fluorescence-based optical chemical sensors , 2005 .

[28]  A. Singer,et al.  Cutaneous wound healing. , 1999, The New England journal of medicine.

[29]  M. Landthaler,et al.  Oxygen in acute and chronic wound healing , 2010, The British journal of dermatology.

[30]  Motoji Takahashi,et al.  In vivo estimation of stratum corneum thickness from water concentration profiles obtained with Raman spectroscopy. , 2007, Acta dermato-venereologica.

[31]  Avner Friedman,et al.  Wound angiogenesis as a function of tissue oxygen tension: A mathematical model , 2008, Proceedings of the National Academy of Sciences.

[32]  P. Sejrsen,et al.  Oxygen tension and consumption measured by a tc‐Po2 electrode on heated skin before and after epidermal stripping , 1987, Acta anaesthesiologica Scandinavica.