Processing body autoantibodies reconsidered.

Processing bodies (P-bodies) are cellular structures that have critical roles in mRNA degradation and post-transcriptional gene silencing. Some patients with autoimmune disease have high titer antibodies directed against P-bodies, and certain sera have been used as markers for the GW182 component of these structures. This study shows that available reference sera are unreliable markers for GW182 because the sera contain antibodies directed against Ge-1, a second P-body autoantigen.

[1]  J. Lykke-Andersen,et al.  Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. , 2005, Molecular cell.

[2]  K. Bloch,et al.  Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body. , 2005, RNA.

[3]  E. Chan,et al.  Disruption of GW bodies impairs mammalian RNA interference , 2005, Nature Cell Biology.

[4]  J. Yates,et al.  A role for the P-body component GW182 in microRNA function , 2005, Nature Cell Biology.

[5]  Gregory J. Hannon,et al.  MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies , 2005, Nature Cell Biology.

[6]  H. Blau,et al.  Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies , 2005, Nature Cell Biology.

[7]  K. Lindor,et al.  The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis. , 2005, The Journal of rheumatology.

[8]  E. Chan,et al.  GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation , 2004, Journal of Cell Science.

[9]  B. Séraphin,et al.  Cytoplasmic foci are sites of mRNA decay in human cells , 2004, The Journal of cell biology.

[10]  B. Séraphin,et al.  The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies. , 2003, RNA.

[11]  R. Parker,et al.  Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies , 2003 .

[12]  S. Tenenbaum,et al.  A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles. , 2002, Molecular biology of the cell.

[13]  T. Quertermous,et al.  The immunoreactive region in a novel autoantigen contains a nuclear localization sequence. , 1994, Clinical immunology and immunopathology.