Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 104 rad/RIU, leading to a Limit of Detection below 5 × 10−7 RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.

[1]  T. Noguer,et al.  Development of an impedimetric aptasensor for the determination of aflatoxin M1 in milk. , 2016, Talanta.

[2]  L. Pavesi,et al.  Sensitivity and Limit of detection of biosensors based on ring resonators , 2015, 2015 XVIII AISEM Annual Conference.

[3]  Lech Wosinski,et al.  High-sensitivity liquid refractive-index sensor based on a Mach-Zehnder interferometer with a double-slot hybrid plasmonic waveguide. , 2015, Optics express.

[4]  B. Bilgicer,et al.  Oriented Immobilization of Fab Fragments by Site-Specific Biotinylation at the Conserved Nucleotide Binding Site for Enhanced Antigen Detection. , 2015, Langmuir : the ACS journal of surfaces and colloids.

[5]  Anh H. Nguyen,et al.  Fabrication of plasmon length-based surface enhanced Raman scattering for multiplex detection on microfluidic device. , 2015, Biosensors & bioelectronics.

[6]  Michael Thompson,et al.  Immobilization of Fab' fragments onto substrate surfaces: A survey of methods and applications. , 2015, Biosensors & bioelectronics.

[7]  Lorenzo Pavesi,et al.  Design and Optimization of SiON Ring Resonator-Based Biosensors for Aflatoxin M1 Detection , 2015, Sensors.

[8]  Claudio Piemonte,et al.  SPAD aptasensor for the detection of circulating protein biomarkers. , 2015, Biosensors & bioelectronics.

[9]  Dao-feng Liu,et al.  A modified lateral flow immunoassay for the detection of trace aflatoxin M1 based on immunomagnetic nanobeads with different antibody concentrations , 2015 .

[10]  A. Leinse,et al.  TriPleX: a versatile dielectric photonic platform , 2015 .

[11]  Huan Yang,et al.  A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array , 2014, International journal of nanomedicine.

[12]  Guo-Qiang Lo,et al.  Highly sensitive Mach–Zehnder interferometer biosensor based on silicon nitride slot waveguide , 2013 .

[13]  S. Hearty,et al.  A highly stable, sensitive, regenerable and rapid immunoassay for detecting aflatoxin B1 in corn incorporating covalent AFB1 immobilization and a recombinant Fab antibody. , 2013, Talanta.

[14]  Laura M. Lechuga,et al.  Integrated optical devices for lab‐on‐a‐chip biosensing applications , 2012 .

[15]  R. Heideman,et al.  TriPleX-Based Integrated Optical Ring Resonators for Lab-on-a-Chip and Environmental Detection , 2012, IEEE Journal of Selected Topics in Quantum Electronics.

[16]  A. Denizli,et al.  Fab fragments imprinted SPR biosensor for real-time human immunoglobulin G detection. , 2011, Biosensors & bioelectronics.

[17]  C. Limsakul,et al.  Detection of staphylococcal enterotoxin A (SEA) at picogram level by a capacitive immunosensor , 2011, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[18]  Keitaro Yoshimoto,et al.  Direct observation of adsorption-induced inactivation of antibody fragments surrounded by mixed-PEG layer on a gold surface. , 2010, Journal of the American Chemical Society.

[19]  Muzammil Iqbal,et al.  Label-Free Biosensor Arrays Based on Silicon Ring Resonators and High-Speed Optical Scanning Instrumentation , 2010, IEEE Journal of Selected Topics in Quantum Electronics.

[20]  J. Homola.  Surface plasmon resonance sensors for detection of chemical and biological species. , 2008, Chemical reviews.

[21]  L. Lechuga,et al.  An integrated optical interferometric nanodevice based on silicon technology for biosensor applications , 2003 .

[22]  Wolfgang Ehrfeld,et al.  A label-free affinity sensor with compensation of unspecific protein interaction by a highly sensitive integrated optical Mach–Zehnder interferometer on silicon , 1997 .