Analysis of images of cyanobacterial S-layers

Methods for analysis, especially 2D reconstruction, of cyanobacterial S-layers were improved. The locally normalized correlation was used in the course of correlation averaging. For elimination of image distortion, the function of the distortion was approximated step by step by the linear fractional function or by the Lagrange interpolation polynomials. The modification of the methods is illustrated on figures of S-layers of Synechocystis aquatilis Kovacik 1990/8 and Microcystis aeruginosa Hindak 1971/1 (negatively stained), and Microcystis cf. wesenbergii Bitov 1994 (metal shadow).