Cloning and characterization of a vasopressin V2 receptor and possible link to nephrogenic diabetes insipidus

THE antidiuretic effect of arginine vasopressin (AVP) is mediated by renal-type (V2) receptors linked to adenylyl cyclase1. We report here the cloning of the rat kidney V2 AVP receptor complementary DNA that encodes a 370-amino-acid protein with a transmembrane topography characteristic of G protein-coupled receptors, and with similarity to the Via (hepatic) AVP receptor2 in its seven membrane-spanning domains. Expression of the cloned cDNA in mammalian cells showed specific ligand binding and activity characteristic of the native V2 AVP receptor. The receptor messenger RNA is detected only in the kidney. The human V2 receptor gene has been localized to the long arm of the X chromosome close to the locus for nephrogenic diabetes insipidus, an X-linked recessive disorder characterized by renal resistance to the antidiuretic action of AVP3–6

[1]  E. Tribollet,et al.  Neurohypophyseal hormone receptor systems in brain and periphery. , 1987, Progress in brain research.

[2]  D. Dorsa,et al.  Characterization and localization of 3H-arginine8-vasopressin binding to rat kidney and brain tissue , 1983, Peptides.

[3]  M. Kozak.  Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. , 1984, Nucleic acids research.

[4]  H. Okayama,et al.  High-efficiency transformation of mammalian cells by plasmid DNA. , 1987, Molecular and cellular biology.

[5]  F. Antoni.  Characterization of high affinity binding sites for vasopressin in bovine adrenal medulla , 1984, Neuropeptides.

[6]  W. Young,et al.  Tyrosine hydroxylase mRNA is increased by hyperosmotic stimuli in the paraventricular and supraoptic nuclei. , 1987, Neuroendocrinology.

[7]  A. Olsen,et al.  Number and size of human X chromosome fragments transferred to mouse cells by chromosome-mediated gene transfer , 1981, Molecular and cellular biology.

[8]  M. Brownstein,et al.  Molecular cloning and expression of a rat Via arginine vasopressin receptor , 1992, Nature.

[9]  T. Bonner,et al.  Mesencephalic dopamine neurons regulate the expression of neuropeptide mRNAs in the rat forebrain. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[10]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[11]  D. Hirsch,et al.  Epinephrine and dDAVP administration in patients with congenital nephrogenic diabetes insipidus. Evidence for a pre-cyclic AMP V2 receptor defective mechanism. , 1989, Kidney international.

[12]  V. Mooser,et al.  Localization of vasopressin binding sites in rat tissues using specific V1 and V2 selective ligands. , 1990, Endocrinology.

[13]  J. Crawford,et al.  Nephrogenic diabetes insipidus in North America. The Hopewell hypothesis. , 1969, The New England journal of medicine.

[14]  M. Manning,et al.  Potent and selective antagonists of the antidiuretic responses to arginine-vasopressin based on modifications of [1-(beta-mercapto-beta,beta-pentamethylenepropionic acid),2-D-isoleucine,4- valine]arginine-vasopressin at position 4. , 1984, Journal of medicinal chemistry.

[15]  H. Lodish,et al.  Expression cloning of an adenylate cyclase-coupled calcitonin receptor. , 1991, Science.

[16]  M. Frohman,et al.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[17]  S. Nakanishi,et al.  Cloning and expression of a cDNA encoding an endothelin receptor , 1990, Nature.

[18]  P. Leder,et al.  Localization of human variable and constant region immunoglobulin heavy chain genes on subtelomeric band q32 of chromosome 14. , 1982, Nucleic acids research.

[19]  N. Knoers,et al.  Linkage of X-linked nephrogenic diabetes insipidus with DXS52, a polymorphic DNA marker. , 1988, Nephron.

[20]  S. Jard.  Vasopressin Isoreceptors in Mammals: Relation to Cyclic AMP-Dependent and Cyclic AMP-Independent Transduction Mechanisms , 1983 .

[21]  M. Manning,et al.  [1-beta-Mercapto-beta,beta-cyclopentamethylenepropionic acid),2-(O-methyl)tyrosine ]argine-vasopressin and [1-beta-mercapto-beta,beta-cyclopentamethylenepropionic acid)]argine-vasopressine, two highly potent antagonists of the vasopressor response to arginine-vasopressin. , 1980, Journal of medicinal chemistry.

[22]  M. Freeman,et al.  A G protein-linked receptor for parathyroid hormone and parathyroid hormone-related peptide. , 1991, Science.

[23]  C. Kirk,et al.  Rapid stimulation, by vasopressin and adrenaline, of inorganic phosphate incorporation into phosphatidyl inositol in isolated hepatocytes , 1977, FEBS letters.

[24]  F. Fahrenholz,et al.  Derivatives of somatic cell hybrids which carry the human gene locus for nephrogenic diabetes insipidus (NDI) express functional vasopressin renal V2-type receptors. , 1990, The Journal of biological chemistry.

[25]  S. Seino,et al.  Cloning and functional characterization of a family of human and mouse somatostatin receptors expressed in brain, gastrointestinal tract, and kidney. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[26]  L. Mahan,et al.  Method for identifying ligands that bind to cloned Gs- or Gi-coupled receptors , 1991, Molecular and Cellular Neuroscience.