Purification and characterization of diamine oxidase (histaminase) from rat small intestine.

Diamine oxidase (DAO) was purified to homogeneity from rat small intestine, and its biochemical and immunochemical properties were studied. DAO was suggested to be a dimer of a 92 kDa subunit, and its isoelectric point was found to be 6.0. Histamine, putrescine, N tau-methylhistamine, and cadaverine were good substrates, with Km values ranging from 9.4 to 16.0 microM. Spermine and spermidine were not substrates. Both an immunoprecipitation study and Ouchterlony's double diffusion test involving antiserum against the purified DAO showed that the immunological properties of the DAOs from rat small intestine, thymus, and placenta were identical. Among small intestinal DAOs from different species, this antibody reacted to the guinea pig enzyme as strongly as to the rat enzyme, but the reaction was much weaker to the mouse enzyme than to the rat enzyme. The DAOs from rabbit and dog small intestine, pig kidney, and human placenta showed no reactivity toward this antibody.

[1]  M. Lazdunski,et al.  Diamine oxidase is the amiloride-binding protein and is inhibited by amiloride analogues. , 1994, The Journal of biological chemistry.

[2]  M. Lazdunski,et al.  Molecular cloning and functional expression of different molecular forms of rat amiloride-binding proteins. , 1993, European journal of biochemistry.

[3]  M. A. Shah,et al.  The glycoprotein nature of pig kidney diamine oxidase. Role of disulphide groups and arginine residues in the concanavalin A-diamine oxidase interaction. , 1988, The Biochemical journal.

[4]  O. Suzuki,et al.  Purification and properties of diamine oxidase from human kidney , 1987 .

[5]  T. Splinter,et al.  Species-dependent differences of the biochemical properties of diamine oxidase. , 1986, The International journal of biochemistry.

[6]  W. Lorenz,et al.  Distribution and properties of human intestinal diamine oxidase and its relevance for the histamine catabolism. , 1983, Biochimica et biophysica acta.

[7]  T. Biegański.  Biochemical, physiological and pathophysiological aspects of intestinal diamine oxidase. , 1983, Acta physiologica Polonica.

[8]  G. Floris,et al.  Diamine oxidase from pig kidney: new purification method and amino acid composition. , 1982, Preparative biochemistry.

[9]  W. Gahl,et al.  Spermidine oxidase in human pregnancy serum. Probable identity with diamine oxidase. , 1982, The Biochemical journal.

[10]  P. Burke,et al.  Diamine oxidase as a plasma marker of rat intestinal mucosal injury and regeneration after administration of 1-beta-D-arabinofuranosylcytosine. , 1981, Cancer research.

[11]  C. W. Lin,et al.  Tumor and placental histaminase, I. affinity chromatography purification and characterization of the placental enzyme. , 1981, Oncodevelopmental biology and medicine : the journal of the International Society for Oncodevelopmental Biology and Medicine.

[12]  C. W. Lin,et al.  Tumor and placental histaminase, II A procedure for radioimmunoassay. , 1981, Oncodevelopmental biology and medicine : the journal of the International Society for Oncodevelopmental Biology and Medicine.

[13]  S. Baylin,et al.  Diamine oxidase (histaminase). A circulating marker for rat intestinal mucosal maturation and integrity. , 1980, The Journal of clinical investigation.

[14]  S. Margolis,et al.  Localization of histamine (diamine oxidase) in rat small intestinal mucosa: site of release by heparin. , 1977, Biochemical pharmacology.

[15]  S. Baylin.  Histaminase (diamine oxidase) activity in human tumors: an expression of a mature genome. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[16]  W. Bardsley,et al.  Human kidney diamine oxidase: inhibition studies. , 1976, Biochemical pharmacology.

[17]  P. Knowles,et al.  Human placental diamine oxidase. Improved purification and characterization of a copper- and manganese-containing amine oxidase with novel substrate specificity. , 1976, The Biochemical journal.

[18]  M. Beaven,et al.  Turnover and synthesis of diamine oxidase (DAO) in rat tissues. Studies with heparin and cycloheximide. , 1976, Biochemical pharmacology.

[19]  S. Margolis,et al.  Purification of histaminase (diamine oxidase) from human pregnancy plasma by affinity chromatography. , 1975, Biochimica et biophysica acta.

[20]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[21]  J. K. Smith.  The purification and properties of placental histaminase. , 1967, The Biochemical journal.

[22]  F. Buffoni.  Histaminase and related amine oxidases. , 1966, Pharmacological reviews.

[23]  O OUCHTERLONY,et al.  Diffusion-in-gel methods for immunological analysis. , 1958, Progress in allergy.

[24]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[25]  J. Janne,et al.  Oxidation of Polyamines by Diamine Oxidase from Human Seminal Plasma , 2022 .