A new sensing material for optical oxygen measurement, with the indicator embedded in an aqueous phase

A new type of oxygen-sensitive material is obtained by preparing an aqueous emulsion of a solution of an oxygen-sensitive fluorescent dye in a rigid polymer. The fluorescence of this emulsion is related to the oxygen partial pressure, but a Stern-Volmer plot is not linear over the whole pressure range. Aside from high sensitivity and specificity for oxygen, this new type of sensing material has favorable analytical wavelengths allowing the use of low-cost opto-electronic equipment. Since the indicator is embedded in an aqueous environment, the sensor should be capable of monitoring various kinds of reactions occurring in the aqueous phase, for instance enzymatic reactions which are accompanied by production or consumption of oxygen.

[1]  Otto S. Wolfbeis,et al.  Fibre-optic fluorescing sensor for ammonia , 1986 .

[2]  M E Cox,et al.  Detection of oxygen by fluorescence quenching. , 1985, Applied optics.

[3]  E. Bechara,et al.  Ruthenium(II) tris(bipyridyl) ion as a luminescent probe for oxygen uptake. , 1986, Analytical biochemistry.

[4]  N. Opitz,et al.  Opticl fluorescence sensors for continuous measurement of chemical concentrations in biological systems , 1983 .

[5]  Frank V. Bright,et al.  Fluorescence spectra and lifetimes of several fluorophores immobilized on nonionic resins for use in fiber-optic sensors , 1987 .

[6]  C. Bartolomei,et al.  Disopyramide-induced QT prolongation and ventricular tachyarrhythmias. , 1983, American heart journal.

[7]  H. Marsoner,et al.  A fluorescence-based sterilizable oxygen probe for use in bioreactors☆ , 1983 .

[8]  H. Kautsky,et al.  Energie‐Umwandlungen an Grenzflächen, VI. Mitteil.: Kohlensäure‐Assimilation (1.) , 1932 .

[9]  O. Wolfbeis,et al.  Fluorescence sensor for monitoring ionic strength and physiological pH values , 1986 .

[10]  C. R. Martin,et al.  Luminescence Titrations of Polyelectrolytes. , 1984 .

[11]  O. Wolfbeis,et al.  Fiber optical fluorosensor for determination of halothane and or oxygen , 1985 .

[12]  H. Kautsky,et al.  Nachweis geringster Sauerstoffmengen durch Phosphoreszenztilgung , 1935 .

[13]  O. Wolfbeis.  Fiber-optic probe for kinetic determination of enzyme activities. , 1986, Analytical chemistry.

[14]  Helmut Offenbacher,et al.  Fluorescence optical sensors for continuous determination of near-neutral pH values , 1986 .

[15]  Otto S. Wolfbeis,et al.  Fluorescence optical sensors in analytical chemistry , 1985 .

[16]  I. Bergman,et al.  Rapid-response Atmospheric Oxygen Monitor based on Fluorescence Quenching , 1968, Nature.

[17]  J. Peterson,et al.  Fiber-optic probe for in vivo measurement of oxygen partial pressure. , 1984, Analytical chemistry.