Preparation and application of surface-coated superparamagnetic nanobeads in the isolation of genomic DNA

A novel method was developed to prepare functionalized magnetic beads, in which superparamagnetic Fe3O4 core was synthesized with an injection-precipitation method and then was coated with functional groups using one-step suspension polymerization. In the coating and functionalizing process, a unique coupling reagent, bis-(2-hydroxyethyl methacrylate) phosphate, was introduced so that the monomers polymerized only on the surface of the nanocrystals without forming separate nuclei. The thickness of the coating layer and the size and density of the coated nanobeads were controlled by changing the quantity of the coated monomers. The nanobeads were characterized by transmission electron microscopy, light scattering spectrometry, Fourier transformation infrared spectroscopy, X-ray fluorescence spectroscopy and the magnetic hysteresis loop determination method. The carboxyl-modified magnetic nanobeads were employed to simplify the isolation of genomic DNA from human whole blood.

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