DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance

Staphylococcus aureus gyrA and gyrB genes, which encode the DNA gyrase A and B proteins, have been isolated and found to map contiguously. DNA sequence analysis revealed close homology between the S. aureus gyrase subunits and their counterparts in Bacillus subtilis and Escherichia coli, including several conserved amino acid residues whose substitution in E. coli confers resistance to 4-quinolones. These results are discussed in regard to quinolone resistance mechanisms in S. aureus.

[1]  T. Andoh,et al.  [Inhibitors of DNA topoisomerases]. , 1990, Gan to kagaku ryoho. Cancer & chemotherapy.

[2]  K. Ubukata,et al.  Cloning and expression of the norA gene for fluoroquinolone resistance in Staphylococcus aureus , 1989, Antimicrobial Agents and Chemotherapy.

[3]  A. Wyke,et al.  Cloning and characterization of a DNA gyrase A gene from Escherichia coli that confers clinical resistance to 4-quinolones , 1989, Antimicrobial Agents and Chemotherapy.

[4]  I. Shalit,et al.  Widespread quinolone resistance among methicillin-resistant Staphylococcus aureus isolates in a general hospital , 1989, Antimicrobial Agents and Chemotherapy.

[5]  S. Schaefler.  Methicillin-resistant strains of Staphylococcus aureus resistant to quinolones , 1989, Journal of clinical microbiology.

[6]  P. Mackowiak,et al.  Ciprofloxacin for methicillin-resistant Staphylococcus aureus infections , 1989, Antimicrobial Agents and Chemotherapy.

[7]  J. Smith,et al.  CIPROFLOXACIN RESISTANCE IN EPIDEMIC METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS , 1988, The Lancet.

[8]  T. Nishino,et al.  DNA gyrase of Staphylococcus aureus and inhibitory effect of quinolones on its activity , 1988, Antimicrobial Agents and Chemotherapy.

[9]  J. Wang,et al.  Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase. , 1987, Journal of molecular biology.

[10]  J. Wang,et al.  Mapping the active site tyrosine of Escherichia coli DNA gyrase. , 1987, The Journal of biological chemistry.

[11]  R. Skurray,et al.  Antimicrobial resistance of Staphylococcus aureus: genetic basis , 1987, Microbiological reviews.

[12]  E. Appella,et al.  DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. , 1987, Nucleic acids research.

[13]  S. Moriya,et al.  Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli. , 1985, The EMBO journal.

[14]  D. Hooper,et al.  The fluoroquinolones: structures, mechanisms of action and resistance, and spectra of activity in vitro , 1985, Antimicrobial Agents and Chemotherapy.

[15]  R. Eng,et al.  Activity of ciprofloxacin against methicillin-resistant Staphylococcus aureus , 1985, Antimicrobial Agents and Chemotherapy.

[16]  S. Moriya,et al.  Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region. , 1985, Nucleic acids research.

[17]  Mary F. LAMPEt,et al.  Genetic and physical organization of the cloned gyrA and gyrB genes of Bacillus subtilis , 1985, Journal of bacteriology.

[18]  P. V. von Hippel,et al.  Rho-dependent termination of transcription. II. Kinetics of mRNA elongation during transcription from the bacteriophage lambda PR promoter. , 1983, The Journal of biological chemistry.

[19]  M. Uhlén,et al.  Gene for staphylococcal protein A. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[20]  M. Gellert,et al.  DNA gyrase: subunit structure and ATPase activity of the purified enzyme. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[21]  P. Brown,et al.  Energy coupling in DNA gyrase and the mechanism of action of novobiocin. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[22]  N. Cozzarelli,et al.  Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[23]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[24]  M. Gellert,et al.  Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[25]  M. Gellert,et al.  DNA gyrase: an enzyme that introduces superhelical turns into DNA. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[26]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[27]  D. Hogness,et al.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[28]  D. Crothers,et al.  Improved estimation of secondary structure in ribonucleic acids. , 1973, Nature: New biology.

[29]  T. H. Wood,et al.  Escherichia coli K-12 Mutants Resistant to Nalidixic Acid: Genetic Mapping and Dominance Studies , 1969, Journal of bacteriology.

[30]  W. Goss,et al.  Mechanism of Action of Nalidixic Acid on Escherichia coli III. Conditions Required for Lethality , 1966, Journal of bacteriology.

[31]  W. Goss,et al.  Mechanism of Action of Nalidixic Acid on Escherichia coli II. Inhibition of Deoxyribonucleic Acid Synthesis , 1965, Journal of bacteriology.

[32]  W. Gilbert,et al.  Sequencing end-labeled DNA with base-specific chemical cleavages. , 1980, Methods in enzymology.