Stacked centrifugal microfluidic device with three-dimensional microchannel networks and multifunctional capillary bundle structures for immunoassay

Abstract A stacked-type centrifugal microfluidic device with three-dimensional (3D) microchannel networks and multifunctional capillary bundle structures is developed, and an immunoassay for the detection of immunoglobulin G (IgG) is demonstrated. The device consists of multiple layers of disk-like chips with conventional planar microchannels and vertical capillary bundle structures that are constructed on disks of poly(dimethylsiloxane) and poly(methyl methacrylate). The integration of multiple reactors up to 10 reactors on a disk with 8 cm in diameter is realized by reducing the area of reservoir but increasing the thickness. Moreover the thick optical detection reservoir is also realized for absorption spectroscopy in the 3D centrifugal microfluidics. 3D liquid valving on the vertical capillary bundle structures and liquid transportation through these structures are successfully demonstrated. The results show the successful control of the multistep liquid injection without problems such as blockage of capillary bundle by bubbles that expected to be caused due to 3D structure. The results of the immunoassay show clear response to analyte concentration suggesting successful unit operations in developed 3D centrifugal microfluidic device for immunoassay. Finally, the advantages and impact of the 3D design for the development of high-performance centrifugal microfluidic systems are summarized.

[1]  Masatoshi Nomura,et al.  Development of multi-layered microreactor with methanol reformer for small PEMFC , 2005 .

[2]  D. J. Harrison,et al.  Microfabrication of a Planar Absorbance and Fluorescence Cell for Integrated Capillary Electrophoresis Devices , 1996 .

[3]  Shengnian Wang,et al.  Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay. , 2004, Analytical chemistry.

[4]  Yoon‐Kyoung Cho,et al.  Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices. , 2007, Lab on a chip.

[5]  Robin H. Liu,et al.  Passive mixing in a three-dimensional serpentine microchannel , 2000, Journal of Microelectromechanical Systems.

[6]  R. Zengerle,et al.  Fully integrated whole blood testing by real-time absorption measurement on a centrifugal platform. , 2006, Lab on a chip.

[7]  A Ando,et al.  Isochronous storage ring of the New SUBARU project. , 1998, Journal of synchrotron radiation.

[8]  G. Whitesides,et al.  Flexible Methods for Microfluidics , 2001 .

[9]  N. B. Trung,et al.  Multi-chamber PCR chip with simple liquid introduction utilizing the gas permeability of polydimethylsiloxane , 2010 .

[10]  Enzyme-linked immunosorbent assay for nonylphenol using antibody-bound microfluid filters in vertical fluidic operation. , 2007, Journal of bioscience and bioengineering.

[11]  T Kitamori,et al.  Determination of carcinoembryonic antigen in human sera by integrated bead-bed immunoassay in a microchip for cancer diagnosis. , 2001, Analytical chemistry.

[12]  Yoon‐Kyoung Cho,et al.  A fully automated immunoassay from whole blood on a disc. , 2009, Lab on a chip.

[13]  Marc J Madou,et al.  The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform. , 2010, Lab on a chip.

[14]  Hai-Qing Gong,et al.  Micro air bubble formation and its control during polymerase chain reaction (PCR) in polydimethylsiloxane (PDMS) microreactors , 2007, Journal of Micromechanics and Microengineering.

[15]  George M Whitesides,et al.  Prototyping of microfluidic devices in poly(dimethylsiloxane) using solid-object printing. , 2002, Analytical chemistry.

[16]  M. Tokeshi,et al.  Glass microchip with three-dimensional microchannel network for 2 x 2 parallel synthesis. , 2002, Lab on a chip.

[17]  Jitae Kim,et al.  Cell lysis on a microfluidic CD (compact disc). , 2004, Lab on a chip.

[18]  Marc Madou,et al.  Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform. , 2011, Lab on a chip.

[19]  D. Leckband,et al.  Development and characterization of an ELISA assay in PDMS microfluidic channels , 2001 .

[20]  Yuichi Utsumi,et al.  Proposal of a new microreactor for vertical chemical operation , 2006 .

[21]  R Zengerle,et al.  Batch-mode mixing on centrifugal microfluidic platforms. , 2005, Lab on a chip.

[22]  C. Shu,et al.  Chaotic micromixers using two-layer crossing channels to exhibit fast mixing at low Reynolds numbers. , 2005, Lab on a chip.

[23]  R. Zengerle,et al.  Visualization of flow patterning in high-speed centrifugal microfluidics , 2005 .

[24]  R P Mason,et al.  The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates. , 1982, The Journal of biological chemistry.

[25]  R. Zengerle,et al.  Centrifugal extraction of plasma from whole blood on a rotating disk. , 2006, Lab on a chip.

[26]  Tadashi Hattori,et al.  Large-Area X-ray Lithography System for LIGA Process Operating in Wide Energy Range of Synchrotron Radiation , 2005 .

[27]  T Kitamori,et al.  Integration of an immunosorbent assay system: analysis of secretory human immunoglobulin A on polystyrene beads in a microchip. , 2000, Analytical chemistry.

[28]  Shigeru Nakagawa,et al.  Micropump and sample-injector for integrated chemical analyzing systems , 1990 .

[29]  Yoon-Kyoung Cho,et al.  One-Step Pathogen Specific DNA Extraction from Whole Blood on a Centrifugal Microfluidic Device , 2007, TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference.

[30]  Yuichi Utsumi,et al.  Novel Characteristics of Multifunctional Fluid Filters Fabricated by High-Energy Synchrotron Radiation Lithography , 2006 .

[31]  Sylvia Daunert,et al.  Design and Fabrication of CD-Like Microfluidic Platforms for Diagnostics: Polymer-Based Microfabrication , 2001 .

[32]  Y. Utsumi,et al.  Application of vertical microreactor stack with polystylene microbeads to immunoassay , 2008 .

[33]  D. J. Harrison,et al.  A multireflection cell for enhanced absorbance detection in microchip‐based capillary electrophoresis devices , 2000, Electrophoresis.

[34]  Chun-Ping Jen,et al.  Design and simulation of the micromixer with chaotic advection in twisted microchannels. , 2003, Lab on a chip.

[35]  G M Whitesides,et al.  Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. , 2000, Analytical chemistry.

[36]  Joe T. Lin,et al.  Microfabricated Centrifugal Microfluidic Systems: Characterization and Multiple Enzymatic Assays , 1999 .

[37]  E. Tamiya,et al.  Label-free immunosensor for prostate-specific antigen based on single-walled carbon nanotube array-modified microelectrodes. , 2007, Biosensors & bioelectronics.

[38]  P. Andersson,et al.  Simultaneous multiple immunoassays in a compact disc-shaped microfluidic device based on centrifugal force. , 2005, Clinical chemistry.

[39]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[40]  Yuichi Utsumi,et al.  Large area and wide dimension range x-ray lithography for lithographite, galvanoformung, and abformung process using energy variable synchrotron radiation , 2005 .

[41]  J. Rossier,et al.  Enzyme linked immunosorbent assay on a microchip with electrochemical detection. , 2001, Lab on a chip.

[42]  Marc Madou,et al.  Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions , 2001 .

[43]  Wanjun Wang,et al.  A rapid micro-mixer/reactor based on arrays of spatially impinging micro-jets , 2004 .

[44]  R. Zengerle,et al.  Frequency-dependent transversal flow control in centrifugal microfluidics. , 2005, Lab on a chip.