Multiplex PCR amplification of TH01, D9S304, and D3S1744 loci

AbstractA multiplex typing method of the tetrameric short tandem repeat (STR) loci TH01, D9S304, and D3S1744 was developed. The allelic ladder included alleles 6–11 (80–100 bp) and 9.3 (95 bp) for TH01, alleles 6–15 (125–161 bp) for D9S304, and alleles 13–22 (174–210 bp) for D3S1744. The observed heterozygosity of D9S304 was 0.851. The combined discrimination power of the three loci was 0.991.