Studies on the applying of embryo culture in breeding new hybrids by crossing seedless grape cultivars

This study was carried out to search the possibilities of propagating plants from ovules and embryos of seedless x seedless table grape crosses. Ovules of 3A/261x Bans and 15/A-61x Bans crosses cultured 42 and 52 days after artificial pollination. E20A and ER + WP medias were used in culturing of ovules and embryos. It had been observed that there was no difference between the periods of time in according to viable embryo number for two crosses in E20A media. In ER media, only 3/A-261x Bans ovules which had been taken 52 days after pollination gave higher viable embryo number than 42 days. The germination rate of viable embryos of 3/A-261x Baris combination was at least in the 42 th day + ER treatment. These ratios were 31.52% in ER media and 80.21% in E20A media for ovules of 15/A-61 x Bans that had been cultured in 52 th day. The treatment of 52 th day + E20A media gave the highest number (21.77%) of seedlings through ovules of 15/A-61x Bans combination. This number was lower in both two periods and two medias for 3/A-261x Barn ovules.