Regulation of atrial natriuretic hormone production by triiodothyronine in cultured rat atrial myocytes.

Since thyroid hormones have been reported to increase the synthesis of rat atrial natriuretic hormone and its mRNA accumulation, we further investigated the mechanism of stimulation of rANH synthesis by T3 using cultured rat atrial myocytes. T3 (10(-9)-10(-7) mol/l) increased cellular content and secretion into the medium of immunoreactive rANH in a dose-dependent manner. However, the ratio of secreted/cellular rANH was not altered by the addition of T3. Furthermore, in both cellular and secreted rANH, the larger molecular form (gamma-rANH) apparently predominated over the smaller one (alpha-rANH), and this profile was not influenced by T3 (10(-7) mol/l). Although T3 (10(-9)-10(-7) mol/l) also increased cellular rANH mRNA accumulation, the ratio of cellular rANH/rANH mRNA was not changed. Moreover, using actinomycin D, we found that T3 (10(-7) mol/l) did not affect the degradation of rANH mRNA. From these findings, we suggest that T3 has no specific effects on the translational and posttranslational processes and the release of rANH, but that T3 stimulates rANH synthesis in the pretranslational levels, probably in the transcriptional level of rANH gene.

[1]  Y. Mori,et al.  Iodothyronine 5'-deiodinase activity in cultured rat myocardial cells: characteristics and effects of triiodothyronine and angiotensin II. , 1991, Endocrinology.

[2]  D. Waxman,et al.  Pretranslational control by thyroid hormone of rat liver steroid 5 alpha-reductase and comparison to the thyroid dependence of two growth hormone-regulated CYP2C mRNAs. , 1990, The Journal of biological chemistry.

[3]  M. Lazar,et al.  Thyroid hormone receptors form distinct nuclear protein-dependent and independent complexes with a thyroid hormone response element. , 1990, Molecular endocrinology.

[4]  S. Stapleton,et al.  Triiodothyronine stimulates transcription of the fatty acid synthase gene in chick embryo hepatocytes in culture. Insulin and insulin-like growth factor amplify that effect. , 1990, The Journal of biological chemistry.

[5]  B. Desvergne,et al.  Identification of a thyroid hormone response element in the malic enzyme gene. , 1990, The Journal of biological chemistry.

[6]  B. Markham,et al.  An anti-sense c-erbA clone inhibits thyroid hormone-induced expression from the alpha-myosin heavy chain promoter. , 1990, The Journal of biological chemistry.

[7]  J. Lingrel,et al.  Thyroid and glucocorticoid hormones regulate the expression of multiple Na,K-ATPase genes in cultured neonatal rat cardiac myocytes. , 1990, The Journal of biological chemistry.

[8]  Y. Mori,et al.  Stimulation of rat atrial natriuretic peptide (rANP) synthesis by triiodothyronine and thyroxine (T4): T4 as a prohormone in synthesizing rANP. , 1990, Endocrinology.

[9]  D. Gardner,et al.  Gene for the rat atrial natriuretic peptide is regulated by glucocorticoids in vitro. , 1988, The Journal of clinical investigation.

[10]  H. Matsubara,et al.  Role of calcium and protein kinase C in ANP secretion by cultured rat cardiocytes. , 1988, The American journal of physiology.

[11]  J. Seidman,et al.  Modulation of atrial natriuretic factor by thyroid hormone: messenger ribonucleic acid and peptide levels in hypothyroid, euthyroid, and hyperthyroid rat atria and ventricles. , 1988, Endocrinology.

[12]  P. Ladenson,et al.  Plasma atriopeptin concentrations in hyperthyroidism, euthyroidism, and hypothyroidism: studies in man and rat. , 1987, The Journal of clinical endocrinology and metabolism.

[13]  H. Matsubara,et al.  Ventricular myocytes from neonatal rats are more responsive to dexamethasone than atrial myocytes in synthesis of atrial natriuretic peptide. , 1987, Biochemical and biophysical research communications.

[14]  H. Matsubara,et al.  Effects of steroid and thyroid hormones on synthesis of atrial natriuretic peptide by cultured atrial myocytes of rat. , 1987, Biochemical and biophysical research communications.

[15]  D. Gardner,et al.  Thyroid hormone increases rat atrial natriuretic peptide messenger ribonucleic acid accumulation in vivo and in vitro. , 1987, Molecular endocrinology.

[16]  D. Drucker,et al.  Glucagon gene transcription in an islet cell line is regulated via a protein kinase C-activated pathway. , 1987, The Journal of biological chemistry.

[17]  H. Gharib,et al.  Atrial natriuretic peptide in hypothyroidism. , 1987, The Journal of clinical endocrinology and metabolism.

[18]  M. Kohno,et al.  Increased circulating atrial natriuretic peptide in patients with thyrotoxicosis. , 1986, Archives of internal medicine.

[19]  J. Seidman,et al.  Biosynthesis and secretion of proatrial natriuretic factor by cultured rat cardiocytes. , 1985, Science.

[20]  C. Glembotski,et al.  Molecular forms of immunoactive atrial natriuretic peptide released from cultured rat atrial myocytes. , 1985, Biochemical and biophysical research communications.

[21]  G. Wildey,et al.  Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium. , 1985, Biochemical and biophysical research communications.

[22]  N. Minamino,et al.  Identification of rat γ atrial natriuretic polypeptide and characterization of the cDNA encoding its precursor , 1984, Nature.

[23]  B. White,et al.  Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples. , 1982, The Journal of biological chemistry.

[24]  P. Simpson,et al.  Differentiation of Rat Myocytes in Single Cell Cultures with and without Proliferating Nonmyocardial Cells: Cross‐Striations, infrastructure, and Chronotropic Response to Isoproterenol , 1982, Circulation research.

[25]  W. Rutter,et al.  Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes. , 1980, Cell.

[26]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[27]  E. Reich.  BIOCHEMISTRY OF ACTINOMYCINS. , 1963, Cancer research.