Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio).

Movement of water and cryoprotectants through the plasma membrane needs to be accelerated for successful cryopreservation of zebrafish oocytes/embryos, which are much larger than their mammalian counterparts. Aquaporin-3 is a water/solute channel that can transport not only water but also various cryoprotectants. In this study, we attempted to increase the permeability of immature zebrafish oocytes at stage III to water and cryoprotectants by exogenous expression of rat aquaporin-3. Immature zebrafish oocytes were injected with rat aquaporin-3 cRNA and cultured for 5-12 h. Permeability to water and cryoprotectants was then determined based on changes in the volumes of the oocytes in a hypertonic sucrose solution and various cryoprotectant solutions at 25 C. The permeability to water of the aquaporin-3 cRNA-injected oocytes was three times higher than that of intact and water-injected oocytes. The permeability of the aquaporin-3 cRNA-injected oocytes to ethylene glycol, glycerol, propylene glycol, and DMSO was also 2-4 times higher than that of intact oocytes. Thus, the permeability of immature zebrafish oocytes to water and cryoprotectants was enhanced by exogenous expression of aquaporin-3. Cryopreservation of teleost oocytes may be realized through a further increase in permeability.

[1]  S. Seki,et al.  The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio). , 2007, Cryobiology.

[2]  S. Seki,et al.  Expression of aquaporin-3 improves the permeability to water and cryoprotectants of immature oocytes in the medaka (Oryzias latipes). , 2006, Cryobiology.

[3]  S. Seki,et al.  Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes. , 2006, Cryobiology.

[4]  S. Seki,et al.  Japanese flounder (Paralichthys olivaceus) embryos are difficult to cryopreserve by vitrification. , 2006, Cryobiology.

[5]  S. L. Chen,et al.  Cryopreservation of flounder (Paralichthys olivaceus) embryos by vitrification. , 2005, Theriogenology.

[6]  S. Seki,et al.  Water- and cryoprotectant-permeability of mature and immature oocytes in the medaka (Oryzias latipes). , 2005, Cryobiology.

[7]  D. Rawson,et al.  Studies on cryoprotectant toxicity to zebrafish (Danio rerio) oocytes. , 2004, Cryo letters.

[8]  B. Jamieson,et al.  Fish evolution and systematics: Evidence from spermatozoa , 2004, Reviews in Fish Biology and Fisheries.

[9]  F. Kleinhans,et al.  Artificial Expression of Aquaporin-3 Improves the Survival of Mouse Oocytes after Cryopreservation1 , 2003, Biology of reproduction.

[10]  Toru Suzuki,et al.  Factors affecting the uptake of DMSO by the eggs and embryos of medaka, Oryzias latipes. , 2002, Theriogenology.

[11]  B. Pukazhenthi,et al.  Altering Fish Embryos with Aquaporin-3: An Essential Step Toward Successful Cryopreservation1 , 2002, Biology of reproduction.

[12]  Michael Brand,et al.  Keeping and raising zebrafish , 2002 .

[13]  F. Kleinhans,et al.  Overcoming a permeability barrier by microinjecting cryoprotectants into zebrafish embryos (Brachydanio rerio). , 2000, Cryobiology.

[14]  F. Kleinhans,et al.  Membrane permeability modeling: Kedem-Katchalsky vs a two-parameter formalism. , 1998, Cryobiology.

[15]  D Artemov,et al.  Characterization of a major permeability barrier in the zebrafish embryo. , 1998, Biology of reproduction.

[16]  F. Kleinhans,et al.  Chill sensitivity and cryoprotectant permeability of dechorionated zebrafish embryos, Brachydanio rerio. , 1997, Cryobiology.

[17]  E. Windhager,et al.  Cloning and expression of AQP3, a water channel from the medullary collecting duct of rat kidney. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[18]  R. Wallace,et al.  Stages of oocyte development in the zebrafish, Brachydanio rerio , 1993, Journal of morphology.

[19]  W. Rall.  Advances in the Cryopreservation of Embryos and Prospects for Application to the Conservation of Salmonid Fishes , 1993 .

[20]  R. Wallace,et al.  Oocyte maturation in the mummichog (Fundulus heteroclitus): effects of steroids on germinal vesicle breakdown of intact follicles in vitro. , 1986, General and comparative endocrinology.

[21]  M. Ashwood‐Smith,et al.  Permeability of intact and dechorionated zebra fish embryos to glycerol and dimethyl sulfoxide. , 1983, Cryobiology.

[22]  G. Perron,et al.  Volumes and Heat Capacities of Dimethylsulfoxide, Acetone, and Acetamide in Water and of some Electrolytes in these Mixed Aqueous Solvents , 1975 .