Expression Characterization, Polymorphism and Chromosomal Location of the Porcine Calsarcin-3 Gene

: Calcineurin is a calmodulin dependent protein that functions as a regulator of muscle cell growth and function. Agents capable of interacting with calcineurin could have important applications in muscle disease treatment as well as in the improvement of livestock production. Calsarcins comprise a family of muscle-specific calcineurin binding proteins which play an important role in modulating the function of calcineurin in muscle cells. Recently, we described the first two members of the calsarcin family (calsarcin-1 and calsarcin-2) in the pig. Here, we characterized the third member of the calsarcin family, calsarcin-3, which is also expressed specifically in skeletal muscle. However, unlike calsarcin-1 and calsarcin-2, the calsarcin-3 mRNA expression in skeletal muscle kept rising throughout the prenatal and postnatal development periods. In addition, radiation hybrid mapping indicated that porcine calsarcin-3 mapped to the distal end of the q arm of pig chromosome 2 (SSC2). A C/T single nucleotide polymorphism site in exon 5 was genotyped using the denaturing high performance liquid chromatography (DHPLC) method and the allele frequencies at this locus were significantly different among breeds.

[1]  Shu-lin Yang,et al.  Characterization of different expression patterns of calsarcin-1 and calsarcin-2 in porcine muscle. , 2006, Gene.

[2]  Shu-lin Yang,et al.  Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A , 2006 .

[3]  X. Li,et al.  Mapping, Tissue Distribution and Polymorphism Study of the Porcine SOCS2 and SOCS3 Genes , 2005 .

[4]  E. Olson,et al.  Mice lacking calsarcin-1 are sensitized to calcineurin signaling and show accelerated cardiomyopathy in response to pathological biomechanical stress , 2004, Nature Medicine.

[5]  M. Yerle,et al.  Sequence characterization, polymorphism and chromosomal localizations of the porcine PSME1 and PSME2 genes. , 2004, Animal genetics.

[6]  Katherine E Yutzey,et al.  Calcineurin signaling and NFAT activation in cardiovascular and skeletal muscle development. , 2004, Developmental biology.

[7]  C. Maltin,et al.  Slow fiber cluster pattern in pig longissimus thoracis muscle: implications for myogenesis. , 2003, Journal of animal science.

[8]  Shuhong Zhao,et al.  Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle Tissue between Duroc and Erhualian Pigs by mRNA Differential Display , 2003 .

[9]  E. Olson,et al.  Calsarcin-3, a Novel Skeletal Muscle-specific Member of the Calsarcin Family, Interacts with Multiple Z-disc Proteins* , 2002, The Journal of Biological Chemistry.

[10]  L. Kunkel,et al.  Myozenin: An α-actinin- and γ-filamin-binding protein of skeletal muscle Z lines , 2001 .

[11]  Simon C Watkins,et al.  Myozenin: an alpha-actinin- and gamma-filamin-binding protein of skeletal muscle Z lines. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[12]  G. Lanfranchi,et al.  FATZ, a Filamin-, Actinin-, and Telethonin-binding Protein of the Z-disc of Skeletal Muscle* , 2000, The Journal of Biological Chemistry.

[13]  E. Olson,et al.  Calsarcins, a novel family of sarcomeric calcineurin-binding proteins. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Lee Alexander,et al.  IMpRH Server: an RH mapping server available on the Web , 2000, Bioinform..

[15]  T. Meuwissen,et al.  Genetic parameters and trends of meat quality, carcass composition and performance traits in two selected lines of large white pigs , 1998 .

[16]  D. Milan,et al.  Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs , 1998, Cytogenetic and Genome Research.

[17]  W. Zhu,et al.  A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type. , 1998, Genes & development.

[18]  D. Milan,et al.  Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. , 1996, Genomics.