DNA loop anchorage region colocalizes with the replication origin located downstream to the human gene encoding lamin B2

The recently developed procedure of topoisomerase II‐mediated DNA loop excision has been used to analyze the topological organization of a human genome fragment containing the gene encoding lamin B2 and the ppv1 gene. A 3.5 kb long DNA loop anchorage/topoisomerase II cleavage region was found within the area under study. This region includes the end of the lamin B2 coding unit and an intergenic region where an origin of DNA replication was previously found. These observations further corroborate the hypothesis that DNA replication origins are located at or close to DNA loop anchorage regions. J. Cell. Biochem. 69:13–18, 1998. © 1998 Wiley‐Liss, Inc.

[1]  R. Hancock,et al.  Mapping of genomic DNA loop organization in a 500-kilobase region of the Drosophila X chromosome by the topoisomerase II-mediated DNA loop excision protocol , 1996, Molecular and cellular biology.

[2]  B. Thomsen,et al.  Different topoisomerase II antitumor drugs direct similar specific long-range fragmentation of an amplified c-MYC gene locus in living cells and in high-salt-extracted nuclei. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[3]  L. Zentilin,et al.  Fine mapping of a replication origin of human DNA. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[4]  D. Kaufman,et al.  Association of putative origins of replication with the nuclear matrix in normal human fibroblasts. , 1993, Cancer research.

[5]  K. Ariizumi,et al.  Immunoglobulin heavy chain enhancer is located near or in an initiation zone of chromosomal DNA replication. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[6]  S. Gasser,et al.  Drosophila scaffold-attached regions bind nuclear scaffolds and can function as ARS elements in both budding and fission yeasts , 1990, Molecular and cellular biology.

[7]  S. Gasser,et al.  Chromosomal ARS and CEN elements bind specifically to the yeast nuclear scaffold , 1988, Cell.

[8]  S. Razin,et al.  DNA interactions with the nuclear matrix and spatial organization of replication and transcription , 1987, BioEssays : news and reviews in molecular, cellular and developmental biology.

[9]  G. Georgiev,et al.  Replication origins are attached to the nuclear skeleton. , 1986, Nucleic acids research.

[10]  M. Buongiorno-Nardelli,et al.  The relationship between chromosomal origins of replication and the nuclear matrix during the cell cycle. , 1986, Experimental cell research.

[11]  P. Dijkwel,et al.  Permanent attachment of replication origins to the nuclear matrix in BHK-cells. , 1986, Nucleic acids research.

[12]  R. Opstelten,et al.  Organization of DNA replication in Physarum polycephalum. Attachment of origins of replicons and replication forks to the nuclear matrix. , 1983, Nucleic acids research.

[13]  M. Buongiorno-Nardelli,et al.  A relationship between replicon size and supercoiled loop domains in the eukaryotic genome , 1982, Nature.

[14]  S. Razin,et al.  Specificity and functional significance of DNA interaction with the nuclear matrix: new approaches to clarify the old questions. , 1995, International review of cytology.

[15]  P. Dijkwel,et al.  Origins of replication and the nuclear matrix: the DHFR domain as a paradigm. , 1995, International review of cytology.

[16]  R. Hancock,et al.  Structural-functional organization of chromosomal DNA domains. , 1993, Cold Spring Harbor symposia on quantitative biology.