Biomass Production Potential of a Wastewater Alga Chlorella vulgaris ARC 1 under Elevated Levels of CO2 and Temperature

The growth response of Chlorella vulgaris was studied under varying concentrations of carbon dioxide (ranging from 0.036 to 20%) and temperature (30, 40 and 50°C). The highest chlorophyll concentration (11 μg mL–1) and biomass (210 μg mL–1), which were 60 and 20 times more than that of C. vulgaris at ambient CO2 (0.036%), were recorded at 6% CO2 level. At 16% CO2 level, the concentrations of chlorophyll and biomass values were comparable to those at ambient CO2 but further increases in the CO2 level decreased both of them. Results showed that the optimum temperature for biomass production was 30°C under elevated CO2 (6%). Although increases in temperature above 30°C resulted in concomitant decrease in growth response, their adverse effects were significantly subdued at elevated CO2. There were also differential responses of the alga, assessed in terms of NaH14CO3 uptake and carbonic anhydrase activity, to increases in temperature at elevated CO2. The results indicated that Chlorella vulgaris grew better at elevated CO2 level at 30°C, albeit with lesser efficiencies at higher temperatures.

[1]  M. Badger,et al.  The CO2concentrating mechanism in cyanobactiria and microalgae , 1992 .

[2]  J. Moroney,et al.  Effect of Carbonic Anhydrase Inhibitors on Inorganic Carbon Accumulation by Chlamydomonas reinhardtii. , 1985, Plant physiology.

[3]  G Charles Dismukes,et al.  Aquatic phototrophs: efficient alternatives to land-based crops for biofuels. , 2008, Current opinion in biotechnology.

[4]  Chiun-Hsun Chen,et al.  Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. , 2009, Bioresource technology.

[5]  J. Moroney,et al.  How Do algae concentrate CO2 to increase the efficiency of photosynthetic carbon fixation? , 1999, Plant physiology.

[6]  C. Lan,et al.  Biofuels from Microalgae , 2008, Biotechnology progress.

[7]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[8]  E. Fernández,et al.  Constitutive expression of nitrate reductase changes the regulation of nitrate and nitrite transporters in Chlamydomonas reinhardtii , 1996 .

[9]  Beatriz P. Nobre,et al.  Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae , 2003 .

[10]  J. Costa,et al.  Biofixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. , 2007, Journal of biotechnology.

[11]  M. Badger,et al.  The CO2 concentrating mechanism in cyanobacteria and microalgae , 1992 .

[12]  F. Tabita,et al.  Isolation and characterization of heterocysts from Anabaena sp. strain CA , 1982, Archives of Microbiology.

[13]  Rafael Borja,et al.  BIOALGA reactor: preliminary studies for heavy metals removal , 2002 .

[14]  Hugo Scheer,et al.  Chlorophylls and Carotenoids , 2004 .

[15]  G. Dixon,et al.  Role of intracellular carbonic anhydrase in inorganic-carbon assimilation by Porphyridium purpureum , 1987, Planta.

[16]  Isao Karube,et al.  Tolerance of microalgae to high CO2 and high temperature , 1992 .

[17]  D. F. Cox,et al.  Statistical Procedures for Agricultural Research. , 1984 .

[18]  J. Costa,et al.  Isolation and selection of microalgae from coal fired thermoelectric power plant for biofixation of carbon dioxide , 2007 .

[19]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[20]  G. Mackinney,et al.  ABSORPTION OF LIGHT BY CHLOROPHYLL SOLUTIONS , 1941 .

[21]  H. Rogers,et al.  A field technique for the study of plant responses to elevated carbon dioxide concentrations , 1983 .

[22]  M. Kodama,et al.  A new species of highly CO2-tolerant fast-growing marine microalga suitable for high-density culture , 1993 .

[23]  E. M. Kondilia,et al.  Biofuel implementation in East Europe : Current status and future prospects , 2007 .

[24]  K. Gao,et al.  Impacts of elevated CO2 concentration on biochemical composition, carbonic anhydrase, and nitrate reductase activity of freshwater green algae , 2005 .

[25]  I. Karube,et al.  Chlorella strains from hot springs tolerant to high temperature and high CO2 , 1995 .

[26]  S. Miyachi,et al.  Historical perspective on microalgal and cyanobacterial acclimation to low- and extremely high-CO2 conditions , 2004, Photosynthesis Research.

[27]  R. Gutiérrez,et al.  Trend analysis using nonhomogeneous stochastic diffusion processes. Emission of CO2; Kyoto protocol in Spain , 2006 .

[28]  Aikaterini Papazi,et al.  Bioenergetic changes in the microalgal photosynthetic apparatus by extremely high CO2 concentrations induce an intense biomass production. , 2008, Physiologia plantarum.

[29]  E. DeLucia,et al.  Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide , 2004, Photosynthesis Research.

[30]  Yuriy Román‐Leshkov,et al.  Production of dimethylfuran for liquid fuels from biomass-derived carbohydrates , 2007, Nature.

[31]  C. Lan,et al.  CO2 bio-mitigation using microalgae , 2008, Applied Microbiology and Biotechnology.

[32]  Peter Lindblad,et al.  BioCO2 - a multidisciplinary, biological approach using solar energy to capture CO2 while producing H2 and high value products. , 2007, Biomolecular engineering.

[33]  H. Chu,et al.  A batch study on the bio-fixation of carbon dioxide in the absorbed solution from a chemical wet scrubber by hot spring and marine algae. , 2007, Chemosphere.