Optical clearing in photoacoustic flow cytometry.

Clinical applications of photoacoustic (PA) flow cytometry (PAFC) for detection of circulating tumor cells in deep blood vessels are hindered by laser beam scattering, that result in loss of PAFC sensitivity and resolution. We demonstrate biocompatible and rapid optical clearing (OC) of skin to minimize light scattering and thus, increase optical resolution and sensitivity of PAFC. OC effect was achieved in 20 min by sequent skin cleaning, microdermabrasion, and glycerol application enhanced by massage and sonophoresis. Using 0.8 mm mouse skin layer over a blood vessel in vitro phantom we demonstrated 1.6-fold decrease in laser spot blurring accompanied by 1.6-fold increase in PA signal amplitude from blood background. As a result, peak rate for B16F10 melanoma cells in blood flow increased 1.7-fold. By using OC we also demonstrated the feasibility of PA contrast improvement for human hand veins.

[1]  Valery V Tuchin,et al.  Photoacoustic flow cytometry: principle and application for real-time detection of circulating single nanoparticles, pathogens, and contrast dyes in vivo. , 2007, Journal of biomedical optics.

[2]  M. Kinnunen,et al.  Effect of glucose on photoacoustic signals at the wavelengths of 1064 and 532 nm in pig blood and intralipid , 2005 .

[3]  Vladimir P. Zharov,et al.  Combination of photodynamic and ultrasonic therapy for treatment of infected wounds in animal model , 2006, SPIE BiOS.

[4]  Y. Machida,et al.  Influence of low-frequency massage device on transdermal absorption of ionic materials. , 2005, International journal of pharmaceutics.

[5]  H. Cottet,et al.  Size and charge characterization of polymeric drug delivery systems by Taylor dispersion analysis and capillary electrophoresis , 2013, Analytical and Bioanalytical Chemistry.

[6]  E. Hale,et al.  Laser skin resurfacing, chemical peels, and other cutaneous treatments of the brow and upper lid. , 2013, Clinics in plastic surgery.

[7]  Sung Bin Cho,et al.  Combination of 595-nm pulsed dye laser, long-pulsed 755-nm alexandrite laser, and microdermabrasion treatment for keratosis pilaris: retrospective analysis of 26 Korean patients , 2013, Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology.

[8]  Dan Zhu,et al.  Enhanced optical clearing of skin in vivo and optical coherence tomography in-depth imaging. , 2012, Journal of biomedical optics.

[9]  Kiwoon Kwon,et al.  Evaluation of laser beam profile in soft tissue due to compression, glycerol, and micro‐needling , 2008, Lasers in Surgery and Medicine.

[10]  Chung-Hong Hu,et al.  Microdermabrasion as a Novel Tool to Enhance Drug Delivery via the Skin: An Animal Study , 2006, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[11]  Mazen A. Juratli,et al.  Nonlinear photoacoustic signal amplification from single targets in absorption background , 2013, Photoacoustics.

[12]  Dan Zhu,et al.  In vivo skin optical clearing by glycerol solutions: mechanism , 2009, Journal of biophotonics.

[13]  M. Kar,et al.  Giant linear syringocystadenoma papilliferum of the back. , 2012, Indian journal of dermatology, venereology and leprology.

[14]  V V Tuchin,et al.  Optical clearing of skin using flashlamp‐induced enhancement of epidermal permeability , 2006, Lasers in surgery and medicine.

[15]  A. Armstrong,et al.  Current state of acne treatment: highlighting lasers, photodynamic therapy, and chemical peels. , 2011, Dermatology online journal.

[16]  S. Shen,et al.  Lasers and microdermabrasion enhance and control topical delivery of vitamin C. , 2003, The Journal of investigative dermatology.

[17]  C. Sălăvăstru,et al.  Chemical peeling with trichloroacetic acid and lactic acid for infraorbital dark circles , 2013, Journal of cosmetic dermatology.

[18]  Junjie Yao,et al.  Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth. , 2013, Optics letters.

[19]  K. Knutson,et al.  Percutaneous Absorption Enhancement of an Ionic Molecule by Ethanol–Water Systems in Human Skin , 1990, Pharmaceutical Research.

[20]  Qingming Luo,et al.  Recent progress in tissue optical clearing , 2013, Laser & photonics reviews.

[21]  Valery V Tuchin,et al.  In vivo fiber‐based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles , 2009, Journal of biophotonics.

[22]  K. Ferrara,et al.  Ultrasonic Enhancement of Drug Penetration in Solid Tumors , 2013, Front. Oncol..

[23]  Ruikang K. Wang,et al.  Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging. , 2008, Journal of biomedical optics.

[24]  Bernard Choi,et al.  Revisiting optical clearing with dimethyl sulfoxide (DMSO) , 2009, Lasers in surgery and medicine.

[25]  V. Tuchin,et al.  A Clear Vision for Laser Diagnostics (Review) , 2007, IEEE Journal of Selected Topics in Quantum Electronics.

[26]  Qingming Luo,et al.  Enhancement of skin optical clearing efficacy using photo‐irradiation , 2010, Lasers in surgery and medicine.

[27]  M. Muir.  DMSO: Many Uses, Much Controversy , 1996 .

[28]  Valery V Tuchin,et al.  In vivo photoacoustic flow cytometry for monitoring of circulating single cancer cells and contrast agents. , 2006, Optics letters.

[29]  Mustafa Sarimollaoglu,et al.  In vivo ultra‐fast photoacoustic flow cytometry of circulating human melanoma cells using near‐infrared high‐pulse rate lasers , 2011, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[30]  Valery V Tuchin,et al.  Photoinduced cell morphology alterations quantified within adipose tissues by spectral optical coherence tomography , 2013, Journal of biomedical optics.

[31]  Jongbum Seo,et al.  Enhancement of transdermal delivery of glycerol by micro-needling method combined with sonophoresis , 2009, BiOS.

[32]  Vladimir P Zharov,et al.  Photoacoustic flow cytometry. , 2012, Methods.

[33]  Valery V Tuchin,et al.  Tissue optical immersion clearing , 2010, Expert review of medical devices.

[34]  Eric F Bernstein,et al.  Laser treatment of tattoos. , 2006, Clinics in dermatology.

[35]  Bernard Choi,et al.  Optical clearing of in vivo human skin: Implications for light‐based diagnostic imaging and therapeutics , 2004, Lasers in surgery and medicine.

[36]  Christopher G. Rylander,et al.  Mechanical tissue optical clearing devices: Enhancement of light penetration in ex vivo porcine skin and adipose tissue , 2008, Lasers in surgery and medicine.

[37]  Valery V Tuchin,et al.  Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. , 2007, World journal of gastroenterology.

[38]  H. G. Rylander,et al.  Use of an agent to reduce scattering in skin , 1999, Lasers in surgery and medicine.

[39]  Hyunjin Park,et al.  Sonophoresis in transdermal drug deliverys. , 2014, Ultrasonics.

[40]  Jongbum Seo,et al.  A physical method to enhance transdermal delivery of a tissue optical clearing agent: Combination of microneedling and sonophoresis , 2010, Lasers in surgery and medicine.

[41]  K. Ariga,et al.  Emerging pressure-release materials for drug delivery , 2013, Expert opinion on drug delivery.

[42]  Qingming Luo,et al.  Optical clearing agents improve photoacoustic imaging in the optical diffusive regime. , 2013, Optics letters.

[43]  G. Altshuler,et al.  Characterization of an optimized light source and comparison to pulsed dye laser for superficial and deep vessel clearance , 2011, Lasers in surgery and medicine.

[44]  Huajiang Wei,et al.  Synergistic Effect of Ultrasound and Thiazone–PEG 400 on Human Skin Optical Clearing In Vivo , 2010, Photochemistry and photobiology.

[45]  Vladimir P Zharov,et al.  In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser. , 2009, Cancer research.

[46]  Myllyl¨a Risto,et al.  Pulsed Photoacoustic Techniques and Glucose Determination in Human Blood and Tissue , 2002 .

[47]  Valery V. Tuchin,et al.  Transcutaneous delivery of micro- and nanoparticles with laser microporation , 2013, Journal of biomedical optics.

[48]  Y-P Fang,et al.  Enhancement of topical 5‐aminolaevulinic acid delivery by erbium:YAG laser and microdermabrasion: a comparison with iontophoresis and electroporation , 2004, The British journal of dermatology.

[49]  D. Goldberg,et al.  Evaluation of histologic and electron microscopic changes after novel treatment using combined microdermabrasion and ultrasound‐induced phonophoresis of human skin , 2008, Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology.

[50]  A. Kauvar.  Successful treatment of melasma using a combination of microdermabrasion and Q‐switched Nd:YAG lasers , 2012, Lasers in surgery and medicine.

[51]  P. Friedman,et al.  595-nm long pulsed dye laser and 1450-nm diode laser in combination with intralesional triamcinolone/5-fluorouracil for hypertrophic scarring following a phenol peel. , 2010, Journal of the American Academy of Dermatology.

[52]  E Duco Jansen,et al.  Effect of optical tissue clearing on spatial resolution and sensitivity of bioluminescence imaging. , 2006, Journal of biomedical optics.

[53]  H. Kar,et al.  A comparative study on efficacy of high and low fluence Q-switched Nd:YAG laser and glycolic acid peel in melasma. , 2012, Indian journal of dermatology, venereology and leprology.

[54]  Bernard Choi,et al.  Enhancement of optical skin clearing efficacy using a microneedle roller. , 2008, Journal of biomedical optics.

[55]  Valery V. Tuchin,et al.  Effect of ethanol on the transport of methylene blue through stratum corneum , 2008 .

[56]  Christopher G. Rylander,et al.  Mechanical tissue optical clearing technique increases imaging resolution and contrast through Ex vivo porcine skin , 2011, Lasers in surgery and medicine.

[57]  Valery V. Tuchin,et al.  The characteristic time of glucose diffusion measured for muscle tissue at optical clearing , 2013 .

[58]  Valery V Tuchin,et al.  Optical clearing of human skin: comparative study of permeability and dehydration of intact and photothermally perforated skin. , 2008, Journal of biomedical optics.

[59]  Mikhail Yu Kirillin,et al.  In vivo study of the effect of mechanical compression on formation of OCT images of human skin , 2010, Journal of biophotonics.