Influence of IL-20 on lumbar disc degeneration:An experimental study

Objective: To determine the influence of IL-20 on the development of lumbar degeneration. Methods: The study design was prospective and carried out in Tianjin Fourth center Hospital, Tianjin, China between Jan 2012 and Jan 2014. Sixty-nine patients with degenerative disc disease treated surgically were included in experimental group, and fifteen patients with normal discs were included in control group. The evaluation of disc degeneration was performed using T2-weighted sagittal MRI according to the Modified Pfirrmann Grading System. After surgery, the intervertebral disc in both groups was collected and the content of proteoglycan and IL-20 were measured, the correlation between the content of IL-20, proteoglycan and the degeneration grade of lumbar disc was analyzed. Results: Compared to control group, the content of proteoglycan in experimental group is significantly lower (P=0.000), but IL-20 is significantly higher (P=0.001). In addition, with the advance of intervertebral disc degeneration, the content of IL-20 increase, while proteoglycan decrease gradually. There is significant correlation between the content of proteoglycan (p=0.001), IL-20 (p=0.002) and the degeneration grade of lumbar disc. Conclusion: In patients with degenerative disc disease, the content of IL-20 and proteoglycan has significant correlation with degeneration grade of lumbar disc, and IL-20 may promote the degeneration of lumbar disc by affecting the synthesis of proteoglycan.

[1]  Shujie Tang,et al.  Does anterior lumbar interbody fusion promote adjacent degeneration in degenerative disc disease? A finite element study , 2011, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[2]  A. Freemont,et al.  The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain. , 2008, Rheumatology.

[3]  R. Lin,et al.  IL-20 May Contribute to the Pathogenesis of Human Intervertebral Disc Herniation , 2008, Spine.

[4]  Ping Chung Leung,et al.  Modified Pfirrmann Grading System for Lumbar Intervertebral Disc Degeneration , 2007, Spine.

[5]  L. Dai,et al.  Biologic Response of the Intervertebral Disc to Static and Dynamic Compression In Vitro , 2007, Spine.

[6]  L. Dai,et al.  The cell biology of intervertebral disc aging and degeneration , 2007, Ageing Research Reviews.

[7]  A. Hansen,et al.  IL-20 is an arteriogenic cytokine that remodels collateral networks and improves functions of ischemic hind limbs , 2007, Proceedings of the National Academy of Sciences.

[8]  I. Stokes,et al.  Measurements of Proteoglycan and Water Content Distribution in Human Lumbar Intervertebral Discs , 2007, Spine.

[9]  Yu-Hsiang Hsu,et al.  IL-20: biological functions and clinical implications. , 2006, Journal of biomedical science.

[10]  A. Borthakur,et al.  Assessment of Human Disc Degeneration and Proteoglycan Content Using T1&rgr;-weighted Magnetic Resonance Imaging , 2006, Spine.

[11]  M. Aebi,et al.  The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration. , 1996, The Journal of clinical investigation.

[12]  R. Santos-Jesus,et al.  Serum cytokine levels in patients with chronic low back pain due to herniated disc: analytical cross-sectional study , 2010, Sao Paulo medical journal = Revista paulista de medicina.