Effects of Different Carbon Sources on Pectinase Pr oduction by Penicillium oxalicum

Currently, obtaining enzymatic preparations with lo w production costs is the ultimate challenge for researchers worldwide. This study com pares the productivity of pectinolytic enzymes using Penicillium oxalicum strain in submerged and solid state fermentation. Agro-industrial residues used as carbon sources were beet pulp, wheat bran a nd rapeseed cake. Enzyme production was higher in both fermentation types using wheat bran. Compar ing the two types of fermentation, it was observed that enzyme activity is higher in solid st ate fermentation. The maximal activity was reached after seven days by solid state fermentation. Pecti nase activity decreased progressively after 96 days , in solid state fermentation when was used beet pulp and rapeseed cake. Optimum pH and temperature for the crude enzyme activity was obtained by wheat br n in solid state fermentation at 5 and 60°C, respectively. The crude enzyme lost 50% of its acti vity after 40 minutes, when was heated at 60°C.

[1]  Antonio Zuorro,et al.  Enzyme-assisted extraction of lycopene from tomato processing waste. , 2011, Enzyme and microbial technology.

[2]  Carmen Soto,et al.  Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes , 2010 .

[3]  B. Pradeep,et al.  Production and Characterization of Pectinase Enzyme from Penicillium chrysogenum , 2010 .

[4]  R. Lavecchia,et al.  Mild Enzymatic Method for the Extraction of Lycopene from Tomato Paste , 2010 .

[5]  Zuming Li,et al.  Separation,Purification and Characterization of Three Endo-polygalacturonases from a Newly Isolated Penicillum oxalicum , 2009 .

[6]  Eleni Gomes,et al.  Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes , 2009 .

[7]  A. Meyer,et al.  Selective release of phenols from apple skin : Mass transfer kinetics during solvent and enzyme-assisted extraction , 2008 .

[8]  G. Guebitz,et al.  Purification and mechanistic characterisation of two polygalacturonases from Sclerotium rolfsii , 2007 .

[9]  Laxmi Ananthanarayan,et al.  Enzyme aided extraction of lycopene from tomato tissues , 2007 .

[10]  Anne S. Meyer,et al.  Upgrading of grape skins: Significance of plant cell-wall structural components and extraction techniques for phenol release , 2006 .

[11]  Ling Li,et al.  Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment. , 2006, Ecotoxicology and environmental safety.

[12]  Reena Gupta,et al.  Microbial pectinolytic enzymes: A review , 2005 .

[13]  E. Gomes,et al.  Production of pectinase by solid-state fermentation with Penicillium viridicatum RFC3 , 2005 .

[14]  Peter Luginbühl,et al.  Discovery of Pectin-degrading Enzymes and Directed Evolution of a Novel Pectate Lyase for Processing Cotton Fabric* , 2005, Journal of Biological Chemistry.

[15]  C. Voget,et al.  Purification and partial characterization of an acidic polygalacturonase from Aspergillus kawachii. , 2004, Journal of biotechnology.

[16]  Ernesto Favela-Torres,et al.  Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentations , 1993, Applied Microbiology and Biotechnology.

[17]  J. Beeumen,et al.  Purification, characterisation and mode of action of an endo-polygalacturonase from the psychrophilic fungus Mucor flavus , 2003 .

[18]  E. Gomes,et al.  Solid state production of thermostable pectinases from thermophilic Thermoascus aurantiacus , 2002 .

[19]  F. Passos,et al.  Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer , 2001 .

[20]  María J. Llama,et al.  Industrial applications of pectic enzymes: a review , 1998 .

[21]  G. L. Miller Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .