Functional vascular imaging by Photoacoustic Microscopy (PAM) and its biomedical application

The hemodynamics and oxygen saturation status of vascular are very important biomarkers for disease, such as brain glioma tumor and ischemia-reperfusion ulcer. Therefore, a high spatial resolution imaging tool for vascular imaging is demanded. Conventional optical imaging modalities, including confocal microscopy and two-photon microscopy, require external contrast agent to image blood vessels and are not sensitive to oxygen saturation. The development of photoacoustic microscopy provides a contrast-free, high-spatial resolution and functional vascular imaging tool. It’s gaining more and more popularity in biomedical research. In this paper, we introduce a dual-wavelength opticalresolution photoacoustic microscopy (OR-PAM) system for functional imaging of vasculature. This system has demonstrated its application in brain glioma tumor imaging, as well as skin ischemia-reperfusion imaging.

[1]  Kijoon Lee,et al.  Optical methods for blood perfusion measurement--theoretical comparison among four different modalities. , 2015, Journal of the Optical Society of America. A, Optics, image science, and vision.

[2]  Kijoon Lee,et al.  Diffuse correlation spectroscopy with a fast Fourier transform-based software autocorrelator , 2012, Journal of biomedical optics.

[3]  Lihong V. Wang.  Multiscale photoacoustic microscopy and computed tomography. , 2009, Nature photonics.

[4]  Yang Pu,et al.  Characterization and three-dimensional localization of cancerous prostate tissue using backscattering scanning polarization imaging and independent component analysis. , 2012, Journal of biomedical optics.

[5]  Michiya Matsusaki,et al.  Seeing Elastin: A Near-Infrared Zwitterionic Fluorescent Probe for In Vivo Elastin Imaging , 2018 .

[6]  V. Ntziachristos,et al.  Molecular imaging by means of multispectral optoacoustic tomography (MSOT). , 2010, Chemical reviews.

[7]  Lihong V. Wang,et al.  Single-breath-hold photoacoustic computed tomography of the breast , 2018, Nature Communications.

[8]  E. Newell,et al.  The impact of ischemia‐reperfusion injuries on skin resident murine dendritic cells , 2018, European journal of immunology.

[9]  H. Weiss,et al.  Cerebral ischemia and reperfusion increases the heterogeneity of local oxygen supply/consumption balance , 2013, Stroke.

[10]  Jin Young Kim,et al.  Optical resolution photoacoustic microscopy based on multimode fibers. , 2018, Biomedical optics express.

[11]  Yang Pu,et al.  Synthesis of dye conjugates to visualize the cancer cells using fluorescence microscopy. , 2014, Applied optics.

[12]  Kijoon Lee,et al.  Deep tissue flowmetry based on diffuse speckle contrast analysis. , 2013, Optics letters.

[13]  F. Ginhoux,et al.  Real-time imaging of dendritic cell responses to sterile tissue injury. , 2015, The Journal of investigative dermatology.

[14]  J. Rhie,et al.  Multispectral ex vivo photoacoustic imaging of cutaneous melanoma for better selection of the excision margin , 2018, The British journal of dermatology.

[15]  Lihong V. Wang,et al.  Second generation optical-resolution photoacoustic microscopy , 2011, BiOS.

[16]  R. Alfano,et al.  Two-photon excitation microscopy using the second singlet state of fluorescent agents within the “tissue optical window” , 2013 .

[17]  R R Alfano,et al.  Time-resolved fluorescence polarization dynamics and optical imaging of Cytate: a prostate cancer receptor-targeted contrast agent. , 2008, Applied optics.

[18]  R R Alfano,et al.  Spectral Polarization Imaging of Human Prostate Cancer Tissue Using a Near-infrared Receptor-targeted Contrast Agent , 2005, Technology in cancer research & treatment.

[19]  Fuyuan Liao,et al.  Skin blood flow dynamics and its role in pressure ulcers. , 2013, Journal of tissue viability.

[20]  Yang Pu,et al.  Determination of Optical Coefficients and Fractal Dimensional Parameters of Cancerous and Normal Prostate Tissues , 2012, Applied spectroscopy.

[21]  Chris Jun Hui Ho,et al.  Noninvasive real-time characterization of non-melanoma skin cancers with handheld optoacoustic probes , 2017, Photoacoustics.

[22]  Kijoon Lee,et al.  Multi-channel deep tissue flowmetry based on temporal diffuse speckle contrast analysis. , 2013, Optics express.

[23]  Chulhong Kim,et al.  Programmable Real-time Clinical Photoacoustic and Ultrasound Imaging System , 2016, Scientific Reports.

[24]  Robert R. Alfano,et al.  Deep optical imaging of tissue using the second and third near-infrared spectral windows , 2014, Journal of biomedical optics.

[25]  K. Soo,et al.  Hemodynamic monitoring of Chlorin e6-mediated photodynamic therapy using diffuse optical measurements. , 2014, Journal of photochemistry and photobiology. B, Biology.