Biomass Estimation in Plant Cell Cultures Using an Extended Kalman Filter

Use of carbohydrate concentration measurements linked to a digital filter was studied for biomass estimation in plant cell cultures. Due to the high complexity of the biological system, the strategy followed has been to work with a simple model and to rely on the filter to adapt the values of the model parameters to changes in the system behavior. Results obtained with this approach have shown the method to be efficient for biomass estimation, both in Erlenmeyer flasks and in bioreactors with changing operational conditions.