DNA typing for BoLA class I using sequence-specific primers (PCR-SSP).

The analysis of cattle MHC (BoLA) class I gene expression is an essential component of studies on immune responses and susceptibility to disease. International BoLA workshops have generated data and reagents that allow discrimination of class I molecules at the haplotype level, but progress has been limited by difficulties encountered in defining single alleles. Our aim in this study was to develop a DNA-based system for improved identification of expressed class I alleles, utilizing available cDNA sequences derived from cattle carrying a series of serologically defined class I specificities. This method has allowed more accurate typing of animals for expression of the class I genes present within a small number of haplotypes. The method has also reliably differentiated between allelic variants (identified by prior sequence analysis) and has split existing serological specificities. The data show that MHC class I genes in cattle are more polymorphic than demonstrated by serology and biochemical analysis.

[1]  J. Williams,et al.  1D-IEF analysis of BoLA class I expression using allo-antisera reveals additional complexity. , 2009, Animal genetics.

[2]  I. Joosten,et al.  Biochemical evidence of the expression of two major histocompatibility complex class I genes on bovine peripheral blood mononuclear cells. , 2009, Animal genetics.

[3]  H. Lewin,et al.  Joint Report of the Fourth International Bovine Lymphocyte Antigen (BoLA) Workshop, East Lansing, Michigan, USA, 25 August 1990. , 2009, Animal genetics.

[4]  D. Antczak,et al.  Joint report of the Third International Bovine Lymphocyte Antigen (BoLA) Workshop, Helsinki, Finland, 27 July 1986. , 2009, Animal genetics.

[5]  R. Stone,et al.  Breed differences in the distribution of BoLA-A locus antigens in American cattle. , 2009, Animal genetics.

[6]  F. Nicholas,et al.  Two closely linked loci and one apparently independent locus code for bovine lymphocyte antigens. , 2008, Tissue antigens.

[7]  L. Andersson,et al.  Nomenclature for factors of the BoLA system, 1996: report of the ISAG BoLA Nomenclature Committee , 1997 .

[8]  P. Krausa,et al.  Genetic diversity of HLA-A2: evolutionary and functional significance. , 1996, Immunology today.

[9]  H. Rammensee,et al.  HLA-A2 subtypes are functionally distinct in peptide binding and presentation , 1995, The Journal of experimental medicine.

[10]  J. A. Stewart,et al.  POLYMORPHISM OF BOVINE MHC CLASS I GENES. JOINT REPORT OF THE FIFTH INTERNATIONAL BOVINE LYMPHOCYTE ANTIGEN (BoLA) WORKSHOP, INTERLAKEN, SWITZERLAND, 1 AUGUST 1992 , 1994, European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics.

[11]  J. Bidwell.  Advances in DNA-based HLA-typing methods. , 1994, Immunology today.

[12]  M. Stear,et al.  GENETIC ANALYSIS OF THE ANTIGENS DEFINED AT THE THIRD INTERNATIONAL BoLA WORKSHOP , 1990, Journal of immunogenetics.

[13]  John R. Young,et al.  Sequence diversity present within the 5′ upstream regions of BoLA class I genes , 1997, Immunogenetics.